<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33728

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAVMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYASPSDLLDDKTTSPIILHENNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSLVTSANSVDLPACFFLKFPQPLPVSRAFVQKIQNCTGIPLFETQPTYVPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKTKKKKSSRLPPDKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDPDVFQANNNENPYTDPADLIADAAGSPSSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNSQADTVDFSIIAVAGKALGPTDLMEHHSGSQSPLLTTGDLGKEKTQKRVKEGNGASNSSLSGTGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSLSSASNSGKMKSSKSEGSSSSKLSSSIYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSTGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPMDGQMGVSTNSSHPVSSKHNMSGGEFQGKREKSDKDKSKVSTSGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGEGGGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSTDRPSRLSPDFMIGEEDDDLMDVALIGN
Length1576
PositionMiddle
OrganismOvis aries (Sheep)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Laurasiatheria> Artiodactyla> Ruminantia> Pecora> Bovidae> Caprinae> Ovis.
Aromaticity0.04
Grand average of hydropathy-0.671
Instability index52.95
Isoelectric point8.88
Molecular weight167879.71
Publications
PubMed=20809919

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleolus	GO:0005730	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
DNA binding	GO:0003677	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	IEA:Ensembl
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription corepressor activity	GO:0003714	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IEA:Ensembl

GO - Biological Process
androgen biosynthetic process	GO:0006702	IEA:Ensembl
angiogenesis	GO:0001525	IEA:Ensembl
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	IEA:Ensembl
cellular response to epidermal growth factor stimulus	GO:0071364	IEA:Ensembl
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IEA:Ensembl
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	IEA:Ensembl
keratinocyte differentiation	GO:0030216	IEA:Ensembl
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	IEA:Ensembl
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	IEA:Ensembl
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	IEA:Ensembl
negative regulation of apoptotic process	GO:0043066	IEA:Ensembl
negative regulation of keratinocyte proliferation	GO:0010839	IEA:Ensembl
negative regulation of neuron differentiation	GO:0045665	IEA:Ensembl
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:Ensembl
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IEA:Ensembl
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IEA:Ensembl
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP33728
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            11|     509.79|      49|      50|     979|    1027|       2
---------------------------------------------------------------------------
  623-  673 (42.18/ 9.47)	T...PPHHTPPPVS.SM.A.GNTKNHP.MLMNL..LK.............DNP......AQ......DFST....LYGSSPlerQNSSS
  724-  766 (45.31/10.81)	F...DVNMTADTLD.T..P....HITP.APSQC..S...............TP......PT.....TYPQP....VPHPQP...SIQRM
  789-  854 (39.82/ 8.46)	E...ASKLPSTSDD.CP.PiGTPVRDS.SSSGH..SQsalfdpdvfqannNEN......PY.....TDPADliadAAG.SP...SSDSP
  979- 1027 (86.17/28.33)	S...NSSLSGTGLD.SK.P.GKRSRTP.SNDGK..SK.............DKP......PK.....RKKAD....TEGKSP...SHSSS
 1030- 1083 (45.70/10.98)	P...FTPPTSTG.G.SKsP.GSSGRSQ.TPPGV..AT.............PPI......PKitiqiPKGTV....MVGKPS...SHSQY
 1177- 1228 (44.70/10.55)	SslmNPSLSKPNI...S.P..SHSRPP.GGSDKlaSP.............MKP......VP.....GTPPS....SKAKSP..iSSGSG
 1230- 1263 (40.95/ 8.94)	S...H..MSGT..S.SS.T.GMKSSSGlGSSGS..LS.............QKT......P....................P...S.SNS
 1267- 1300 (45.72/10.99)	S...SSSFSSSG........SSMSSSQ.NQHGS..SK.............GKS......PS.....R..........NKKP.......S
 1304- 1338 (36.29/ 6.95)	V...IDKLKHGVVT.SG.P.GGEDPMD.GQMGV..ST.............N............................S....SHPVS
 1360- 1404 (42.07/ 9.43)	S...KVSTSGGSVDsSK.K.TSESKNV.GSTGV..AK.............III......SK.....H...D......GGSP...SIKAK
 1407- 1450 (40.89/ 8.92)	L...QKPGEGGG......E.GLRPQMA.S..SK..NY.............GSPlisgstPK.....HER........G.SP...SHSKS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     100.07|      15|      43|     298|     312|       3
---------------------------------------------------------------------------
  298-  312 (30.80/19.52)	DLPACFFL.KFPQPLP
  326-  338 (20.95/10.56)	GIP..LFE.TQPTYVP
  341-  356 (20.49/10.14)	ELITQFELsKDPDPLP
  369-  383 (27.83/16.82)	GQQHCYFL.NKDAPLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      58.81|      11|      47|    1085|    1095|       4
---------------------------------------------------------------------------
 1085- 1095 (21.16/ 9.54)	SSGSVSSSGSK
 1132- 1142 (19.44/ 7.90)	SQGSSGSSQSK
 1162- 1171 (18.21/ 6.72)	SSGS.SSTKMK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      84.37|      21|      23|     535|     557|       6
---------------------------------------------------------------------------
  537-  555 (27.72/11.81)	...K.KNLPPASSPGYGMTTGNN
  556-  577 (32.34/12.32)	PMsG.TTTPTNTFPGGPITTLFN
  602-  621 (24.30/ 7.37)	PI..lTSLLQITGNG.GSTIGSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     136.12|      44|     340|     101|     150|       7
---------------------------------------------------------------------------
  101-  150 (67.78/56.26)	CYITSDMFYVEVQlDPAGQ..LC...DVKVAHHgenpVSCpELVQQLREK..NFDEF
  443-  493 (68.34/38.53)	CPLSESRFSVSFQ.HPVNDslVCvvmDVQDSTH....VSC.KLYKGLSDAliCTDDF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      93.96|      25|      34|     883|     907|       8
---------------------------------------------------------------------------
  866-  891 (35.58/16.07)	NPDL....LNSQSQSGFG.........eeyFDE...SSQS.....GD
  892-  927 (33.01/14.38)	NDDFK..GFASQALNTLGvpmlggdngetkFKG...NSQA......D
  928-  962 (25.38/ 9.37)	TVDFSiiAVAGKALGPTD............LMEhhsGSQSpllttGD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.34|      19|     155|    1340|    1359|       9
---------------------------------------------------------------------------
 1340- 1359 (29.65/18.78)	KHNMSGGEFQGKREKsDKDK
 1499- 1517 (30.69/15.39)	KEKKKVKDKDRDRDK.DRDK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.54|      18|     858|     691|     708|      17
---------------------------------------------------------------------------
  691-  708 (34.16/20.10)	KSSRLPPDKPKHQTEDDF
 1551- 1568 (33.38/19.44)	RPSRLSPDFMIGEEDDDL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33728 with Med1 domain of Kingdom Metazoa
Unable to open file!