Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
|---|
| PPepDB ID | PPepDB_2124 |
| Peptide Name | Cyclotide chassatide C11, Uncyclotide ChaC11 |
| PMID(s) | 22467870 |
| Plant Source (Scientific Name) | Chassalia chartacea |
| Plant Source (Common Name) | --NA-- |
| Plant Family | Rubiaceae |
| Peptide Family | Cyclotide |
| Peptide Function | Antibacterial, Anticancer, Cytotoxic, Hemolytic |
| Peptide Function Description | Target site: lipid bilayer; shows 50% Hemolysis against Human erythrocytes at 13.3 µM ; Shown to possess antimicrobial, cytotoxic, and hemolytic activities and the oxidation of two Met residue causes loss of bioactivities, strengthening the importance of the hydrophobic patch for membrane interaction. |
| Activity Against | Escherichia coli (MIC: 8.5 µM), Staphylococcus aureus (MIC: >80 µM), Staphylococcus epidermidis (MIC: >80 µM), Human cervical carcinoma HeLa (IC50: 1.2 µM) |
| IC50 value | 1.2 µM |
| Sequence | IPCGESCVWIPCISGMFGCSCKDKVCYS |
| Sequence Length | 28 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 3016.6 |
| Monoisotopic Molecular Weight (Da) | 3014.26 |
| Isoelectric Point (pI) | 6.03 |
| Method / Extraction | MS, PcR |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | --NA-- |
| NCBI | --NA-- |
| EMBL | --NA-- |
| Link to Source Databases | DBAASP_4289, Cybase_875, CAMPSQ3678, APD_01992 |
|---|
| Addtional Information | Synthesis Type : Ribosomal; The compound, however, is also quite toxic (50% hemolysis of human type A blood cells: HL50 13.3 uM). Also TOXIC TO HeLa cells (50% survival: IC50 1.2 uM). This is an uncyclized cyclotide (i.e. uncyclotides). Compared to cyclotides, the C-terminal D/N critical for cyclization is lacking due to a premature stop codon. Surprisingly, this linear form is not only more abundant in the plant but also more active than the circular peptides identified in the same species. ChaC11A is an Met oxidized form of the native peptide identified during purification. This oxidized form lost its activity compared to the native form. APD update 6/21/2012; 4/2015 | Oxidation of Met led to loss of activity |
