<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32249

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDDILNEQFGRVEKALTTLVDSIAAYNPATQAAIDLVAADDQLSEGLDQLAKHQANHARIQTLRAEADALEEQLKTSVKKLAELRRELFATPATTFPHDSRAVPFDELLQYATNISRYTVPPTYRERVPTTEKDKDEEDGISSGAPTNGVNTPAIVPDAPDAPNEGPEAQKGAEDADGAAPDITAEEEEWLKKLKASNLAWYPWPSTEKIRNGILYRLSYWREKGNDLDEFNIPAYLEEQRKKALGDEEKSEEAPEQATEEQPHRETARPTMPLSAFTGLDDMDEM
Length286
PositionMiddle
OrganismSetosphaeria turcica (strain 28A) (Northern leaf blight fungus) (Exserohilum turcicum)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Pleosporomycetidae> Pleosporales> Pleosporineae> Pleosporaceae> Exserohilum.
Aromaticity0.06
Grand average of hydropathy-0.803
Instability index45.64
Isoelectric point4.48
Molecular weight31850.70
Publications
PubMed=23236275
PubMed=23357949

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP32249
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      89.55|      26|      26|     128|     153|       1
---------------------------------------------------------------------------
  128-  153 (44.33/19.52)	VPTTEKDKDEEDGISSGAPTNGVNTP
  156-  181 (45.21/20.02)	VPDAPDAPNEGPEAQKGAEDADGAAP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.90|      24|      26|      36|      59|       2
---------------------------------------------------------------------------
   36-   59 (39.94/25.07)	LVAADDQLSEGLDQLAKHQANHAR
   63-   86 (36.97/22.74)	LRAEADALEEQLKTSVKKLAELRR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.25|      15|      21|      87|     101|       3
---------------------------------------------------------------------------
   87-  101 (28.68/21.07)	EL..FATPAT..TFPHDSR
  107-  125 (18.57/11.10)	ELlqYATNISryTVPPTYR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32249 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DEFNIPAYLEEQRKKALGDEEKSEEAPEQATEEQPHRETARPTMPLSAFTGLDDM
2) VPPTYRERVPTTEKDKDEEDGISSGAPTNGVNTPAIVPDAPDAPNEGPEAQKGAEDADGAAPDITAEEEE
229
120
283
189










Molecular Recognition Features






MoRF SequenceStartStop




1) KIRNGILYRLSYWREK
209
224