<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32820

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMAKSDWSDLIKSVRNALHEKRHFIVNDSTERNQVQKCLDIIQKSIKITSLQSMMERLEAITRQLGLKYTEGPSGKELFISSDMFYVEIQFNPDNGRVKEVKISHQSEPVSCPEITDVLNNSDFQEFTKHLDGLSAIYQLNADKKQKTKAYLALHALESDLSMLAQLQSSNINEPNNLVHKSPVGILEPRKGGHPMKLTFFISPYDLLDLKTKSSVPLTVDVVVKRKLGYSASVCIESSTSHKLQTVSLLSVTKTAEGKSLPQFAALSNLNSSTLPACFVLRLPKQLPMSIETLRKIQAITGIEIVSAEIMSRSVPLVDLIANSLLPASELRDLSSYHGPYYVQLPDQCHSYYMNYTLGDLKGVEVSSIPFTHPTSVPQILFILRQQVLFNVVICSCIRRVRQPSFMDSLMFEVTALSMSVVNVSFEHPTEESLATIEFDLRDITNVKCKLYDSNSFSSLCSDEYASKIMQKCFSIPVTLRAIIIKCQEKAAILREQQQKQQTRLTQQRNPKDLFFNNLSAAEDGNDHDDLPPYLSQQLLNSNQYQDLNVSLENGAFQQHQQQQQSQPMNLPVGSSQNGPYINYQQGQQSQQMQGQVQQLHQQQINQQQQLPYQQQMNQGNHRPPNNMQQGQIQGKGQSQQFHQQQMRGMNPNQQRNLMQSQQKPNAMLLNMLSDVPAANSRKDPFMYAPNTQNCQQGLQGGVQGGNITQQCIPQMNHQPAMKPKQPRKRKGTGDIKSPGSATGRSPKRKMSEDDFMRDISTPVNDFMENFGDPQLINCPSQRPPSSDPMGANNPNNSGYDVNFSNAPRSLESMLAGPKTEPGTPNSGYNNGSQNLNLGDFTADFSNSYQYPNSSMSFPGANTGQSPGPGPGPGPGPGPGPGPGPGPGPGPGPGSNLPDLNDFSNFEYTDFANPMSLPPMKPNNKRGPKRMKSAENCNENSNSLSGLSEDGSNEQQSIMKSLSDLHNLNMPEGTSSNNANKQFTPPIAKSQRGVKSGGTNGTSSDGRRCSSADVDASGMTGKRGNFGGSIEDGVALKKERKRKRAESVDSLNKAVVVSSTAAVAGVLMPPPMITSSGEVAAGVGSAYNFMDVCANGGSNQSLPPITLNVKPVLNTVNSTNGPTGKIKRPGVQGSGLNKSKFADRMINKLKGSPDASELENNGSAATYSPKSSPKVGSSKSAKIAKGGADSSGRPIKPGQKFRSQNSSLTISKTVSNSTASPPVSSSSSLTSSAPTSSGGNSAKAQGNHAKSLIAQKRRQGSLSAVIDKLTRSTNVPGGSDGSNPGEGVPKFENCSRPDSSSNSGNGPGGARFNANTGVSSSMNKIAESKMKYSRSSDQFTMRLISRNTLKFTVTKTKPSSTMPENRSKKPAISGSSSTKGSASSTTNSLKTKSVTAGLKRQLGGSAKAGQNFKGSTLVSSSSGPINSPKPGTSSSSPKASSLFMNNRSVAGKVSSSNSNNKSKVQSTEAMFDA
Length1472
PositionMiddle
OrganismTetranychus urticae (Two-spotted spider mite)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Chelicerata> Arachnida> Acari> Acariformes> Trombidiformes> Prostigmata> Eleutherengona> Raphignathae> Tetranychoidea> Tetranychidae> Tetranychus.
Aromaticity0.05
Grand average of hydropathy-0.656
Instability index51.62
Isoelectric point9.47
Molecular weight158620.16
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP32820
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.03|      15|      15|     866|     880|       1
---------------------------------------------------------------------------
  866-  880 (33.71/13.11)	PGPGPGPGPGPGPG.P
  882-  897 (28.32/ 9.58)	PGPGPGPGPGPGSNlP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     335.74|      65|      65|     534|     598|       2
---------------------------------------------------------------------------
  488-  538 (57.93/28.76)	................EKAAILREQQQ..KQQT..RL.........TQQRN....PkdlfFNNLSAAEDGND.HDDLPPY.LSQQL
  539-  599 (105.75/60.34)	LN...SNQYQDLNVSLENGAFQQHQQQ..QQSQPMNL....PV..GSSQNG....P....YINYQQGQQSQQ.MQGQVQQ.L....
  600-  646 (47.43/21.82)	.......................HQQQinQQQQ...L....PY..QQQMNQgnhrP....PNNMQQGQ..IQ.GKGQSQQfHQQQM
  649-  703 (67.54/35.11)	MN...PNQQRNL.........MQSQQK..PNAMLLNMlsdvPA..ANSRKD....P....FMYAPNTQNCQQgLQGGVQ.......
  911-  943 (30.38/10.57)	...............................ANPMSL....PPmkPNNKRG....P.....KRMKSAENCNE.N........SNSL
  946-  977 (26.71/ 8.14)	LSedgSNEQQSIMKSLSD...........LHN..LNM....PE..GTSSNN...................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     328.77|      56|      56|    1191|    1246|       3
---------------------------------------------------------------------------
 1150- 1187 (46.23/16.22)	...............GSP....D..ASELENNGS.AATYS.....PK......SSPKVGSS.....KSA...KIAKGGA
 1191- 1239 (78.09/32.88)	...............GRPI..KP..GQKFRSQNS.SLTISKTVSNST......ASPPVSSS.SSLTSSA...PTSSGGN
 1240- 1294 (57.07/21.89)	SAKAQGN............haKSliAQK.RRQGSlSAVIDK.LTRST......NVPGGSDG.SNPGEGV...PKFENCS
 1295- 1337 (42.39/14.21)	RPDSSSN........SGNG..PG..GARF...............N........ANTGVSSSmNKIAESKmkySRSSDQ.
 1345- 1386 (35.19/10.44)	.....................RN..TLKF........TVTKTKPSSTmpenrsKKPAISGS.SSTKGSA.....SSTTN
 1387- 1426 (31.14/ 8.33)	SLKTKSVtaglkrqlGGSA..KA..GQNFK..GS.TLV.......SS......SSGPINS...................
 1428- 1465 (38.65/12.25)	.....................KP..GTSSSSPKA.S...SLFMNNRS......VAGKVSSS.NSNNKSK...VQS....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     139.15|      41|      56|     342|     385|       4
---------------------------------------------------------------------------
  342-  385 (71.57/62.04)	VQLPDQCHSYYMNYTLGDLKGVEVSsipFTHPT..SVPQILFILRQ
  400-  442 (67.58/49.13)	VRQPSFMDSLMFEVTALSMSVVNVS...FEHPTeeSLATIEFDLRD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.34|      21|      64|    1009|    1032|       5
---------------------------------------------------------------------------
 1009- 1032 (26.40/26.20)	SSADVdASGMtGKRGNF......GGSiEDG
 1074- 1100 (28.94/13.93)	SSGEV.AAGV.GSAYNFmdvcanGGS.NQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     137.76|      47|      65|     723|     786|       6
---------------------------------------------------------------------------
  723-  786 (69.46/61.06)	PKQPRKRKGTG.DIKSPGSAtgRS.......PKRkmseddfmrDISTPVN............DFMENFGDPQlincpsQRPPSS
  788-  854 (68.30/30.81)	PMGANNPNNSGyDVNFSNAP..RSlesmlagPKT.........EPGTPNSgynngsqnlnlgDFTADFSNSY......QYPNSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.94|      21|     117|     984|    1007|      11
---------------------------------------------------------------------------
  984- 1007 (34.19/23.44)	PPIAksqRGVKS.....GGTNGTSSDGRR
 1102- 1127 (32.75/15.17)	PPIT...LNVKPvlntvNSTNGPTGKIKR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32820 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LISRNTLKFTVTKTKPSSTMPENRSKKPAISGSSSTKGSASSTTNSLKTKSVTAGLKRQLGGSAKAGQNFKGSTLVSSSSGPINSPKPGTSSSSPKASSLFMNNRSVAGKVSSSNSNNKSKVQSTEAMFDA
2) PPITLNVKPVLNTVNSTNGPTGKIKRPGVQGSGLNKSKFADRMINKLKGSPDASELENNGSAATYSPKSSPKVGSSKSAKIAKGGADSSGRPIKPGQKFRSQNSSLTISKTVSNSTASPPVSSSSSLTSSAPTSSGGNSAKAQGNHAKSLIAQKRRQGSLSAVIDKLTRSTNVPGGSDGSNPGEGVPKFENCSRPDSSSNSGNGPGGARFNANTGVSSSMNKIAESKMKYSRSSDQFTM
3) VSLENGAFQQHQQQQQSQPMNLPVGSSQNGPYINYQQGQQSQQMQGQVQQLHQQQINQQQQLPYQQQMNQGNHRPPNNMQQGQIQGKGQSQQFHQQQMRGMNPNQQRNLMQSQQKPNAMLLNMLSDVPAANSRKDPFMYAPNTQNCQQGLQGGVQGGNITQQCIPQMNHQPAMKPKQPRKRKGTGDIKSPGSATGRSPKRKMSEDDFMRDISTPVNDFMENFGDPQLINCPSQRPPSSDPMGANNPNNSGYDVNFSNAPRSLESMLAGPKTEPGTPNSGYNNGSQNLNLGDFTADFSNSYQYPNSSMSFPGANTGQSPGPGPGPGPGPGPGPGPGPGPGPGPGPGSNLPDLNDFSNFEYTDFANPMSLPPMKPNNKRGPKRMKSAENCNENSNSLSGLSEDGSNEQQSIMKSLSDLHNLNMPEGTSSNNANKQFTPPIAKSQRGVKSGGTNGTSSDGRRCSSADVDASGMTGKRGNFGGSIEDGVALKKERKRKRAESVDSL
1342
1102
549
1472
1340
1050










Molecular Recognition Features






MoRF SequenceStartStop




1) TMRLI
1339
1343