<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32596

Description Mediator of RNA polymerase II transcription subunit 12
SequenceMTSRPPLGVQQRQPQQRKLSGPVLSQQRSLSQSQAQAQAQAQQQQQQQQQQQQQQQQQQQQQQQQQYDASSYLPPPPPVPTRNNNHNNFSNPNNTLNNAFPPELAQQDPTSIVSPADPSGSSPALSVGVGRYGTQRRGGSRLRLELSHNDPFDAFSSPTVIESPGLVEPHTQNGSLTHPMMPLADGPDLGDMSPHHRVSIPPQHLDADVPIPFPQRRPKAVPDHSRREQPPPPPNPARKDTRPKPYTIELPAAAPRYRIRGRSDGQGGQGRSSALATASSSSTTGSSATANYGCADFFPWSGTAGNHPEDQFNDTAIRVGYSEKQFVTPETQSAKTVLFHGLKQRSGLNALSIFFAGVLWHRRNAGQVTAPSTFKPPPRVTLTDTKKEVWLKDLANSAIPLRKLSRSIPHGVRNKVLLDQCLNKNVPIDRAVWLAKCVGTNELRTFRRLGKGNNASVLEGERKWLKDWTLVIENFIERVFFSFGEQNWKAKVNYAIQLAAHLYAEGLMDREHYLDWTVSSLESSHHSQLPMWIIIAQIYWKDLLRLRKYGRRLATALISHYHVIHAHEDRDIYVPLLSKLSHLLGTLMVATPENFVSPSVWVKYRDALKTCLPQGDEARHRSFAGINYRNEQLVASANRSQPAARIILVRNLDQVILEKPMPDEFPAQCWTVSKDKAALVRALLEWCTTPYRPGLAKVYVAHRILMHWSTLRLDVTSAVLGFMEGEALEEMECKDALYHLVSELVRSGIFLVSQYIQWLITRGGLTDPQLVMPDGPPFSRLLAELPSHILGSSQKRSRDALLRRAGFRIADDAQDADMAIRHLRHALGLPAGVTDAVSMGRPFPIKKIARRIEDASRARKAEIASWLRNTVFGGELEHSSLNAFGQHDLSTRLFNSIRTVFEAAQEFSVFADVLRMLTKCSNPEVLASIADTITRYTFVFAALSCLKNLFSILHSRLREVQEQGIGARPLLASLAYLASRVPGMDEVAMQLKSDLAVSDRHNPVDACSPVSDNMVSRLSDHDGDLHEEVEKLLANGTSLDRNTMDRLFSAVIQRLQAYWSKGDEQQRVYGILLKRLRMFNPQHFDELMTRWLLYVRGLDTRATILHLYPLLVSIGCLEMPAILATASESPNALGAGNSRHPQSAVATSQIVQTTFRTRYMQEVLQLFMAPVSSESLLTPEERYRFGILQEQAKKESPRELLCLIQLALAEYTCARAQNDLEELPLDKEANQDRLLRLLKLLVLKDPVGVAKALQIRGPDAQIDGWIDRMTTKLLVTAAGEGTRVTFEQVLNLTNEFTLPFCHVKLLLSLSSNDQQQNAADSGERVPSNVELFMNAMEKSMDAQRVSWIGMLPSLSPDLTHHLKNQAQARFLNLLPSARNPPSMTLDRSMLQMRLQKAENFLAIMDTIIRGSGPMGFRQHQLVPVMVERFTDILELLQTLTPFPSQAGGLGGWGGQGASAAAAGGDSHIDLKSDILNHWLPLFLNFLTLHAQTFDTSKPGNEVRGRALMVCAGLIRELDLIHGPDFDTRQLGGRIFDLACVLVDNLAEDARLQCIQALKSPSDVKLRYIFSYQDNPHANLMLCHKERPTAMAIGTATGTGSPAPGTTPTHTPGVTPGPQNAVGAGSAVSGAGGSGNGGSYFSLPGHTQQAQAQGWHAAQQQQQQQQQQQSQQQQQQQQQQQYQLQQQQQQGGTGPIPSPAISINTNLTHLNLGNNSNPPTPSPMNPTRPAMMMVANPWGGYSWVPTMGGPQERLTPFNFRQWEMLSEPTSQVGENDTAVNLMLFESRKVQ
Length1789
PositionKinase
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Sordariales> Sordariaceae> Neurospora.
Aromaticity0.07
Grand average of hydropathy-0.398
Instability index51.18
Isoelectric point8.83
Molecular weight198737.51
Publications
PubMed=12655011
PubMed=12712197

Function

Annotated function Component of the srb8-11 complex. The srb8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The srb8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex (By similarity). 
ECO:0000250	
	a target='_blank' href='https://www.uniprot.org/unirule/RU364149' style='color:#FF0000;'>RuleBase:RU364149
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP32596
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     141.08|      15|      15|      36|      50|       1
---------------------------------------------------------------------------
   34-   49 (27.45/10.70)	QaQAQAQAQQQQQQQQ
   50-   65 (29.97/12.32)	QqQQQQQQQQQQQQQQ
 1647- 1661 (27.89/10.98)	Q.QAQAQGWHAAQQQQ
 1662- 1676 (32.58/14.00)	Q.QQQQQQSQQQQQQQ
 1677- 1689 (23.18/ 7.96)	Q.QQQYQLQQQQQQ..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.25|      16|      18|     337|     354|       2
---------------------------------------------------------------------------
  337-  354 (23.69/17.43)	VLFHglKQRSG.LNALSIF
  358-  374 (26.56/13.08)	VLWH..RRNAGqVTAPSTF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.80|      12|      15|     408|     421|       3
---------------------------------------------------------------------------
  408-  421 (16.66/14.99)	IPhgVRNKVLLDQC
  426-  437 (23.13/12.43)	VP..IDRAVWLAKC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.35|      11|      15|       5|      16|       4
---------------------------------------------------------------------------
    5-   16 (18.56/14.11)	PPLGvQQRQPQQ
   22-   32 (19.79/ 9.74)	PVLS.QQRSLSQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      90.62|      21|      21|     202|     222|       5
---------------------------------------------------------------------------
  202-  222 (37.30/17.08)	PQHLDADVPIPFPQRRPKAVP
  224-  242 (32.29/13.73)	..HSRREQPPPPPNPARKDTR
  243-  258 (21.03/ 6.18)	PKPYTIELPAAAPRYR.....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      63.87|      11|      16|    1716|    1726|       6
---------------------------------------------------------------------------
  100-  110 (18.05/ 6.02)	FPPELAQQDPT
 1716- 1726 (24.22/11.13)	NPPTPSPMNPT
 1735- 1745 (21.60/ 8.96)	NPWGGYSWVPT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.88|      15|      15|    1587|    1601|       7
---------------------------------------------------------------------------
 1587- 1601 (27.23/13.68)	PTAMAIGTATGTGSP
 1603- 1617 (27.65/14.01)	PGTTPTHTPGVTPGP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.60|      18|      21|     669|     688|       8
---------------------------------------------------------------------------
  671-  688 (31.39/24.21)	TVSKDKAALV....RALLEWCT
  689-  710 (29.21/12.70)	TPYRPGLAKVyvahRILMHWST
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      64.75|      20|      20|    1045|    1064|       9
---------------------------------------------------------------------------
 1045- 1064 (34.14/16.50)	DRLFSAVIQRLQAYWSKG.DE
 1066- 1086 (30.60/14.13)	QRVYGILLKRLRMFNPQHfDE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.83|      15|      17|     768|     782|      11
---------------------------------------------------------------------------
  768-  782 (28.81/18.06)	PQLVMPDGPPFSR..LL
  786-  802 (21.02/10.73)	PSHILGSSQKRSRdaLL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.33|      20|      22|     980|    1001|      12
---------------------------------------------------------------------------
  980-  999 (32.83/25.55)	RVPGMDEVAMQLKSDLAVSD
 1003- 1022 (35.50/19.67)	PVDACSPVSDNMVSRLSDHD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      63.73|      19|      21|    1159|    1179|      13
---------------------------------------------------------------------------
 1159- 1177 (31.80/22.48)	YMQEVLQLFMAPVSSESLL
 1183- 1201 (31.93/14.92)	YRFGILQEQAKKESPRELL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.58|      16|      21|    1499|    1519|      15
---------------------------------------------------------------------------
 1499- 1517 (21.58/21.89)	GNEVRGRALmvcAGLIREL
 1522- 1537 (30.00/12.53)	GPDFDTRQL...GGRIFDL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32596 with Med12 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MTSRPPLGVQQRQPQQRKLSGPVLSQQRSLSQSQAQAQAQAQQQQQQQQQQQQQQQQQQQQQQQQQYDASSYLPPPPPVPTRNNNHNNFSNPNNTLNNAFPPELAQQDPTSIVSPADPSGSSPALSVGVGRYGTQRRGGSRLRLELSHNDPFDAFSSPTVIESPGLVEPHTQNGSLTHPMMPLADGPDLGDMSPHHRVSIPPQHLDADVPIPFPQRRPKAVPDHSRREQPPPPPNPARKDTRPKPYTIELPAAAPRYRIRGRSDGQGGQGRSSALATASSSSTTGS
2) TAMAIGTATGTGSPAPGTTPTHTPGVTPGPQNAVGAGSAVSGAGGSGNGGSYFSLPGHTQQAQAQGWHAAQQQQQQQQQQQSQQQQQQQQQQQYQLQQQQQQGGTGPIPSPAISINTNLTHLNLGNNSNPPTPSPMNPTRPAMMMVANPWGGYSWVPTMGGPQERLTPFNFRQWEMLSEPTSQVGENDTAVNLMLFE
1
1588
286
1784










Molecular Recognition Features






MoRF SequenceStartStop




1) RKLSGPVLS
17
25