<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32600

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMLRSGAASASSGGGRGPSSSLSSVKRSKHDDTRLPEAYQQERPPWRPPLHPTAARRHVPDLFPTKSGQPEDDMSQISVRKGFAPRTYVHNEYFCAHDAIHTRLLSPDALDTLSDWMDEVMQRRRELDRASIATNQYKPPSRVTLNDAKLANYVKDLADPTVPLMRLSRSVPHGFRGEKLFEMLWVGGALPSTATAATTTVNYFHSRIAGPPSASSASASASASASTSAPRKSVDIPRAVWFIRALGAAELSSLRNKSAATIIPEITSNLCAWMAKQVAELNLVHTAESNSTATASPAPVSPSTHLPRTPSAAPSSLSRSVAAAHFARTPSNLNPASSPSLAPAKEVCYVLQNDADEERWIAKWSYSLSLARHLHAQNLLDRSVLVRWIVDAFSASNLVQLPFLLELVQEVLLLVLRRRCFVKPFLTALLVQIPNVDARLDPSSSGGLRTKLVLLFRIVCENSPESLISPRLWFEHSASLTQLLNELNAETPLPLSHQAFEFVEQTVRPRVDRLLLRPHSGNTTQNASLETQETLSSPLQHHLDIQALDSFDMTTIDRVFLQNTSATHRYAKHDRDDTVWATRIDTILTWACTDRRSGVVRQYLAATLIEKIRFGVPWEESSEETDQVSLPAFDLEHNTTKTRKINVEPMLIKWLGDVETSLNQKLDESSTQTRVSLLATVDVGAIVVLLGELARRGVFSYTKYLQRLIARGMTAPSASSATEQHQNPDGSVQTPSSVSASRDSLHLRLLRSLPLYDQPASVYQQRRQAIYGDRTKETYEDAAQRRALRQLQSFLPFAFAQEVQAEASRIAVSPSPSTPDPVHALSRLWSASRFVRCRLFRSELLPAVTARVERLNGDQLSQISAVLVMADDFEGLAQLLATLLLRPLSDNLARAAFNMVIEYSLVWRSMDIMATLNQLVRQQLNHSSSIRNDRQAVMASTTLLRLRTLIENVPSRISASALDPGADHDALQQHVQALRPNVDALLDRFAKLTRSSVESDGSIASSSTPVEGSDMLTPFRSLFVQPDAMSDEMIERTIVDSVFARSDTSLVPSAVRLIDQLYLEASLEIDERHARWLSNLVLSIEQGTHSDASMKALLSLLTRLIAHGTLNLQLAMDTFLLPYLLSSVRRLTDPATRNTEVPMHIGSIVSSLGEIFASSLGETVPATSYEDMRAFGAQTKLLFAQVNLPSLLRAATCLISAFDETSTVPASEKAQIESFCNALLQSQPMQIAFRQEPRLCILAIKDTCKSMWGCNPSRVIDLAMACLDPTSLLLRDVGTIDAASVKNRLDGWDTAVVATELVEIFERLQLVESSFQTRADSKTKALASGIFDDLFVQLPDIGAQLVRDCQSSGLVSRFTDIGIKILSDKVKAASTDADKTPRRMDNSHKGMTNFGPDDGADAPAQAKTAADAAKHGPCVGEQQVESTLRSVVRMCEQQHSFIFNATDADSCAQLLLHVVTSLEDALGSMAAASHQDKVARLVTVLHGSMTFQLMHLVLRFGCLWNGTMRALALRLVKALLELTRQAGEKTECGGSFALLLDSLSFVLDELPPTLLSTCLPELETQLQTIELATQERSDKLQQLSFRLDNATTANAWLVASSAGAALKKGWFTSGGLNPWECAEYLEAPPAANPAAAGAAGAAGAAAGGNARAGVVETGRSGVNSRAAVESPGAVTVNDAETCNSLPGARTGWTGKLWLNTAIPLSMLGVRVTRDLVPNYASPASPGGVSVGSNDASSQPWTTGGGASAIGVGTCSAMDMDAKEALAALPMFVESERTYGERSAGEPAYSRDLRRGLLVEPNLPCRTRDERGETSQEPARATMDPAGAHTSVTSSATHDPSHVIDLTWDADEEMPLSTHPHHPAVATTSRKRRLSKRD
Length1876
PositionKinase
OrganismUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Ustilaginomycotina> Ustilaginomycetes> Ustilaginales> Ustilaginaceae> Ustilago.
Aromaticity0.06
Grand average of hydropathy-0.173
Instability index48.14
Isoelectric point6.23
Molecular weight204263.12
Publications
PubMed=17080091

Function

Annotated function Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex (By similarity). 
ECO:0000250
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP32600
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     906.42|     333|     367|     415|     780|       1
---------------------------------------------------------------------------
  415-  778 (495.19/366.16)	LRR.RCFVkPFLTALLVQIPNVDARLDPSSSGGlrTKLVLLFRIVcenSPESLISPRLW.FEHSASLTQLLNELNAETPLPLS.........HQAFEFVEQTVRPRVDRLLLRPHSGNTTQNASLETQETLSS...PLQ........HHLDIQAlDS.....FDMTTIDRVFlqntsathryAKHDRDDTVWATR.IDTILTWAC..TDRRSGvvrQYLaATLIEKIRFGVPWEESSEETdqVSLPAFDLEHNTTKTrKINVEPMLIKWLgdvETSLNQKLDESSTQTRVSL.LATV..DVGAIVV.LLGE....LARRGVFSYTKYLQRLIARgMTAPS.ASSATEQHQNPDGSVQTPSSVSASRDSLHLRLLRSLPLydQPAsVYQQRRQAIYG..DRTKETY
  787-  895 (61.30/21.65)	LRQlQSFL.PFAFAQEVQAEASRIAVSPSPSTP..DPVHALSRLW...SASRFVRCRLFrSELLPAVTARVERLNGDQLSQIS....avlvmADDFEGLAQLLAT....LLLRPLSDNLARAA..........................................................................................................................................................................................................................................................................................
  944- 1251 (349.92/197.86)	.....................................................................RLRTLIENVPSRISasaldpgadHDALQQHVQALRPNVDALLDRFAK...LTRSSVESDGSIASsstPVEgsdmltpfRSLFVQP.DAmsdemIERTIVDSVF..........ARSDTSLVPSAVRlIDQLYLEASleIDERHA...RWL.SNLVLSIEQGTHSDASMKAL..LSLLTRLIAHGTLNL.QLAMDTFLLPYL...LSSVRRLTDPATRNTEVPMhIGSIvsSLGEIFAsSLGEtvpaTSYEDMRAFGAQTKLLFAQ.VNLPSlLRAATCLISAFDETSTVPASEKAQIESFCNALLQSQPM..QIA.FRQEPRLCILAikDTCKSMW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     165.38|      51|    1107|     158|     252|       2
---------------------------------------------------------------------------
  181-  232 (82.85/58.51)	EMLWVGGALP.STATAATTTVNYfHSRIAGPPSASSASASASASASTSAPRKS
  279-  330 (82.53/49.02)	ELNLVHTAESnSTATASPAPVSP.STHLPRTPSAAPSSLSRSVAAAHFARTPS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.30|      24|     581|    1254|    1292|       3
---------------------------------------------------------------------------
 1254- 1277 (41.30/43.61)	NPSRVIDLAMACLDPTSLLLRDVG
 1318- 1341 (39.99/14.45)	ADSKTKALASGIFDDLFVQLPDIG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.24|      13|    1530|     139|     151|       7
---------------------------------------------------------------------------
  139-  151 (23.13/10.83)	PSRVTLNDAKLAN
 1670- 1682 (25.11/12.27)	PGAVTVNDAETCN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      33.22|       9|      14|    1785|    1794|       8
---------------------------------------------------------------------------
 1785- 1794 (13.89/11.98)	PAYSRDlRRG
 1802- 1810 (19.33/11.48)	PCRTRD.ERG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32600 with Med12 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MFVESERTYGERSAGEPAYSRDLRRGLLVEPNLPCRTRDERGETSQEPARATMDPAGAHTSVTSSATHDPSHVIDLTWDADEEMPLSTHPHHPAVATTSRKRRLSKRD
2) MLRSGAASASSGGGRGPSSSLSSVKRSKHDDTRLPEAYQQERPPWRPPLHPTAARRHVPDLFPTKSGQPEDDMSQISVRK
3) NYASPASPGGVSVGSNDASSQPWTTGGGASAI
4) STDADKTPRRMDNSHKGMTNFGPDDGADAPAQAKTAADAAKHG
1769
1
1717
1373
1876
80
1748
1415










Molecular Recognition Features






MoRF SequenceStartStop




1) DLTWDADE
2) LPEAY
3) MLRSGAASA
4) SLSSVKRSKH
5) TSRKRRLSK
1843
34
1
20
1866
1850
38
9
29
1874