<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33378

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMSTPMRIASSTSVVTQPVSGQERIGEELQSVGIYQDLERYEETIQKLSESVDSFKPDLSLITKIIECDRNLYKTLEGFHEYYKIKIELDKLEEEQKEIGRKTKFMLENLNSCFQSLNELPMLEQVEFEQETMLKQREKIHSKVVLDYAMKLAKFTRFPPTFDKSMVGPNNFIWPAEDSLRKGMLAMASLKKKELLGAALDDDNNDDDGNANIDEANKQTEGSPKPEEEDVAKERRGSYEFAANGKEQSDDKPEGDADLELDLDLDLFNPDEF
Length272
PositionMiddle
OrganismKluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) (Yeast) (Candida kefyr)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kluyveromyces.
Aromaticity0.07
Grand average of hydropathy-0.781
Instability index46.70
Isoelectric point4.53
Molecular weight31141.51
Publications
PubMed=25834639

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
RNA polymerase II core promoter sequence-specific DNA binding	GO:0000979	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro
transcription by RNA polymerase II	GO:0006366	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP33378
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.62|      10|      35|      33|      42|       1
---------------------------------------------------------------------------
   33-   42 (18.24/10.97)	IYQDLERYEE
   71-   80 (18.38/11.10)	LYKTLEGFHE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.47|      12|      25|     215|     226|       2
---------------------------------------------------------------------------
  215-  226 (21.92/11.07)	ANKQTEGSPKPE
  242-  253 (21.55/10.78)	ANGKEQSDDKPE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33378 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LGAALDDDNNDDDGNANIDEANKQTEGSPKPEEEDVAKERRGSYEFAANGKEQSDDKPEGDADLELDLDLD
195
265










Molecular Recognition Features






MoRF SequenceStartStop




1) PKPEEEDVAKERRGSYEFAANGKEQSDDKPEGDADLELDLDLDLFNPDEF
223
272