Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
|---|
| PPepDB ID | PPepDB_2071 |
| Peptide Name | Ep-AMP1 |
| PMID(s) | 25821084 |
| Plant Source (Scientific Name) | Echinopsis pachanoi |
| Plant Source (Common Name) | San Pedro cactus |
| Plant Family | Cactaceae |
| Peptide Family | Cyclotide |
| Peptide Function | Antibacterial, Anticancer, Antifungal |
| Peptide Function Description | Target site- Lipid Bilayer, Cysteine-knot peptide shows rapid bactericidal action and liposome leakage that implicates membrane permeabilisation |
| Activity Against | Staphylococcus aureus (MBC: 1.25 µM), Escherichia coli(MBC: 10 µM), Pseudomonas aeruginosa (MBC: 80 µM), Candida albicans (MBC: >160 µM), Human histiocytic lymphoma U-937/GTB(IC50: >100 µM), Staphylococcus aureus, Staphylococcus aureus, Escherichia coli, Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Candida albicans |
| IC50 value | >100 µM |
| Sequence | CVLIGQRCDNDRGPRCCSGQGNCVPLPFLGGVCAV |
| Sequence Length | 35 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 3608.22 |
| Monoisotopic Molecular Weight (Da) | 3605.66 |
| Isoelectric Point (pI) | 7.78 |
| Method / Extraction | NMR |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | --NA-- |
| NCBI | --NA-- |
| EMBL | --NA-- |
| Link to Source Databases | DBAASP_7862, APD_02627 |
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| Addtional Information | Synthesis Type: Ribosomal; 99% Killing & 50% Killing against Staphylococcus aureus at 0.63 µM & 0.31 µM respectively; 99% Killing & 50% Killing against Escherichia coli at 2.5 µM & 2.5 µM respectively; 99% Killing & 50% Killing against Pseudomonas aeruginosa at 10 µM & 10 µM respectively; 99% Killing & 50% Killing against Candida albicans at >160 µM &160 µM respectively / APD analysis reveals that the sequence of this peptide most resembles (43.6% similarity) Mj-AMP2. Mol Wt: 3590.266; mol formula: C148H246N46O45S6; GRAVY: 0.28. Its activity was more than 500 times higher against bacterial than against eukaryotic cells. Active against S. aureus (MBC 1.25 uM), E. coli (MBC 0.31 uM), and P. aeruginosa (MBC 10 uM, 99% death; 80 uM for 100% death). Ep-AMP1 targets bacterial membranes. You can rotate, zoom, and view the 3D structure here in the PDB . Activity provided by the authors. |
