Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
|---|
| PPepDB ID | PPepDB_4410 |
| Peptide Name | KGLT_PETHY |
| PMID(s) | --NA-- |
| Plant Source (Scientific Name) | Petunia hybrida |
| Plant Source (Common Name) | Petunia |
| Plant Family | Solanaceae |
| Peptide Family | --NA-- |
| Peptide Function | Signaling-peptide |
| Peptide Function Description | Direct protein sequencing; Glycosyltransferase; Signal; Transferase | Kaempferol 3-O-beta-D-galactosyltransferase precursor (EC 2.4.1.234),(F3GalTase). |
| Activity Against | --NA-- |
| IC50 value | --NA-- |
| Sequence | ALDLVLSKDKRNTELKQQIGMYKELALNAVGPSGSSAENFKKLVDIITSCEESGKKITCVMADAFMWFSGEIAEELSVGWIPLWTSAAGSLSVHVYTDLIITDEYGCIAWLDKQEPGSVAYIGFGTVATPPPNELKAMAEALEESKTPFLMGKTIGKATALPVNSFEELDPPIVEDLKSKFNNFLNVGPFNLTTPPPSANMSNYHVAVLAFPFATHAGLLLGLVQRLANALPNVTFTFFNTSKSNSSLFTNRENVEAQGIAGREDEILTFIPGFAELRLGSLPSGVVSGDLESPFSVMLHKSVLESIAAGVPVICRPFFGDHQLNAWMVEKVWKIGVKIEGGVFTKDGTMLTPHDNNIKPFNISDGVPEGYVVGKGGIEALIGLFFKSAKENIQNAMAAAVWSLKDLFKSFFPEGFLERTSEYGKIVSWAPQVQVLSHGSVGVFINHCGWN |
| Sequence Length | 451 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 48907.09 |
| Monoisotopic Molecular Weight (Da) | 48875.93 |
| Isoelectric Point (pI) | 5.18 |
| Method / Extraction | --NA-- |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | Q9SBQ8 |
| NCBI | --NA-- |
| EMBL | AF165148 |
| Link to Source Databases | SPdb132283 |
|---|
| Addtional Information | FUNCTION:Involved in the formation of flavonol glycosides required for pollen germination. Shows a strict specificity for UDP-galactose and flavonols as substrates. Acts on kaempferol, quercetin, galangin, iso-rhamnetin, kaempferide, rhamnetin, morin, myricetin and fisetin.CATALYTIC ACTIVITY:UDP-galactose kaempferol = UDP kaempferol 3-O-beta-D-galactoside.BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=1.1 uM for kaempferol; KM=0.96 uM for quercetin; KM=426 uM for UDP-galactose with kaempferol as cosubstrate; KM=387 uM for UDP-galactose with quercetin as cosubstrate; KM=8.47 uM for kaempferol 3-O-galactoside; KM=2.65 uM for quercetin 3-O-galactoside; KM=66 uM for UDP with kaempferol 3-O-beta-D-galactoside as cosubstrate; KM=102 uM for UDP with quercetin 3-O-beta-D-galactoside as cosubstrate; Vmax=75 umol/min/mg enzyme with kaempferol as substrate; Vmax=73.4 umol/min/mg enzyme with quercetin as substrate; Vmax=75.1 umol/min/mg enzyme with UDP-galactose as substrate and kaempferol as cosubstrate; Vmax=70.5 umol/min/mg enzyme with UDP-galactose as substrate and quercetin as cosubstrate; Vmax=73.5 umol/min/mg enzyme with kaempferol 3-O-beta-D- galactoside as substrate; Vmax=101 umol/min/mg enzyme with quercetin 3-O-beta-D- galactoside as substrate; Vmax=62.5 umol/min/mg enzyme with UDP as substrate and kaempferol 3-O-beta-D-galactoside as cosubstrate; Vmax=110 umol/min/mg enzyme with UDP as substrate and quercetin 3-O-beta-D-galactoside as cosubstrate; pH dependence: Optimum pH is 7.5 in the forward direction and 6.0 in the reverse direction; Temperature dependence: Optimum temperature is 50 degrees Celsius;SUBUNIT:Monomer.TISSUE SPECIFICITY:Restricted to the male gametophyte.DEVELOPMENTAL STAGE:Expressed throughout pollen development.SIMILARITY:Belongs to the UDP-glycosyltransferase family. |
