Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
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| PPepDB ID | PPepDB_275 |
| Peptide Name | Vigno 5 |
| PMID(s) | 22890611, 26751970, 26399495 |
| Plant Source (Scientific Name) | Viola tricolor, Viola ignobilis |
| Plant Source (Common Name) | Wild pansy, heartsease |
| Plant Family | Violaceae |
| Peptide Family | Cyclotide |
| Peptide Function | Anticancer, Antimicrobial, Cytotoxic |
| Peptide Function Description | Target site- Lipid Bilayer; 50% Cell death against Mouse fibroblasts L929 at 270 µM; Induces apoptosis in part via the mitochondrial pathway. |
| Activity Against | Human cervical carcinoma HeLa at 10 µM |
| IC50 value | --NA-- |
| Sequence | GLPLCGETCVGGTCNTPGCSCGWPVCVRN |
| Sequence Length | 29 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 2884.34 |
| Monoisotopic Molecular Weight (Da) | 2882.21 |
| Isoelectric Point (pI) | 5.96 |
| Method / Extraction | MS |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | --NA-- |
| NCBI | --NA-- |
| EMBL | --NA-- |
| Link to Source Databases | APD_02657, Cybase_786, EROP-Moscow_14300, EROP-Moscow_14301, DBAASP_8715 |
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| Addtional Information | APD analysis reveals that the sequence of this peptide most resembles (89.7% similarity) Cycloviolacin O12. Mol. Wt: 2866.3; GRAVY=0.317. Active against cancer cells: Hela. MOA: the peptide induces apoptosis by releasing cytochrome C and activating caspases in cervical cancer cells. This is one of the 13 cyclotides found previously by MALDI-TOF/TOF mass spectrometry (Hashempour et al., 2013); Induces cell death in HeLa line at micromolar concentrations (Esmaeili et al., 2016). [Control data not reported]. Apoptotic markers (chromatin condensation, DNA fragmentation, phosphatidyl serine exposure, loss of mitochondrial membrane potential). Mitochondrial (intrinsic) apoptotic pathway implicated. Fibroblast cell line was less susceptible to cell death when treated with this peptide. Can be extracted from plant material by microwave-assisted extraction, less efficient than maceration. (Farhadpour et al., 2016). Linear form identified in Viola tricolor extract. (Hellinger et al., 2015) |
