<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33001

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAAAMENGAPNGSAVHTNHDRDAKLSSPRKANGVNGDVGGADKMVNGDASTRAEPLTTETPTTSQSSRMNDLPDQIQHITTNYVPLGFLLQRLAQKTHNNLQDLIRELARKPVAGSGGASGTPPALPPGTEDGSIENIEKKKALLDFARSEHANWVKALVITEWSKKASQISKLIDLENHMFEEELVRYEQAVFTMLNLKRNLNAARVPSPDLKTALHVLSRGSAGWMPDLGFITPPPLNAQEQLKKLDDISTLLSLRLNLDDHDTIPYFFRNNYSIASGRVTFKVPGEFEVDLTLASDDFSQQLWFLDLRFLFTPAPRDLTPSLRKALEDRVNNVLGLEGLAGCYRMLHEYVLTHKINELRRQVKDLARGRWIDTVRAEQLNRAIAVQYWTSRHSGGNHYNSGAAAGPTGSNNAEVRSWFIIGVHSGRDAASADLPEEQITSRLTLRWFRDNKEVKDLGDGQISAILTTLSAENEETAEGQTHDDGSTPLSATVTLCAETVLQHIVALHVEHILTGIHTKLAAKPLFAKRLATMRLRISRTDASASFLEIQLTNAGDRIWVRIDPVTGLFSLSPQKSRVVTHSEMRINAAAAGGSLGGGSTGTGGDPVEEGFVMLDNVRNLAAQEDLGRRAKSFGWQVVRNTGPGGGHLVKPDELRQVLNLRDSYQVLWLKRPDWAAPQWHVLASLSLSGDRWWLVKLAEQPTSPPPVSGLPGPGQQQQGPAATSAARIVMQMLLPMGTSPSDLDDAFFSDLTIYATGVLAQYTDAHELSQRRKMAQATRVINPYLPSRAKLPAMSIRLSDLLSAGAAAITGSQPSTTTTTINNRSMIPSTNSRGRHGQAQRWWASDFLQLLFRGVDMDGDDETNSAAGERRRRIRVLAEARLTVLDRSKFRLLGNSSRTDQDVAFNSRTGQFCLYLRAAVGESVIDTLVRRLRSIERLVGFLDAIRRESRVVHIQTVSLSRLVFSYSVGPGSPLSLEEKQSLTTEAVAAATAAANSCGRWDVSVDLSSSAQVQLQLERGCPHLRVADFLNNVANDPTTFAAVPFFLTATLSLHRALDRIEDAWEGLVMRNAGMLGIFPRALDWYVVRFLIPPTPPPGAARPRVLVLELRLKNRRSELLWQIRRLDNNSPESTGAPVPAALANAAPASADDDLGKVVRRVFDGSGPGWKGLLTAAVASPYPEKNPEESASASASAGGTGKPARNTGSTGIEDCLLQIDEGVRQLLAGSFAAASNNAAAQQRRAQALAQAQAQAQAQAQAQAQAQAQAQQQNNGGSGGNGSSGGIPQAQTARMG
Length1294
PositionTail
OrganismSporothrix schenckii (strain ATCC 58251 / de Perez 2211183) (Rose-picker's disease fungus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Ophiostomatales> Ophiostomataceae> Sporothrix.
Aromaticity0.06
Grand average of hydropathy-0.322
Instability index46.44
Isoelectric point8.74
Molecular weight140409.67
Publications
PubMed=24855299

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP33001
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.17|      18|      22|     429|     446|       1
---------------------------------------------------------------------------
  429-  446 (30.54/15.00)	RDAAS.ADLPEEQITSRLT
  451-  469 (26.63/12.30)	RDNKEvKDLGDGQISAILT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     279.90|      95|     483|     115|     244|       3
---------------------------------------------------------------------------
  125-  244 (137.47/142.08)	ALPPgTEDGSI..ENIEKKKALLDFARSEHA.NWvkaLVITEWSK......KASQISKLIDLENHMfeeelvryeqavfTMLNLKRNLNAArvPSpdlktaLHVLSR....GSAGWMPDLG..FITPPPLNA......QEQ
  605-  720 (142.43/84.32)	GGDP.VEEGFVmlDNVRNLAAQEDLGRRAKSfGW...QVVRNTGPggghlvKPDELRQVLNLRDSY.............QVLWLKRPDWAA..PQ......WHVLASlslsGDRWWLVKLAeqPTSPPPVSGlpgpgqQQQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     188.93|      59|     469|     714|     779|       4
---------------------------------------------------------------------------
  714-  779 (89.36/60.88)	GPGQqqqgPAATSAARIVMQMLLPMGTSPSDLDDAFFSDLTIYATgvlAQYTDAHELSQRRKMAQA
 1198- 1256 (99.57/58.96)	GTGK....PARNTGSTGIEDCLLQIDEGVRQLLAGSFAAASNNAA...AQQRRAQALAQAQAQAQA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.10|      12|     468|     487|     502|       8
---------------------------------------------------------------------------
  471-  482 (19.56/14.92)	LSAENEETAEGQ
  491-  502 (19.54/ 6.24)	LSATVTLCAETV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      99.52|      29|     776|     301|     335|      11
---------------------------------------------------------------------------
  306-  335 (48.96/32.89)	WFLdLRFLFTPAPRDLTPSLRK.ALEDRVNN
 1085- 1114 (50.56/20.65)	WYV.VRFLIPPTPPPGAARPRVlVLELRLKN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.31|      10|     194|     963|     973|      12
---------------------------------------------------------------------------
  963-  973 (16.77/13.29)	RLVFSYSvGPG
 1159- 1168 (21.54/12.24)	RRVFDGS.GPG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.81|      13|      19|     873|     885|      15
---------------------------------------------------------------------------
  873-  885 (20.31/13.62)	RRRIRVLAEARLT
  889-  901 (21.50/14.94)	RSKFRLLGNSSRT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.72|      10|      28|     388|     397|      17
---------------------------------------------------------------------------
  388-  397 (21.19/12.01)	VQYW..TSRHSG
  417-  428 (15.53/ 6.83)	VRSWfiIGVHSG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33001 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LTAAVASPYPEKNPEESASASASAGGTGKPARNTGSTGIEDC
2) MAAAMENGAPNGSAVHTNHDRDAKLSSPRKANGVNGDVGGADKMVNGDASTRAEPLTTETPTTSQSSRMNDLPDQI
3) NLQDLIRELARKPVAGSGGASGTPPALPPGTEDGSI
4) SFAAASNNAAAQQRRAQALAQAQAQAQAQAQAQAQAQAQAQQQNNGGSGGNGSSGGIPQAQTARMG
1173
1
100
1229
1214
76
135
1294










Molecular Recognition Features






MoRF SequenceStartStop




1) PQAQTARMG
2) WYVVRFLI
1286
1085
1294
1092