<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33505

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDVQMAARFERVEKALATLIDSVSRYNPTISQGNELVAADQELGKGLEELKVHQQNYERIQRLRSVTNGLDSQIKDTLTQLAQARKELGNTPATSFPSGPNYPIKYDELLAYARRISKTTMPNANVLAAAQANAIESAAKPDSGAETPNVPTPVAGTPNGVGTPAQVNGAASTPAMSGDPASQQTNATLPEGLQAHLNPLANIDFLPWPSEDLLRQGALANIQFLQDQGINPQGYDPAAEEARKKREEEEAKEREEQERLQREENERKFREQRERARIEEEKAREESFRRASVSQAQAGATSGAGSSPPPPPPQQQKQFQFMGGDDDDDDSD
Length332
PositionMiddle
OrganismPestalotiopsis fici (strain W106-1 / CGMCC3.15140)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Xylariomycetidae> Xylariales> Sporocadaceae> Pestalotiopsis.
Aromaticity0.05
Grand average of hydropathy-0.903
Instability index57.39
Isoelectric point4.81
Molecular weight36300.43
Publications
PubMed=25623211

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP33505
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.81|      20|      23|     243|     264|       1
---------------------------------------------------------------------------
  243-  264 (26.04/26.64)	RKKREEEEaKEREEQERlQREE
  267-  286 (34.77/23.81)	RKFREQRE.RARIEEEK.AREE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.89|      15|      16|     144|     158|       2
---------------------------------------------------------------------------
  144-  158 (28.66/17.35)	GAETPNVPTPVAGTP
  160-  174 (27.23/16.12)	GVGTPAQVNGAASTP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.39|      14|      16|     202|     217|       3
---------------------------------------------------------------------------
  202-  217 (20.62/17.81)	NIDFLpwPSEDLLRQG
  221-  234 (26.77/14.05)	NIQFL..QDQGINPQG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.27|      21|      28|      71|      97|       4
---------------------------------------------------------------------------
   71-   97 (26.98/32.91)	DSQIK.DTLtqLAQARKelgnTPATSFP
  101-  122 (34.29/21.46)	NYPIKyDEL..LAYARR....ISKTTMP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33505 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) NVLAAAQANAIESAAKPDSGAETPNVPTPVAGTPNGVGTPAQVNGAASTPAMSGDPASQQTNATLPEGLQAHLNPLAN
2) QGALANIQFLQDQGINPQGYDPAAEEARKKREEEEAKEREEQERLQREENERKFREQRERARIEEEKAREESFRRASVSQAQAGATSGAGSSPPPPPPQQQKQFQFMGGDDDDDDSD
125
216
202
332










Molecular Recognition Features






MoRF SequenceStartStop




1) ESFRRASVSQAQ
2) QQQKQFQFMG
286
314
297
323