<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32634

Description Meiotic mRNA stability protein kinase SSN3
SequenceMYNGKDRAQNSYQPMYQRPMQVQGQQQAQSFVGKKNTIGSVHGKAPMLMANNDVFTIGPYRARKDRMRVSVLEKYEVIGYIAAGTYGKVYKAKRQINSGTNSANGSSLNGTNAKIPQFDSTQPKSSSSMDMQANTNALRRNLLKDEGVTPGRIRTTREDVSPHYNSQKQTLIKKPLTVFYAIKKFKTEKDGVEQLHYTGISQSACREMALCRELHNKHLTTLVEIFLERKCVHMVYEYAEHDLLQIIHFHSHPEKRMIPPRMVRSIMWQLLDGVSYLHQNWVLHRDLKPANIMVTIDGCVKIGDLGLARKFHNMLQTLYTGDKVVVTIWYRAPELLLGARHYTPAVDLWSVGCIFAELIGLQPIFKGEEAKLDSKKTVPFQVNQLQRILEVLGTPDQKIWPYLEKYPEYDQITKFPKYRDNLATWYHSAGGRDKHALSLLYHLLNYDPIKRIDAFNALEHKYFTESDIPVSENVFEGLTYKYPARRIHTNDNDIMNLGSRTKNNTQASGITAGAAANALGGLGVNRRILAAAAAAAAAVSGNNASDEPSRKKNRR
Length555
PositionKinase
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces.
Aromaticity0.09
Grand average of hydropathy-0.505
Instability index34.38
Isoelectric point9.59
Molecular weight62846.21
Publications
PubMed=8164691
PubMed=7877695
PubMed=9169875
PubMed=24374639
PubMed=9845373
PubMed=9702190
PubMed=10360183
PubMed=11331604
PubMed=11555651
PubMed=11226276
PubMed=12200444
PubMed=12520306
PubMed=15175151
PubMed=14749387
PubMed=15240822
PubMed=15477388
PubMed=14988503
PubMed=16076843
PubMed=16109375
PubMed=15601835
PubMed=15687503
PubMed=16630888

Function

Annotated function Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex. 
ECO:0000269	PubMed:10360183
ECO:0000269	PubMed:11226276
ECO:0000269	PubMed:11331604
ECO:0000269	PubMed:11555651
ECO:0000269	PubMed:12200444
ECO:0000269	PubMed:12520306
ECO:0000269	PubMed:14749387
ECO:0000269	PubMed:14988503
ECO:0000269	PubMed:15240822
ECO:0000269	PubMed:15601835
ECO:0000269	PubMed:15687503
ECO:0000269	PubMed:16076843
ECO:0000269	PubMed:16109375
ECO:0000269	PubMed:16630888
ECO:0000269	PubMed:7877695
ECO:0000269	PubMed:9702190
ECO:0000269	PubMed:9845373
GO - Cellular Component
mediator complex	GO:0016592	IDA:SGD
nucleus	GO:0005634	IBA:GO_Central
GO - Biological Function
ATP binding	GO:0005524	IEA:UniProtKB-KW
cyclin-dependent protein serine/threonine kinase activity	GO:0004693	IDA:SGD
metal ion binding	GO:0046872	IEA:UniProtKB-KW
protein kinase activity	GO:0004672	HDA:SGD
protein serine kinase activity	GO:0106310	IEA:RHEA
protein threonine kinase activity	GO:0106311	IEA:RHEA
RNA polymerase II CTD heptapeptide repeat kinase activity	GO:0008353	IDA:SGD
GO - Biological Process
meiotic cell cycle	GO:0051321	IEA:UniProtKB-KW
negative regulation of filamentous growth	GO:0060258	IMSGD
negative regulation of transcription by RNA polymerase II	GO:0000122	IMSGD
nuclear-transcribed mRNA catabolic process, non-stop decay	GO:0070481	IMSGD
phosphorylation of RNA polymerase II C-terminal domain	GO:0070816	IDA:SGD
positive regulation of transcription from RNA polymerase II promoter by galactose	GO:0000435	IMSGD
protein destabilization	GO:0031648	IMSGD
protein phosphorylation	GO:0006468	IMSGD
response to oxidative stress	GO:0006979	IMSGD

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP32634
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     132.20|      39|      94|     207|     249|       2
---------------------------------------------------------------------------
  207-  249 (63.69/58.26)	EMALCRELHNKhlttLVEIFLERKCVHMVYEYAEHDLLQIIHF
  304-  342 (68.51/51.27)	DLGLARKFHNM....LQTLYTGDKVVVTIWYRAPELLLGARHY
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32634 with CDK8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) KRQINSGTNSANGSSLNGTNAKIPQFDSTQPKSSSSMDMQANTN
93
136










Molecular Recognition Features






MoRF SequenceStartStop




1) DEPSRKKNRR
2) RILAAAAAAA
3) SYQPMYQRP
546
527
11
555
536
19