<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33254

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPGIIMDDANASGSRLGLNPSNLENGIPNSSSDNGVKRGDHGDSKDRSHERNGGPFNDSNNQSDGVKSQRESTSGPLSKHAPELLHITQGFFPYAQLVNRSVQQCWNDLVDVMNELAETQVPSQNQESQPTALVNGKTGANQSAENVHKKMRLLEFAQAKRAEFIKLLVLSQWGRQAADVSKLIDIQNFIRTQHMAYTGALQRVGDMKRDLVQAQVANPDLRTAAEVLSKGKVATLPDLGYKPPKPLNAKKTLKTLQKINRIISTRLVLNDTVPSAFRTYRVHDGRVTFFVKGEFEIDLSIAEQNHSSQFFFIDLRFLYSPSSPIPKGRFFSELDLKINEILLQSGLTGCFDFVHNLVLTNQVNILFRQALALARGGWSDALHVELLHRTLIVQYWALKSGPRNWLEIGVKTGRHKSGSNNAAISGISYLDLRWMRDGKEVDHSRIQFNKEVLSMENVLRSAIALHTSHLLQSAYSKLKEGHLYASGTLALEVDMSDEEPGNCCLTVQLTKSRYLRVSVEPISGTITLSTSPTILIRQEVDRNGDKSPVEDIVSRVSRLRCIAAMEEVESHARMMGWDTVNPRGVKLDVRRIFPHNVLRFSLFSHPLWERNWIAAATSSMDGDHWWILQLKSAPSTPSRPAVGGGLPVLQSVQNVTEKLGQRQQRLDYASFANLEHSLAGLLALYANARYLEELNCVHSFPQARKLQLEPGPRVPSLFIRFEPSKLPASVQISCPTGLRKSRFLKETVCLSFQGIDSQSKRVIIVAQGQFTAPIRTVWALASNSDNSVAFQRKGCGFAMRFLVTAGNSMIGPLFDRLQRLECVLSILEALDRKNIRTRSMSLSRLDFTYGAQQSHHASIHIKGPELSHLPQEKGTVDLQSKASPLRLLLEIRFDHSNPHRRISQSLSSVLNDNGAMSGVNHMANILTLTVPLLQALDSITCGASRDSPSKVLVTARNAKVYQIYYPHHRYRFLITVGQHRDRITWILRDARTPQEKSTPGQLESKLRERIYNSQGDGWKGLGTGAVADIQKVGNLLSELDTCFTSSANDASVKAPDAATDQTGTGQRDVPNGQGAPNTESTKPAEVKSESKNDHDVIMID
Length1100
PositionTail
OrganismByssochlamys spectabilis (strain No. 5 / NBRC 109023) (Paecilomyces variotii)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Eurotiales> Thermoascaceae> Byssochlamys.
Aromaticity0.06
Grand average of hydropathy-0.366
Instability index42.33
Isoelectric point9.29
Molecular weight122206.44
Publications
PubMed=24407650

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	ARBA:ARBA00003669
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP33254
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     765.05|     218|     370|     445|     673|       1
---------------------------------------------------------------------------
  374-  431 (40.55/15.36)	..........................................................................................................................................................AR.GGWsDALH...VELLHRTL.I...VQYWALKSGP...R.NWL..EIGV...KT..GRH..................................KS...........GSNNAAISG..I....................SYLD
  445-  673 (349.32/244.63)	RIQFNKEVLSMENVLRS.AIALHTSHL.........LQSAYSKLKEGHLYASGTL.ALEVDMSDEEPGncCLTVQL.TKSRYLRVSVEpisgtITLS.TSPTILIRQE....VDRNGDKSPVEDIVS....RVSRLRciaAMEEV......ESHARMMGW.DTVNPRGVKLDVRRI.FPHNVLRFSLFSHPLWER.NWI..AAAT...SSMDGDH............................wWILQLKSAPSTP.SRPAVGGGLPVLQS..VQN.VTEKLGQRQQRLD..YASFAN
  687-  820 (132.01/76.46)	NARYLEELNCVHSFPQArKLQLEPGPR.........VPSLFIRFEPSKLPASVQI.S.............CPT.GL.RKSRFLKETV................................................................................................................................clsfqgidsqskrviivaqgqftapirtvWALASNSDNSVAfQRKGCGFAMRFLVT..AGNsMIGPLFDRLQRL.........
  821- 1048 (243.17/147.62)	.....E...CVLSILEA....LDRKNIrtrsmslsrLDFTYGA.QQSH.HASIHIkGPELSHLPQEKG....TVDLqSKASPLRLLLE.....IRFDhSNPHRRISQSlssvLNDNGAMSGVNHMANiltlTVPLLQ...ALDSItcgasrDSPSKVL.....VTARNAK..VYQIyYPHHRYRFLITVGQHRDRiTWIlrDARTpqeKSTPG..................................QLESKLRER.IYNSQGDGWKGLGTgaVAD.I.QKVGNLLSELDtcFTSSAN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     121.25|      37|     856|     196|     232|       3
---------------------------------------------------------------------------
  196-  232 (61.79/44.86)	AYTGALQRVGDMKRDLVQAQVA.NPDLRTAAEVLSKGK
 1054- 1091 (59.46/42.86)	APDAATDQTGTGQRDVPNGQGApNTESTKPAEVKSESK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     102.36|      32|      76|     241|     277|       4
---------------------------------------------------------------------------
  241-  277 (49.15/40.36)	YKPPKPLNAKKTLKTLQ.KINRIIstrlvLNDTVPSAF
  319-  351 (53.22/32.77)	YSPSSPIPKGRFFSELDlKINEIL.....LQSGLTGCF
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33254 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ANDASVKAPDAATDQTGTGQRDVPNGQGAPNTESTKPAEVKSESKNDHDVIMID
2) LAETQVPSQNQESQPTALVNGKTGANQSAENV
3) MPGIIMDDANASGSRLGLNPSNLENGIPNSSSDNGVKRGDHGDSKDRSHERNGGPFNDSNNQSDGVKSQRESTSGPLSKHAP
1047
116
1
1100
147
82










Molecular Recognition Features






MoRF SequenceStartStop




1) KLRERIY
1005
1011