<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33736

Description Mediator of RNA polymerase II transcription subunit 13
SequenceFPQADLTGIKWKRYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPNFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILSPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISSCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSSVGSSHCSASCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSAQKWIKFSSVSDGFSSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEETADKVASWDFVEATQRTNCSCLRHKNLKPRNPGQQGQAPSLSQQQQVLPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDTDSASQRLVISAPDSQVRFTNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLIPSKPVEDGIDSLSQSFPAQFQEAVEPTIYVGTAVNLEEDEANIAWKYYKVPKKKDLEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQYLSAIASDSEQEPKIDPYAFVEGDEEFLFPDKKDRQNNEREAGKKHKVEDGPSSVTVLSHEEDAMSLFSPSIKQDAPRPAGHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKSSCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQVLVGCSMFAPLKTLPSQCLPPIKLPEECIYRQSWTVGKLELLSSGPTMPFIKEGDGSTMDQEYGPAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTECGKEAEKRFEALRATSAEHVNGGLKESEKLPDELILLLQDQCTNLFSPFGAADQDPFPKIGVANNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVRSSCLHPWSKRNDVSVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDFDFLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYECCRLGQHRPISRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNSEAFSKLKLYAQVCRYDLGPYLASQPLDSSLLSQPNLVAPTSQSLITPPQMTSTGNAGTPSATLASTVSSTMTMTSGVAMSSSVATANSTLTTTSTSSSLSSNLNSGMSSNKLPSFPPFGTMNSSATGSMSTQASTVPNSQLGGQPPSALQTAGISGESSSVPTQPHPDVSESTMDRDKVGVPTDGDSHAITYPPAIVVYIIDPFTYENKDESTNSSNVWTLGLLRCFLEMVQTLPPHIKNTVSVQIVPCQYLLQPVKHEDRQIYTQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALKSPDRPECIRLYTPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNSRARRKKGSARRFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSSTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQGTDRLLSTESHDEVTNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPAIQDRRSCLPIHFVVLNQLYNFIMNML
Length2156
PositionMiddle
OrganismOvis aries (Sheep)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Artiodactyla> Ruminantia> Pecora> Bovidae> Caprinae> Ovis.
Aromaticity0.08
Grand average of hydropathy-0.408
Instability index53.47
Isoelectric point5.40
Molecular weight237466.25
Publications
PubMed=20809919

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364134
GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl
GO - Biological Function
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription coactivator activity	GO:0003713	IEA:Ensembl
GO - Biological Process
cholesterol homeostasis	GO:0042632	IEA:Ensembl
negative regulation of DNA-binding transcription factor activity	GO:0043433	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
triglyceride homeostasis	GO:0070328	IEA:Ensembl

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP33736
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     172.55|      31|      31|     944|     974|       1
---------------------------------------------------------------------------
  526-  552 (31.98/ 9.52)	DEG.V........TK.TPSTPQSqhfY.QMPtPDPLIP
  944-  974 (59.05/23.79)	GDGSTMDQE.YGPAY.TPQTHTS...F.GMP.PSSAPP
  978- 1008 (46.93/17.40)	GAG.ILPSP.STPRFpTPRTPRT...P.RTP.RGAGGP
 1013- 1044 (34.60/10.91)	GSVKYENSDlYSPAS.TPSTCRP...LnSVE.PATVP.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     569.09|     212|     529|    1045|    1291|       2
---------------------------------------------------------------------------
  589-  746 (200.10/118.18)	....................................................................................EDEANIAWKYYKVPKKKDLEFLPPQLPSDKFKDdpvGPFgQESVTSVTELMV....QCK...KPLKVSD.ELVQQYQIKNQYLSAiasdSEQEPKIDPYAFVEGDEEFLfPDKKDRQNNER..EAGKKHKvEDGPSSVTVLSHEEDAMSLFS.PSIKQDAPRPAGHARP
 1045- 1282 (323.01/296.77)	SIP.EAHSlyvnlILSESVMNLFK...DCNFDSCCIcvcnmNIKGADVgVYIPDP.TQEAQYRCTCGfSAVMNrkFGNNSgLFLEDELDIIGRNTECGKEAEKRFEALRATSAEHVN...GGL.KESEKLPDELILllqdQCTnlfSPFGAADqDPFPKIGVANNWVRV....EERDCCNDCYLALEHGRQFM.DNMSGGKVDEAlvRSSCLHP.WSKRNDVSVQCSQDILRMLLSlQPVLQDAIQKKRTVRP
 1584- 1653 (45.98/16.73)	SVPtQPHP.....DVSESTMDRDKvgvPTDGDSHAI.....TYPPAIV.VYIIDPfTYENKDESTNS.SNVWT..LGLLR.CFLE........................................................................................................................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.78|      16|      18|    1490|    1505|       3
---------------------------------------------------------------------------
 1490- 1505 (28.62/17.90)	TVSSTMTMTS.GVAMSS
 1509- 1525 (22.16/12.03)	TANSTLTTTStSSSLSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     101.97|      29|     205|     289|     318|       5
---------------------------------------------------------------------------
  289-  318 (46.28/26.11)	VHQVPaSTRDPAMSSVTLTPPTSPEEVQTV
  497-  525 (55.69/28.41)	VAKTP.QMHGTEMANSPQPPPLSPHPCDVV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.77|      14|      68|    1471|    1489|       6
---------------------------------------------------------------------------
 1465- 1478 (23.91/ 6.62)	SQSLIT.PPQMTSTG
 1533- 1547 (21.86/19.12)	SNKLPSfPPFGTMNS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     117.25|      34|      40|     827|     862|       8
---------------------------------------------------------------------------
  827-  862 (57.57/43.50)	GF.SPMNMNNKEYGSMDTTPGGTVLEGnsSSIGAQFK
  868-  902 (59.68/38.54)	GFcSPKPSEIKDFSYVYKPENCQVLVG..CSMFAPLK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     130.31|      42|     441|    1414|    1462|      10
---------------------------------------------------------------------------
 1414- 1462 (53.14/54.83)	EWFSQAADGNSEAFSKlKLyAQVCRYdLGPYLASQPldsSLLSqPNLVA
 1857- 1898 (77.17/46.43)	DWSCLLSRRNLQSLSK.RL.KDMCRM.CGISAADSP...SILS.ACLVA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.27|      25|    1193|     166|     193|      14
---------------------------------------------------------------------------
  166-  193 (40.62/34.54)	PVYLLSEEHITLAQqsnSPF......QVILSPFG
 1315- 1345 (41.65/26.48)	PTFLLGYDFDFLVL...SPFalpyweRLMLEPYG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.14|      23|     466|     319|     356|      17
---------------------------------------------------------------------------
  319-  341 (40.76/50.70)	DPQSAQKWIKFSSVSDGFSSDST
  365-  387 (40.38/16.78)	NMNRAQNKRKYSASSGGLCEEET
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33736 with Med13 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) CSCLRHKNLKPRNPGQQGQAPSLSQQQQVLPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDTDSASQRLVISAPDSQVRFTNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLIPSKPVEDG
2) DLDNLFNSDEDELTPGSKKSANGSDDKSSCKESKT
3) FPDKKDRQNNEREAGKKHKVEDGPSSVTVLSHEE
4) GPTMPFIKEGDGSTMDQEYGPAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENS
5) GSPVHSPGSHYPHGGDAGKGQGTDRLLSTESH
6) TSGVAMSSSVATANSTLTTTSTSSSLSSNLNSGMSSNKLPSFPPFGTMNSSATGSMSTQASTVPNSQLGGQPPSALQTAGISGESSSVPTQPHPDVSESTMDRDKVGVPTDGD
7) VAPTSQSLITPPQMTSTGNAGTPSATLASTVSS
405
763
690
935
2003
1498
1461
559
797
723
1020
2034
1610
1493










Molecular Recognition Features






MoRF SequenceStartStop




1) AFVEGD
680
685