PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_275	"Vigno 5"	"22890611, 26751970, 26399495"	"Viola tricolor, Viola ignobilis"	Violaceae	Cyclotide	"Anticancer, Antimicrobial, Cytotoxic"	"Target site- Lipid Bilayer; 50% Cell death against Mouse fibroblasts L929 at 270 µM; Induces apoptosis in part via the mitochondrial pathway."	"Human cervical carcinoma HeLa at 10 µM"	GLPLCGETCVGGTCNTPGCSCGWPVCVRN	29	"Experimental evidence at protein level"	2884.34	2882.21	5.96	MS	"APD analysis reveals that the sequence of this peptide most resembles (89.7% similarity) Cycloviolacin O12. Mol. Wt: 2866.3; GRAVY=0.317. Active against cancer cells: Hela. MOA: the peptide induces apoptosis by releasing cytochrome C and activating caspases in cervical cancer cells. This is one of the 13 cyclotides found previously by MALDI-TOF/TOF mass spectrometry (Hashempour et al., 2013); Induces cell death in HeLa line at micromolar concentrations (Esmaeili et al., 2016). [Control data not reported]. Apoptotic markers (chromatin condensation, DNA fragmentation, phosphatidyl serine exposure, loss of mitochondrial membrane potential). Mitochondrial (intrinsic) apoptotic pathway implicated. Fibroblast cell line was less susceptible to cell death when treated with this peptide. Can be extracted from plant material by microwave-assisted extraction, less efficient than maceration. (Farhadpour et al., 2016). Linear form identified in Viola tricolor extract. (Hellinger et al., 2015)"