<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33468

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMGGSIREEMEVALHEHRFLVTKLLESLNTASLPPSTITATRLRTVNETFNKVLEKDRDLLAAVKKLARHQVAQKELLRIEAEIENKKQKVVQYAAQLRRSQEDIANVLRKHRVVLQNAKQKSKVVLDPRDIVAYAHRIAGTTSAPKEWQPGFPMFGFMPPAPQEHMMRAGVLSRGIVAEIVTQGPEKYTSTAARVKQDGDSEAIEKLNIPIGLGASDAQIRKQIPPGWKPGDPVDLPLDALLHYMGRSFFTVHGIILPESWKPGDPLPPDAMEMLRKKFKLPEKRAALYDDEVVDDVESLRKKRKLEEGEKADEGTADSDSSSDSSDSDEEAPNTISLSLSSSEDESDDDSD
Length352
PositionMiddle
OrganismPhytophthora parasitica (strain INRA-310)
KingdomOomycetes
LineageEukaryota> Sar> Stramenopiles> Oomycota> Peronosporales> Peronosporaceae> Phytophthora.
Aromaticity0.05
Grand average of hydropathy-0.595
Instability index54.20
Isoelectric point5.55
Molecular weight39160.90
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364141

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP33468
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      95.25|      25|      30|     210|     239|       1
---------------------------------------------------------------------------
  213-  239 (45.90/41.79)	LGASDAQIRKQI.PPGWKPGDPvdLPLD
  245-  270 (49.35/28.76)	MGRSFFTVHGIIlPESWKPGDP..LPPD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      85.97|      22|      22|     310|     331|       2
---------------------------------------------------------------------------
  291-  312 (29.66/14.97)	DEVVDDVESLRKKRKLEEGEKA
  313-  334 (35.75/19.37)	DEGTADSDSSSDSSDSDEEAPN
  335-  352 (20.56/ 8.42)	TISLSLS.SSEDESDDDSD...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.86|      27|      54|      39|      65|       4
---------------------------------------------------------------------------
   39-   65 (42.74/27.50)	ATRLRTVNETFNKVLEKDRDLLAAVKK
   94-  120 (42.12/27.01)	AAQLRRSQEDIANVLRKHRVVLQNAKQ
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33468 with Med4 domain of Kingdom Oomycetes


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ESLRKKRKLEEGEKADEGTADSDSSSDSSDSDEEAPNTISLSLSSSEDESDDDSD
298
352










Molecular Recognition Features






MoRF SequenceStartStop




1) LRKKFKL
2) LRKKRKLEE
3) SDEEAPNTISLSLSSSEDESD
275
300
328
281
308
348