<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32134

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMSASGTAAVSGGSLPALLMANGGGGGGSGGPGGGPGGGKGIGKPDIKPPGGGGPSAGGTGTAGKVLLGGDKHETWQMELLMERLRTKAKSAQYKSFQEMSKSVRMSLLEKRYALDAVEKSNLQKTLDSMQYCIKVTTRQGLVERLDCLTRQLGLKLSEDTSGLFISSDMFYLEIILDPAGGTVQDVKVHHECKMKQQSCSELVACLQRGDFADFTTQLEGLASIYQLNAEKKIKVNAFVALQALETDLHTLYTLSLQHYADVHAQLHKAPLGVVQKRRGGHPMRLTYFVAPYELLDLTTRTMNVLSAELIAQERIGCSVAVVLEASSANKLQIQPLLVVNAGSGSAASVLQPVYTPIEKHNSTSLPATFVLRLSKPMPLNSAMMRAIRAIVGGSGAGEQQQAGEGSSGGGGLPGSLLGLIAHHASAGSVTDLAKGLLVSLPDQYHCYFLTDNPSLRGMMVSSIPFTEPQHVPKILTYLRQQAVFNQLLSSCIRTNAPGGERNDRRLLVFEVSALSCQNITVSLIHPYEESLATVEFDLQNIANIAVRIYCSEGETSDEHTVERISAVLQRCMSIPVTLRALMKWEEEHEQKFKPNVLGSSTVSSGGAGGGPGGGGGGGGGAGGATPGLNNGGDGTGPAGGGLTGSGDDGTMNGFNLSSLAKKRRMEDFCKSPKSDSNLSVGGGGGGGASDAVGTEKANTGAAGVAGATGATSSSSTTTTTTITSQDVGAAASGATGAGNSSEQRLQTGGATEEDTGGLPLFGETVPAAAGTDAKGSSLGGTTTTNAMISAKNEPPSPALGAVSPIASKVVSPSGGAGLEKTPLPTISGSGLEIIPLATSAGTVAASGFPSPSVTSAPIAVPQSNATGSTSSTTATSSSTVKKSSSSSGGGSGSHRKTSDSSERTSSSKSEKKKKRKTDDSAMGPPNKLPYKSSGESSPHRSGGGVGGSSLSTSKSSPKHSSPVHHSSPKHSGSGGPLGFHSPKHGSSVSPKHPLHSGGSSTGGGKPSMSALKSAAASGSSPGSKSSDVGGGGGGTSGASISTAGSSSKERDKERSEKKALHAAGSSSSSSSGGRSKNSSLKLKQLDLAAGSSMSISMAAGIGDAGSSSPVAPMIDLTGPVPGAAAALGGLGLPGPGMTTASSMLQALQAAKNRKTPTAAGGSTGLGGSLSAVIDKLKSAQSADDEAVLILPEMTITQQTTSLAAGTGGIGTGVNIGGFGGLKDSAKSGSGLLPSGGTSSLANCVSSTAGIAGAGKNKSVLSSSNSSFNPCFSLTHLSALTQSSSATSAPSLTAALTSGKASDGAFPSSTKSPHYTSSTSGSSKAPFQKSNSFGSLGGSGGTGLKASKVSSPKSTSNPASSSSSSAYSSKEKGGSTSGRSKSSSASSLTGAMLTGATGGTGSGTGSSSSALVNPISIMKMLGYPTGSMGNMEGFAKSLDTKFQIPKLSARSNSSATGTASTGSSESGEAGSKSAKDPAARKQKQQTPVTTAAPSVGSSPVRQLSSTSPSGEASARLLDGSLQTKSNSSDGSMFPTAGRASPGMLSGSASRDGFRSGGGSGSAPGTPTTTSSMVLPPFPSPISGGASNDGDSGTLMRPPSRPSSTISNHSHSSQDGIGGGSGNSGDAVAGSTPAVASMASMMMMNAMSAAAGGGHTQQLIAALAAKSGVLPPPPPNNLLNNHHSNHLNNHVVGGAAALNNLPSPASVSVHLVKSPAPSPLPFASPHSNASSTHAAGAAGLDDDLMDEALIGIGSK
Length1753
PositionMiddle
OrganismAnopheles gambiae (African malaria mosquito)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Culicoidea> Culicidae> Anophelinae> Anopheles.
Aromaticity0.03
Grand average of hydropathy-0.261
Instability index51.19
Isoelectric point9.29
Molecular weight173757.45
Publications
PubMed=12364791
PubMed=14747013
PubMed=17210077

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP32134
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             9|     520.99|      65|      65|     949|    1013|       1
---------------------------------------------------------------------------
  599-  674 (58.97/11.27)	SST......V..........SSGG.AGGG..............pGGGGGGGGGAGGA........T..........P...G............L......N.............NGGdgtGPAGGGLTgS..GDdgtmnGFNlS.........SLAKK.....RrmedfcKS....P.KS
  675-  756 (50.34/ 8.27)	DSN......L.........SVGGG.GGGGasdavgtekantgaaGVAGATGATSSSST.......T..........T...TTT..........ItsqdvgA.............AAS...GATGAGNS.S..EQ.......R.L.........QTGGA.....T......EE....D.TG
  877-  931 (59.73/11.54)	SST......V.KK......SS....SSSG...............GGSGSHRKTSDSS...........................................E.............RTS...SSKSEKKK.K..RK.....TDD.S.........AMGPP.....N......KL....P.YK
  960- 1024 (97.49/24.67)	SSP......V.HH......SSPKH.SGSG...............GPLGFHSPKHGSSV.......S..........P...KHP..........L......H.............SGG...SSTGGGKP.S..MS.....ALK.S........aA..AS.....G......SS....PgSK
 1025- 1106 (45.28/ 6.51)	SSD......V.............G.GGGG...............GTSGASISTAGSSSkerdkerS..........E...KKA..........L......H.............AAGsssSSSSGGRS.K..NS.....SLK.LkqldlaagsSMSIS.....M......AAgigdA.GS
 1107- 1185 (55.48/10.06)	SSP......V.APmidltgPVPGA.AAAL...............GGLGLPGPGMTTAS.......SmlqalqaaknR...KTP..........T......A.............AGG...STGLGGSL.SavID.....KLK.S.............A.....Q......SA....D.DE
 1276- 1348 (54.78/ 9.82)	LSA......L.TQ......SSSAT.SAPS...............LTAALTSGKASDGA.......F..........PsstKSP.................H.............YTS...STSGSSKA.P..FQ.....KSN.S.......fgSLGGSggtglK......AS......KV
 1526- 1600 (45.99/ 6.76)	SSDgsmfptA.GR......ASPGMlSGSA...............SRDGFRSG.GGSGS.......A..........P...GTP..........T......TtssmvlppfpspiSGG...ASNDGD...S..GT.....LMR.P.........P................SR....P...
 1601- 1675 (52.92/ 9.17)	SST......IsNH......SHSSQ.DGIG...............GG....SGNSGDAV.......A..........G...STPavasmasmmmM......N.............AMS...AAAGGGHT.QqlIA.....ALA.A........kSGVLP.....P......PP....P.NN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      94.57|      22|      22|    1363|    1384|       3
---------------------------------------------------------------------------
 1363- 1384 (33.18/ 8.70)	SS...AYSSKEKGGSTSGRSKSSSA
 1385- 1409 (29.63/ 6.77)	SSltgAMLTGATGGTGSGTGSSSSA
 1452- 1473 (31.76/ 7.93)	SS...ATGTASTGSSESGEAGSKSA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      73.98|      16|      16|     825|     840|       4
---------------------------------------------------------------------------
  787-  801 (22.32/ 7.57)	MISAKNEPPSP.ALGA
  825-  840 (26.81/10.93)	TISGSGLEIIPLATSA
  842-  856 (24.85/ 9.46)	TVAASGFPS.PSVTSA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.72|      24|      26|    1220|    1245|       5
---------------------------------------------------------------------------
 1220- 1245 (35.60/19.17)	GLKDSAKSGSGLlpSGGTSSLANCVS
 1249- 1272 (42.11/17.62)	GIAGAGKNKSVL..SSSNSSFNPCFS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.77|      14|      16|     322|     335|       7
---------------------------------------------------------------------------
  322-  335 (22.88/12.59)	VLEASSANKLQI.QP
  338-  352 (18.88/ 8.76)	VVNAGSGSAASVlQP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.04|      17|      20|     423|     441|       8
---------------------------------------------------------------------------
  423-  441 (23.90/22.23)	HASAgsVTDLA..KGLLVS.LP
  445-  464 (26.14/16.02)	HCYF..LTDNPslRGMMVSsIP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.90|      12|      16|     757|     768|      10
---------------------------------------------------------------------------
  757-  768 (21.93/ 9.76)	GLPLFGETVPAA
  775-  786 (20.97/ 8.97)	GSSLGGTTTTNA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.13|      15|      27|      76|      90|      14
---------------------------------------------------------------------------
   76-   90 (25.59/21.09)	QMELLMERLRTKA..KS
  104-  120 (20.55/15.34)	RMSLLEKRYALDAveKS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.08|      13|      27|     479|     491|      19
---------------------------------------------------------------------------
  479-  491 (23.15/15.49)	RQQAVFNQLLSSC
  504-  516 (21.93/14.28)	RRLLVFEVSALSC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     103.40|      32|     224|    1484|    1520|      20
---------------------------------------------------------------------------
 1484- 1520 (45.34/28.73)	QTPVTTAAPSvgSSPVRQLSSTSPSGEASarlLDGSL
 1711- 1742 (58.06/25.31)	KSPAPSPLPF..ASPHSNASSTHAAGAAG...LDDDL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.67|      16|     339|      52|      69|      21
---------------------------------------------------------------------------
   52-   67 (28.95/13.13)	GGPSAGGTGTAGKVLL
  392-  407 (28.72/ 6.31)	GGSGAGEQQQAGEGSS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32134 with Med1 domain of Kingdom Metazoa
Unable to open file!