<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32602

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMNNGSGRYLLTPPDDLHPYVPSSKPQEQVYPDFKPWEHTAAEDQILANFVAKGFYHTPMVNFESISARSSVHESLVTQSNILSQQFDKIIKIREDHINKIPSNSTTTLHGPGFQLPNRITLTDHRKETWLHELSSSHTSLVKIGKFIPHGLKRRQVIEQCYLKFIPLKRAIWLIKCCYFIEWKSNHKKKRSNAAGADDAISMHLLKDWTDTFVYILEKLIFDMTNHYNDSQQLRTWKRQISYFLKLLGNCYSLRLINKEIFHHWLVEFINKMENFEFLPLSLHILMIFWNDICQIDTNAPVAATITSSQKEPFFLVTKITDMLLHKYYIVSSSKSMINDENYIINDIKKNNKIKLNILKILSSLILKIFQEQSLEVFIFPTSNWEIYKPLLFEIVSNADTNQNSDMKKKLELISYRNESLKNNSSIRNVIMSASNANDFQLTIVTCKQFPKLSCIQLNCIDTQFTKLLDDNPTEFDWPTYVDQNPLTMHKIIQLILWSIHPSRQFDHYESNQLVAKLLLLRINSTDEDLHEFQIEDAIWSLVFQLAKNFSAQKRVVSYMMPSLYRLLNILITYGIIKVPTYIRKLISSGLLYLQDSNDKFVHVQLLINLKISPLMKSQYNMVLRNVMEYDVKFYEIFNFDQLVEITEQIKMRILSNDITNLQLSKTPLSIKIMVAEWYLSHLCSGILSSVNRTVLLKIFKIFCIDLEVFHHFFKWIEFIVYHQLLSDIESLEALMDILLCYQKLFSQFINDHILFTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPFVLKVDNDLRIELQSVYNDEKLKTEKLKNDKSEVLKVYSMINNSNQAVGQTWNFPEVFQVNIRFLLHNSEIIDTNTSKQFQKARNNVMLLIATNLKEYNKFMSIFLKRKDFTNKNLIQLISLKLLTFEVTQNVLGLEYIIRLLPINLENNDGSYGLFLKYHKEQFIKSNFEKILLTCYELEKKYHGNECEINYYEILLKILITYGSSPKLLATSTKIIMLLLNDSVENSSNILEDILYYSTCPSETDLNDIPLGSGQPDNDTVVTNDDKSDDDDHTVDEIDHVEYYVMMDFANLWVFQAFTCFCIKKIMENNEPAMAMEDLKNFIFQIIEITNSNDLCSQIFDQLKDMQTIEMITQIVEKDFCTSCLQNNNQKIDDNYIVVVIEIITSLSMRFQRETSGMIVISMENYHLLIKIIRQLSELNEGNLSKREIQIDAVLKIFSFHQDSIFQRIIADLSADKPTSPFIDSICKLFDKISFNLRLKLFLYEILSSLKSFAIYSSTIDAPAFHTSGKVELPKKLLNLPPFQVSSFVKETKLHSGDYGEEEDADQEESFSLNLGIGIVEIAHENEQKWLIYDKKDHKYVCTFSMEPYHFISNYNTKYTDDMATGSNDTTAFNDSCVNLSLFDARFERKNPH
Length1427
PositionKinase
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces.
Aromaticity0.11
Grand average of hydropathy-0.167
Instability index36.43
Isoelectric point6.06
Molecular weight166858.61
Publications
PubMed=7774808
PubMed=1574125
PubMed=24374639
PubMed=12200444
PubMed=14562106
PubMed=15175151
PubMed=15477388
PubMed=16076843
PubMed=16109375
PubMed=15601835

Function

Annotated function Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. 
ECO:0000269	PubMed:12200444
ECO:0000269	PubMed:15601835
ECO:0000269	PubMed:16076843
ECO:0000269	PubMed:16109375
GO - Cellular Component
mediator complex	GO:0016592	IDA:SGD
GO - Biological Function
transcription coactivator activity	GO:0003713	IDA:SGD
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IMSGD
positive regulation of transcription by galactose	GO:0000411	IMSGD
positive regulation of transcription by RNA polymerase II	GO:0045944	IMSGD

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP32602
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.49|      15|      17|     145|     161|       1
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  145-  161 (25.92/24.97)	KFIPhgLKRR.QVIEQCY
  163-  178 (28.57/18.70)	KFIP..LKRAiWLIKCCY
---------------------------------------------------------------------------
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     961.77|     297|     349|     547|     894|       2
---------------------------------------------------------------------------
  198-  342 (115.85/60.48)	.............................................................................................................................................................................................................................DAISMHLLkdWTDTFVYILEKLIFDM.TNHYNDSQQLRTWKRQISYFLK.LLGNCYSLRLINKEIFHHwlvefiNK.MENFEFLPLSLHILMIF.............WN..DICQIDTNapvaatitssqkepfFLV..TKITDMLLHKYYIVSSSKSM....INDENY
  547-  890 (422.76/440.81)	KNFSaQKRVVSYMmpSLyRLLNILITYGIIKVpTYIRKLISSGLLYLQDSNDKFV..HVQLLINLKISPLMKSQYNM.vlrNVMEYDVKFYEIF........NFDQLVEITEQIKMRILSNDITNlqlsktplSIKIMVAEWYLShLCS..........GILSSVNRTVLLKIFKifcIDLEvfHHFFKWIEFIVYHQLLsdieslealmdillcyqklfsQFINDHIL..FTKTFIFIYKKVLKEKDVPAYNVTSFMPFWKFFMKNFPF.VLKVDNDLRIELQSVYND......EK.LKTEKLKNDKSEVLKVYSMINNSNQAVGQtWNfpEVFQVNIR...............FLLHNSEIIDTNTSKQFQKARNNVMlLIATNLKEY
  901- 1200 (423.16/308.45)	KDFT.NKNLIQLI..SL.KLLTFEVTQNVLGL.EYIIRLLPINLENNDGSYGLFLkyHKEQFIKSNFEKILLTCYELekkyHGNECEINYYEILlkilitygSSPKLLATSTKIIMLLLNDSVEN........SSNILEDILYYS.TCPsetdlndiplGSGQPDNDTVVTNDDK...SDDD..DHTVDEIDHVEYYVMM.....................DFANLWV...FQAFTCFCIKKIM.ENNEPAMAMED........LKNFIFqIIEITNSNDLCSQ.IFDQ......LKdMQTIEMITQIVEKDFCTSCLQNNNQKIDD............N...............YIVVVIEII.TSLSMRFQRETSG...MIVISMENY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.85|      12|      17|     383|     396|       3
---------------------------------------------------------------------------
  383-  396 (17.85/17.84)	NWEIYKPLlfEIVS
  403-  414 (19.99/11.37)	NSDMKKKL..ELIS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     133.18|      35|     860|     343|     377|       4
---------------------------------------------------------------------------
  343-  377 (54.80/31.69)	IINDIKKNNKIKLNILKILSSLILKIFQEQSLEVF
 1206- 1240 (54.13/31.20)	IIRQLSELNEGNLSKREIQIDAVLKIFSFHQDSIF
 1263- 1284 (24.25/ 9.76)	LFDKISFNLRLKLFLYEILSSL.............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     193.82|      63|     354|      63|     133|       5
---------------------------------------------------------------------------
   63-  132 (95.34/75.85)	ESISARSSVHESLVTQSN........ILSQQFDKIIKIredHINKIPSNSTTTL.HGPG.FQLPnriTLTDHRKETwLHE
  418-  490 (98.48/58.25)	ESLKNNSSIRNVIMSASNandfqltiVTCKQFPKLSCI...QLNCIDTQFTKLLdDNPTeFDWP...TYVDQNPLT.MHK
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32602 with Med12 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




NANANA









Molecular Recognition Features






MoRF SequenceStartStop




1) SGRYLLTP
5
12