<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33376

Description Mediator of RNA polymerase II transcription subunit 16
SequenceMPLISESSNESRALERKFSSASWSKTGLIAYSENSRPDCNLSLTFLETVNGTNWKFHDPYHYLIHTHMYDDISNNISSSSSNSKQTQFFYDIDFVKWNNWSLLGGELLAISDSIGNLTILAASLNEEGNTTYDSLSMLFQDTVFKVHNKFLHLVPTNGSNENKTITKLERKETKKEYMTRILDFQWCGNSKPILTPLGAMKTPHTDPPFYKNHIQNCTPIGVFHPASVKHAFVAIRTNGVIDFWYQLSNSKDFKKISLQINQGSRQNEFEWLEKASIAHMDEDQCFIIGAFSNSTQNIDFYKLKVDWNNNGGTVNDPILHLSHIYELKPETTYKSSNLIKISNLKILSRTPIPNSKPEILITYRLINKGENISTFRSIVKRYQLSQNSLDESFSEILNITPVSQRTAQYSLKVIETMELKQVLKSISAEDLCISAAFEYESGEIDIYSRNNWKLQSDTIDKNSDIYDKPFIPSMFSVGFKYQNIPTQSNIEWSIISPSMGGTLIKYYEKPLPIFRAAVNDVVSDQSRDQVHATGLALVFVILSHRNFSGEDITITIKTHVLRLQELDPDRAINFISLLISTIFGLYGLVLDGTKGTLDKALQSKAVQNILLLQMELGPHISASKNIYSVGYIAMKLRNMNIAFNGVARNVQAMIQHTSNINMLPNGKVFQFAFSKQDLIYSLLPSAKWIVMFISYVTQQLILAVNNASYFDKSFLITLFGSRIIRHLMFKVIGELKNIANIMVKFPETAFPILNESSQFFRKVINDCTVNLDSFATFISEFGSKINEVDQSTVIVQQRYEADFLIKGIAPESLSDFDKILRTAALKFLLPHVNLAVVFFTETSFLNLRWEEKFDREVLELQKPISEGLLIYDNKSSSGLNVPDVVYDDVTSEHVNIKGRSVIKKCCRCGTYTRAGYPVDGDNTIVPTSIVTKRWTALYYRNCHCTGLLYEVKLK
Length954
PositionTail
OrganismKluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275) (Yeast) (Candida kefyr)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kluyveromyces.
Aromaticity0.11
Grand average of hydropathy-0.172
Instability index39.85
Isoelectric point7.58
Molecular weight108421.69
Publications
PubMed=25834639

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:EnsemblFungi

GO - Biological Function
RNA polymerase II activating transcription factor binding	GO:0001102	IEA:EnsemblFungi

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi
regulation of establishment of protein localization to chromosome	GO:0070202	IEA:EnsemblFungi
RNA polymerase II preinitiation complex assembly	GO:0051123	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP33376
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     246.77|      78|      89|      20|     103|       1
---------------------------------------------------------------------------
   20-  103 (130.90/111.80)	SASWSKTGLIAYSENSRPDC...NLSLTFLETVngtnWKFHDPYHYLIHTHMYDDiSNNISSSSSNSKQTQFFYDI.DFvKW..NNWSLL
  111-  194 (115.88/80.55)	SDSIGNLTILAASLNEEGNTtydSLSMLFQDTV....FKVHNKFLHLVPTNGSNE.NKTITKLERKETKKEYMTRIlDF.QWcgNSKPIL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.18|      23|      91|     743|     765|       2
---------------------------------------------------------------------------
  743-  765 (41.01/31.62)	VKFPETAFPILNESSQFFRKVIN
  837-  859 (41.17/31.79)	VFFTETSFLNLRWEEKFDREVLE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     226.28|      37|      39|     434|     470|       4
---------------------------------------------------------------------------
  250-  286 (45.51/24.80)	SKDFKKISLQINQ.GS.RQNefeW.LEKASIAHM....DE..D.QCF
  288-  330 (44.80/24.29)	IGAFSNSTQNIDF.YKlKVD...WNNNGGTVNDPilhlSHIYElKPE
  349-  372 (22.66/ 8.72)	RTPIPNSKPEILItY.........RL....INKG....ENI......
  434-  470 (63.63/37.55)	SAAFEYESGEIDI.YS.RNN...WKLQSDTIDKN....SDIYD.KPF
  476-  511 (49.68/27.73)	SVGFKYQ..NIPT.QS.NIE...WSIISPSMGGT...lIKYYE.KPL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.10|      18|      19|     662|     679|       5
---------------------------------------------------------------------------
  662-  679 (32.81/21.80)	MLPNGK.VFQF.AFSKQDLI
  682-  701 (22.29/12.51)	LLPSAKwIVMFiSYVTQQLI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.40|      14|      19|     603|     616|       6
---------------------------------------------------------------------------
  603-  616 (21.86/13.14)	SKAVQNILLLQMEL
  623-  636 (23.54/14.66)	SKNIYSVGYIAMKL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.64|      11|      59|     713|     723|       7
---------------------------------------------------------------------------
  713-  723 (21.18/10.45)	SF..LITLFGSRI
  773-  785 (16.47/ 6.73)	SFatFISEFGSKI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.66|      18|      19|     385|     402|       8
---------------------------------------------------------------------------
  375-  392 (27.77/19.67)	FRSIVKRYQLSQNSLDES
  393-  410 (28.89/20.78)	FSEILNITPVSQRTAQYS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33376 with Med16 domain of Kingdom Fungi
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