<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32144

Description Mediator of RNA polymerase II transcription subunit 16
SequenceMSGHDIPIQLLDGGSIDVQHHDQMNAMDTAMNLDDVDVDLFGDSVMVDTALDGLSAPRPMPSKHLRQRLDDLRTHGCHQAIAWSRQGTIASITKDGRSVEFRFLRCRPEDAGWELSEPYLYGPAIIPMHTTAGPIVHLAWSTTTPTPELAVVDAVGRITILSLTIHLNRPFITRKWDADPVDDLHAVVGCYWLPLLNPRQLSVMYGPAVWVQPEDAKTPGDGLYKYESSYTKPTGPNHPNTNKSALVSITTNGLLRLMFQNNNTRLEETAIELESITSSDDLITHAALCNDKNALLIALATASKQLRIVKATISWGNDKPAEKQAHPQNAAITASISEQHVAVAPLLQHDSNDAVLDDSMSQLSHIEFLPSIPKDKNLQAHHNPVVLTVRSHLPAEGASLYEQEPTSVIDRWEVVSEQPQTLHPAFAQLNTGNGMTNPNSMTRLRKLDAVVIPKIVVSVQVMYLGKVVCFAFSDGTIQYRDRVTMMELFNEQNVHRVLSPHDVGFQYVNDTPCLQVAFSPTNCSYAQISEDWDIKWNSMRYTLTDASTALQSAQQSAVMAALTLALSSAVTTSINFDDILALARPYSDNPQFAASWVKDLVNLLKLNFDFSDEGHYDSLVRSPLFQISLSILNHMGFRGEFRPRPFEGKLSFLILQMRNFIVLVSLSLNAPKSLGISPLDEAEVVEVLVGCVTWAHTLIGWMVDCLFDLADNPAFISILKEQKRFPDLANFLRARGDVSLHLLCCSSARGFIMAACRRLAHLEGMSNRAIRYWEASRLKTENNDGSGGGGKVLPDSLYFAYRKMQRATSSSLIKVQELNEVLQGFAQDVRTAYQASLSRRQSGNNQPQQQHPSPQQNKNQPQQQQNAEDQFIKKAQAHCELDMLLGANPPPCFREVLLKFFTQTLPVFKSHVDPAKLYFANYEVLEIEENPRILAARKARRKYIDVFTKREIQFPTSSGDARGGGGGSGGGSAATATAAAAAAAAAAAGGEDDGKRGGITGTGEGQTPSQSTLAPGGGISIKIELGTGSTPNLKGVSTPAGGGGGGSSGNAKTAGGNNAAAVGVNAANGGVGGGGGGGGNTNNNGTAENTSNQWRRCVRCTAVMEDFPGNHYRAGFTFVMAQQRKCACGAWWALVPKTTGGEGATEGW
Length1148
PositionTail
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Sordariales> Sordariaceae> Neurospora.
Aromaticity0.07
Grand average of hydropathy-0.253
Instability index39.51
Isoelectric point6.14
Molecular weight124549.23
Publications
PubMed=12712197

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364149
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central
GO - Biological Function
GO - Biological Process
positive regulation of transcription, DNA-templated	GO:0045893	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP32144
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      85.74|      15|      24|     961|     975|       1
---------------------------------------------------------------------------
  961-  975 (30.88/11.01)	ARGGGGGSGGGSAAT
 1040- 1053 (26.53/ 8.63)	AGGGGGGS.SGNAKT
 1067- 1081 (28.33/ 9.62)	ANGGVGGGGGGGGNT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      89.49|      29|      96|     596|     640|       2
---------------------------------------------------------------------------
  592-  621 (45.23/25.07)	FAASWVKDLVNlLKLNFDFSDEGHYDSLVR
  637-  665 (44.25/50.29)	FRGEFRPRPFE.GKLSFLILQMRNFIVLVS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      99.22|      29|      86|     120|     148|       3
---------------------------------------------------------------------------
  120-  148 (56.62/32.48)	LYGPA..IIP..MHTTAGPIVHLAWSTTTPT.PE
  204-  237 (42.60/22.68)	MYGPAvwVQPedAKTPGDGLYKYESSYTKPTgPN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     128.17|      45|     216|      70|     117|       4
---------------------------------------------------------------------------
   70-  117 (79.53/58.58)	DDLRTHG..CHQ......AIA.WSRQGTI..ASIT.KDGRSVEfrfLRCRPEDAG..WELSE
  280-  338 (48.64/27.68)	DDLITHAalCNDknalliALAtASKQLRIvkATISwGNDKPAE...KQAHPQNAAitASISE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.92|      17|      43|     463|     479|       6
---------------------------------------------------------------------------
  463-  479 (32.18/18.63)	YLGKVVCF..AFSDGTIQY
  507-  525 (29.73/16.70)	YVNDTPCLqvAFSPTNCSY
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32144 with Med16 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GGEDDGKRGGITGTGEGQTPSQSTLAPGGGISIKIEL
2) SLSRRQSGNNQPQQQHPSPQQNKNQPQQQQNAEDQFI
3) TGSTPNLKGVSTPAGGGGGGSSGNAKTAGGNNAAAVGVNAANGGVGGGGGGGGNTNNNGTAENTSN
989
836
1027
1025
872
1092










Molecular Recognition Features






MoRF SequenceStartStop




1) AAAAAAA
979
985