Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
|---|
| PPepDB ID | PPepDB_2207 |
| Peptide Name | Kalata B2 |
| PMID(s) | 20564013, 15654741, 28669767, 18618891, 18426225, 18557620, 17534989, 10600388, 11535828 |
| Plant Source (Scientific Name) | Oldenlandia affinis |
| Plant Source (Common Name) | Blue Diamond Flower |
| Plant Family | Rubiaceae |
| Peptide Family | Cyclic Peptide |
| Peptide Function | Antibacterial, Anticancer, Antifungal, Nematocide, Molluscicidal, Membrane-Binding, Insecticidal, Hemolytic, Antiviral, Antiparasitic |
| Peptide Function Description | The Kalata B2 showed significant activity in inhibiting development of nematode larvae and motility of adult worms; Kalata B2 show potential activity for control of gastrointestinal nematode parasites; Kalata B2 shows molluscicidal activity; Kalata B2 alter membrane permeability leading to the loss of the internal vesicle contents to the external medium; Target site- Lipid Bilayer; 50% Hemolysis against Human erythrocytes at 3.3 ±1.9 µM |
| Activity Against | Escherichia coli (MBC50: 160 µM), Escherichia coli (MBC99: >160 µM), Escherichia coli (MIC: >160 µM), Pseudomonas aeruginosa (MBC50: 40 µM), Pseudomonas aeruginosa (MBC99: 80 µM), Pseudomonas aeruginosa (MIC: 160 µM), Staphylococcus aureus (MBC50: >160 µM), Staphylococcus aureus (MBC99: >160 µM), Staphylococcus aureus (MIC: >160 µM), Candida albicans (MBC50: >160 µM), Candida albicans (MBC99: >160 µM), Candida albicans (MIC: >160 µM), Human histiocytic lymphoma U-937/GTB(IC50: 2.6 µM) |
| IC50 value | 2.6 µM |
| Sequence | GLPVCGETCFGGTCNTPGCSCTWPICTRD |
| Sequence Length | 29 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 2979.4 |
| Monoisotopic Molecular Weight (Da) | 2977.2 |
| Isoelectric Point (pI) | 4.37 |
| Method / Extraction | EST, MS, Amino acid analysis, NMR |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | P58454, B6E621 |
| NCBI | --NA-- |
| EMBL | --NA-- |
| Link to Source Databases | DBAASP_5236, PhytAMP_PHYT00198, EROP-Moscow_04060, EROP-Moscow_07306, EROP-Moscow_07305, Cybase_4, APD_01049 |
|---|
| Addtional Information | Synthesis Type: Ribosomal; Has insecticidal Activity; Toxic to Golden apple snails, serious pests of rice in South East Asia. Trp can be oxidized into N-formylkynurenine (nfk) or kynurenine (Kyn). The effect of this oxidation on peptide activity is unknown (Plan MR et al. Chembiochem 8: 1001-11). Moderately active against S. aureus (MIC 50 uM) and also reduced bacterial load in vivo (Fensterseifer IC et al., 2014). Also, MIC was reported to be 35 uM performed in 10 mM PBS supplemented with 0.1% TSB in 96-microwell plates (Printing M et al., 2010). You can rotate, zoom, and view the 3D structure here in the PDB . Updated 2/2014; Jan2015; 4/2017. |
