<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32602

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMNNGSGRYLLTPPDDLHPYVPSSKPQEQVYPDFKPWEHTAAEDQILANFVAKGFYHTPMVNFESISARSSVHESLVTQSNILSQQFDKIIKIREDHINKIPSNSTTTLHGPGFQLPNRITLTDHRKETWLHELSSSHTSLVKIGKFIPHGLKRRQVIEQCYLKFIPLKRAIWLIKCCYFIEWKSNHKKKRSNAAGADDAISMHLLKDWTDTFVYILEKLIFDMTNHYNDSQQLRTWKRQISYFLKLLGNCYSLRLINKEIFHHWLVEFINKMENFEFLPLSLHILMIFWNDICQIDTNAPVAATITSSQKEPFFLVTKITDMLLHKYYIVSSSKSMINDENYIINDIKKNNKIKLNILKILSSLILKIFQEQSLEVFIFPTSNWEIYKPLLFEIVSNADTNQNSDMKKKLELISYRNESLKNNSSIRNVIMSASNANDFQLTIVTCKQFPKLSCIQLNCIDTQFTKLLDDNPTEFDWPTYVDQNPLTMHKIIQLILWSIHPSRQFDHYESNQLVAKLLLLRINSTDEDLHEFQIEDAIWSLVFQLAKNFSAQKRVVSYMMPSLYRLLNILITYGIIKVPTYIRKLISSGLLYLQDSNDKFVHVQLLINLKISPLMKSQYNMVLRNVMEYDVKFYEIFNFDQLVEITEQIKMRILSNDITNLQLSKTPLSIKIMVAEWYLSHLCSGILSSVNRTVLLKIFKIFCIDLEVFHHFFKWIEFIVYHQLLSDIESLEALMDILLCYQKLFSQFINDHILFTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPFVLKVDNDLRIELQSVYNDEKLKTEKLKNDKSEVLKVYSMINNSNQAVGQTWNFPEVFQVNIRFLLHNSEIIDTNTSKQFQKARNNVMLLIATNLKEYNKFMSIFLKRKDFTNKNLIQLISLKLLTFEVTQNVLGLEYIIRLLPINLENNDGSYGLFLKYHKEQFIKSNFEKILLTCYELEKKYHGNECEINYYEILLKILITYGSSPKLLATSTKIIMLLLNDSVENSSNILEDILYYSTCPSETDLNDIPLGSGQPDNDTVVTNDDKSDDDDHTVDEIDHVEYYVMMDFANLWVFQAFTCFCIKKIMENNEPAMAMEDLKNFIFQIIEITNSNDLCSQIFDQLKDMQTIEMITQIVEKDFCTSCLQNNNQKIDDNYIVVVIEIITSLSMRFQRETSGMIVISMENYHLLIKIIRQLSELNEGNLSKREIQIDAVLKIFSFHQDSIFQRIIADLSADKPTSPFIDSICKLFDKISFNLRLKLFLYEILSSLKSFAIYSSTIDAPAFHTSGKVELPKKLLNLPPFQVSSFVKETKLHSGDYGEEEDADQEESFSLNLGIGIVEIAHENEQKWLIYDKKDHKYVCTFSMEPYHFISNYNTKYTDDMATGSNDTTAFNDSCVNLSLFDARFERKNPH
Length1427
PositionKinase
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces.
Aromaticity0.11
Grand average of hydropathy-0.167
Instability index36.43
Isoelectric point6.06
Molecular weight166858.61
Publications
PubMed=7774808
PubMed=1574125
PubMed=24374639
PubMed=12200444
PubMed=14562106
PubMed=15175151
PubMed=15477388
PubMed=16076843
PubMed=16109375
PubMed=15601835

Function

Annotated function Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. 
GO - Cellular Component
mediator complex	GO:0016592	IDA:SGD

GO - Biological Function
transcription coactivator activity	GO:0003713	IDA:SGD

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IMSGD
positive regulation of transcription by galactose	GO:0000411	IMSGD
positive regulation of transcription by RNA polymerase II	GO:0045944	IMSGD









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP32602
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.49|      15|      17|     145|     161|       1
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  145-  161 (25.92/24.97)	KFIPhgLKRR.QVIEQCY
  163-  178 (28.57/18.70)	KFIP..LKRAiWLIKCCY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     961.77|     297|     349|     547|     894|       2
---------------------------------------------------------------------------
  198-  342 (115.85/60.48)	.............................................................................................................................................................................................................................DAISMHLLkdWTDTFVYILEKLIFDM.TNHYNDSQQLRTWKRQISYFLK.LLGNCYSLRLINKEIFHHwlvefiNK.MENFEFLPLSLHILMIF.............WN..DICQIDTNapvaatitssqkepfFLV..TKITDMLLHKYYIVSSSKSM....INDENY
  547-  890 (422.76/440.81)	KNFSaQKRVVSYMmpSLyRLLNILITYGIIKVpTYIRKLISSGLLYLQDSNDKFV..HVQLLINLKISPLMKSQYNM.vlrNVMEYDVKFYEIF........NFDQLVEITEQIKMRILSNDITNlqlsktplSIKIMVAEWYLShLCS..........GILSSVNRTVLLKIFKifcIDLEvfHHFFKWIEFIVYHQLLsdieslealmdillcyqklfsQFINDHIL..FTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPF.VLKVDNDLRIELQSVYND......EK.LKTEKLKNDKSEVLKVYSMINNSNQAVGQtWNfpEVFQVNIR...............FLLHNSEIIDTNTSKQFQKARNNVMlLIATNLKEY
  901- 1200 (423.16/308.45)	KDFT.NKNLIQLI..SL.KLLTFEVTQNVLGL.EYIIRLLPINLENNDGSYGLFLkyHKEQFIKSNFEKILLTCYELekkyHGNECEINYYEILlkilitygSSPKLLATSTKIIMLLLNDSVEN........SSNILEDILYYS.TCPsetdlndiplGSGQPDNDTVVTNDDK...SDDD..DHTVDEIDHVEYYVMM.....................DFANLWV...FQAFTCFCIKKIM.ENNEPAMAMED........LKNFIFqIIEITNSNDLCSQ.IFDQ......LKdMQTIEMITQIVEKDFCTSCLQNNNQKIDD............N...............YIVVVIEII.TSLSMRFQRETSG...MIVISMENY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.85|      12|      17|     383|     396|       3
---------------------------------------------------------------------------
  383-  396 (17.85/17.84)	NWEIYKPLlfEIVS
  403-  414 (19.99/11.37)	NSDMKKKL..ELIS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     133.18|      35|     860|     343|     377|       4
---------------------------------------------------------------------------
  343-  377 (54.80/31.69)	IINDIKKNNKIKLNILKILSSLILKIFQEQSLEVF
 1206- 1240 (54.13/31.20)	IIRQLSELNEGNLSKREIQIDAVLKIFSFHQDSIF
 1263- 1284 (24.25/ 9.76)	LFDKISFNLRLKLFLYEILSSL.............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     193.82|      63|     354|      63|     133|       5
---------------------------------------------------------------------------
   63-  132 (95.34/75.85)	ESISARSSVHESLVTQSN........ILSQQFDKIIKIredHINKIPSNSTTTL.HGPG.FQLPnriTLTDHRKETwLHE
  418-  490 (98.48/58.25)	ESLKNNSSIRNVIMSASNandfqltiVTCKQFPKLSCI...QLNCIDTQFTKLLdDNPTeFDWP...TYVDQNPLT.MHK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32602 with Med12 domain of Kingdom Fungi
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