Detailed Peptide Information
This page shows detailed information of individual peptides present in PlantPepDB database. The page is majorly divided into 3 sections. The first sections contains primary information like peptide activity, source, sequence, etc. In the secondary information section user can access the tertiary structure as well as the physico-chemical properties by clicking the respective links. Further there is also link of the source database and research article from which the peptide data is retrieved. Download the information by clicking
| Primary Information |
|---|
| PPepDB ID | PPepDB_3956 |
| Peptide Name | EAFP1 |
| PMID(s) | 12067732 |
| Plant Source (Scientific Name) | Eucommia ulmoides |
| Plant Source (Common Name) | Hardy rubber tree |
| Plant Family | Eucommiaceae |
| Peptide Family | Hevein |
| Peptide Function | Antifungal |
| Peptide Function Description | --NA-- |
| Activity Against | Fungi: Phytophthora infestans, Ascochyta lycopersici (IC50=155 µg/mL), Verticillium dahliae, Gibberella zeae, Alternaria nicotianae, Fusarium moniliforme (IC50=56 µg/mL), Fusarium oxysporum (IC50=46 µg/mL) and Colletotrichum gossypii (IC50=35 µg/mL). Bacteria: no effect on the growth of the tested Gram-negative bacterium Bacillus megaterium and Gram-positive bacterium Pseudomonas syringae. |
| IC50 value | 155 µg/mL | 56 µg/mL | 46 µg/mL | 35 µg/mL |
| Sequence | QTCASRCPRPCNAGLCCSIYGYCGSGNAYCGAGNCRCQCRG |
| Sequence Length | 41 |
| Validation | Experimental evidence at protein level |
| Average Molecular Weight (Da) | 4228.82 |
| Monoisotopic Molecular Weight (Da) | 4225.66 |
| Isoelectric Point (pI) | 8.62 |
| Method / Extraction | --NA-- |
| External links (Uniprot, PDB and Source Information Database) |
|---|
| Uniprot | P83596 |
| NCBI | 32363160 |
| EMBL | --NA-- |
| Link to Source Databases | PhytAMP_PHYT00225, CAMPSQ351, APD_01164 |
|---|
| Addtional Information | The N-terminus of the peptide is blocked by pyroglutamate. This is the first finding of a plant antifungal peptide with a five-disulfide motif. The five S-S bonds are C1-C5, C2-C9, C3-C6, C4-C7, and C8-C10. EAFP1 and EAFP2 show characteristics of hevein domain and exhibit chitin-binding properties similar to the previously identified hevein-like peptides. They exhibit relatively broad spectra of antifungal activities against eight pathogenic fungi from cotton, wheat, potato, tomato and tobacco. |
