PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_4410	KGLT_PETHY	--NA--	"Petunia hybrida"	Solanaceae	--NA--	Signaling-peptide	"Direct protein sequencing; Glycosyltransferase; Signal; Transferase | Kaempferol 3-O-beta-D-galactosyltransferase precursor (EC 2.4.1.234),(F3GalTase)."	--NA--	ALDLVLSKDKRNTELKQQIGMYKELALNAVGPSGSSAENFKKLVDIITSCEESGKKITCVMADAFMWFSGEIAEELSVGWIPLWTSAAGSLSVHVYTDLIITDEYGCIAWLDKQEPGSVAYIGFGTVATPPPNELKAMAEALEESKTPFLMGKTIGKATALPVNSFEELDPPIVEDLKSKFNNFLNVGPFNLTTPPPSANMSNYHVAVLAFPFATHAGLLLGLVQRLANALPNVTFTFFNTSKSNSSLFTNRENVEAQGIAGREDEILTFIPGFAELRLGSLPSGVVSGDLESPFSVMLHKSVLESIAAGVPVICRPFFGDHQLNAWMVEKVWKIGVKIEGGVFTKDGTMLTPHDNNIKPFNISDGVPEGYVVGKGGIEALIGLFFKSAKENIQNAMAAAVWSLKDLFKSFFPEGFLERTSEYGKIVSWAPQVQVLSHGSVGVFINHCGWN	451	"Experimental evidence at protein level"	48907.09	48875.93	5.18	--NA--	"FUNCTION:Involved in the formation of flavonol glycosides required for pollen germination. Shows a strict specificity for UDP-galactose and flavonols as substrates. Acts on kaempferol, quercetin, galangin, iso-rhamnetin, kaempferide, rhamnetin, morin, myricetin and fisetin.CATALYTIC ACTIVITY:UDP-galactose kaempferol = UDP kaempferol 3-O-beta-D-galactoside.BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=1.1 uM for kaempferol; KM=0.96 uM for quercetin; KM=426 uM for UDP-galactose with kaempferol as cosubstrate; KM=387 uM for UDP-galactose with quercetin as cosubstrate; KM=8.47 uM for kaempferol 3-O-galactoside; KM=2.65 uM for quercetin 3-O-galactoside; KM=66 uM for UDP with kaempferol 3-O-beta-D-galactoside as cosubstrate; KM=102 uM for UDP with quercetin 3-O-beta-D-galactoside as cosubstrate; Vmax=75 umol/min/mg enzyme with kaempferol as substrate; Vmax=73.4 umol/min/mg enzyme with quercetin as substrate; Vmax=75.1 umol/min/mg enzyme with UDP-galactose as substrate and kaempferol as cosubstrate; Vmax=70.5 umol/min/mg enzyme with UDP-galactose as substrate and quercetin as cosubstrate; Vmax=73.5 umol/min/mg enzyme with kaempferol 3-O-beta-D- galactoside as substrate; Vmax=101 umol/min/mg enzyme with quercetin 3-O-beta-D- galactoside as substrate; Vmax=62.5 umol/min/mg enzyme with UDP as substrate and kaempferol 3-O-beta-D-galactoside as cosubstrate; Vmax=110 umol/min/mg enzyme with UDP as substrate and quercetin 3-O-beta-D-galactoside as cosubstrate; pH dependence: Optimum pH is 7.5 in the forward direction and 6.0 in the reverse direction; Temperature dependence: Optimum temperature is 50 degrees Celsius;SUBUNIT:Monomer.TISSUE SPECIFICITY:Restricted to the male gametophyte.DEVELOPMENTAL STAGE:Expressed throughout pollen development.SIMILARITY:Belongs to the UDP-glycosyltransferase family."