<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP33000

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMDSDDTALGLTQEDLKAIDTVRARLYQLTNSVQRLKGDVLMGGGRGDGVDSSLLPTPESIQASSFVLQQNLASLNEVMAQHAELFRRIVVHPDPMYPGRTEEQILTSLLRKKLEPDVEQVAERARETAAARGIGRTSFAESLGAKGRRRGRRHGGMEDEDEDEEDEDDEDDDDDDDDDDDEDGDDSDNDNGPREPFGLGDVWYEARRWCMDRLGQFIREEAPDVYTKQEREMGVENVRTGLRRSLEEDDEDEDDEDEDEDDEDKLKGDDDEVIMIDDGAPPTKPQTQAAAQATQPSQPPAGPAAEPEVLLWLAARGDTDLPRYVEIESQRAAAAAAAQRRM
Length341
PositionHead
OrganismSporothrix schenckii (strain ATCC 58251 / de Perez 2211183) (Rose-picker's disease fungus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Ophiostomatales> Ophiostomataceae> Sporothrix.
Aromaticity0.04
Grand average of hydropathy-1.027
Instability index55.06
Isoelectric point4.17
Molecular weight37988.54
Publications
PubMed=24855299

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP33000
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     173.78|      55|      88|     136|     195|       1
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  136-  195 (97.54/37.55)	TSFAESLGAKGRRRG.RRHggMEdedEDEEDEDDEDDDDDDDD....DDDE.....DG...............DDSDNDNGP.REP
  226-  306 (76.24/22.13)	TKQEREMGVENVRTGlRRS..LE...EDDEDEDDEDEDEDDEDklkgDDDEvimidDGapptkpqtqaaaqatQPSQPPAGPaAEP
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP33000 with Med8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ELFRRIVVHPDPMYPGRTEEQILTSLLRKK
2) IREEAPDVYTKQEREMGVENVRTGLRRSLEEDDEDEDDEDEDEDDEDKLKGDDDEVIMIDDGAPPTKPQTQAAAQATQPSQPPAGPAAEPEVLLWLAARG
3) PDVEQVAERARETAAARGIGRTSFAESLGAKGRRRGRRHGGMEDEDEDEEDEDDEDDDDDDDDDDDEDGDDSDNDNGPREPFGLGDV
83
217
115
112
316
201










Molecular Recognition Features






MoRF SequenceStartStop




1) AAEPEVLLWLAARGD
2) DDEVIMID
3) LPRYVEIE
4) REPFGLG
303
269
320
193
317
276
327
199