<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP32086

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMASAPIASASEQPLTSTNPQLGPEPYFTYPTPTPDQLEEELPPYFENENVSLGLVLDRFARKGNHDLRVLLGEVLPQLSIRERPKPIIEYAKTTRQALLKYLAVLRWKTSVDIASTSTGSSVANTAGPASFPTPHSNNDPNDNSPDAFAGKGKSKTQADESVIKGKVTDAKRIAHFMEHQNQQHEVAIEHVKHVTKVVEGLRERNPDLLTALSLLATGTYNRLPASLVDPYLPKPVLTNSAILKVLRRLNRQIRYRLRCLDYLPPELIVEDIKDGRMYARGDGWRAELTVVGFEDTSRWWLTGVEWGWKAKEKGVDDPGGRVTKKFTGEERQGILDIANGEVLPPRELPENEMEVDTVITEDPQVKKSEVVEVVKKTVDAPLVRVYNLLQHLSLSYRLEVLFTQAIALSQGKWRGQLIPEMDREDKTLRLKYWLRERPMQVQRQDSTSIKRPQAPATLPASRQTMVGGIIHITLAEKTTPRSELDNLLSDIACGGLVPSERVLRLELNVKWVVDEAGVGGGLKSGDVMDNSMLHLDPDALSVEDLLHVCTRTHASHLTRSHGTSLLTSPRFLNASSVQPSLQESTDPSARPLTLRVPLPSRYAVSHLLIGVSSFSGLLEVEDEGAKGNDARAERAAMSMKSVNEGKTKLVDDITRLTTAIVVENIEAQMRQLGWKPTRQIALRLKDLIKADLHPVTTVFVPLASSPNHYFVTKAVQNGIVFELLKVAKVPNENGLGMKYMVGDRTMMDLGKLKARRKSGDDKLQRSAFEVQNKDLKDLFILSNALVAQTIVEQQLKDRSIPYIQQFPPTAGPASPKSTSPLAGMVPTICVDVRDLLRAGRGGGSAAMEVAMPKVWLQIEGWWEGGKCEVVTIVVLRHQPSTVAPGTVSQEGEDEKDRGESAKAEGISFDPKSSIVRFRAKDISRCVPAFLEQWERLSKVIVVAGEVSRLNKTESFKDVKILSFDLRTATLQYAPGHNASITYSPTDDSYRITFFLSSPSSDTDRNPHELVAPLLSARLNELTSTGIAGENKPKGQVGREFMGLLKNTLPVLKIGKILETKMGWGVVVLGVAKYRLIKDHNGKRFSVDVTLLPDLEHYLIQDGATPRGPDHVVDVYTGPLTSLEMDKVVHAVFDAEKSVSLAETGDEKGQGKKRHVPPVMKLDQGKSLVCEVARFERVLRKVMEEASKVMGLGNNMEMDS
Length1199
PositionTail
OrganismCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Agaricomycotina> Tremellomycetes> Tremellales> Cryptococcaceae> Cryptococcus> Cryptococcus neoformans species complex.
Aromaticity0.06
Grand average of hydropathy-0.336
Instability index36.43
Isoelectric point8.14
Molecular weight132641.41
Publications
PubMed=15653466

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP32086
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     165.20|      55|     267|     601|     662|       1
---------------------------------------------------------------------------
  601-  662 (82.13/65.67)	RYAVSHLLIGVSSFSGllevEDEGAKGNDARAERAAMSMK.SVNEGKTKlvdDIT...........RLTTAIVV
  876-  942 (83.08/50.83)	RHQPSTVAPGTVSQEG....EDEKDRGESAKAEGISFDPKsSIVRFRAK...DISrcvpafleqweRLSKVIVV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     139.43|      35|     272|      53|      89|       2
---------------------------------------------------------------------------
   55-   89 (62.23/47.91)	VLDRFARKGNHDLRVLLGEVLPQLSIRERPKPIIE
  245-  270 (39.46/21.08)	VLRRLNRQIRYRLRCL..DYLPPELIVE.......
  331-  354 (37.74/23.03)	......RQGILD..IANGEVLPP...RELPENEME
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     242.57|     116|     559|     425|     597|       3
---------------------------------------------------------------------------
  146-  238 (72.45/26.84)	DAFAGKG.KSKTQADESVIKGKvTDAKRIAHFMehqnqqHEVAIEHVKHVTkvveglRERNPDLLTALSLlatgtynrLPASLVDPYL.......PKPvLT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
  464-  514 (30.64/73.91)	...................................................................................................................................................................................................................................................................................................................................................................................nsailkvlrrlnrqiryrlrcldylppelivedikdgrmyargdgwraeltvvgfedtsrwwltgvewgwkakekgvddpggrvtkkftgeerqgildiangevlpprelpenemevdtvitedpqvkksevvevvkktvdaplvrvynllqhlslsyrlevlftqaialsqgkwrgqlipemdredktlrlkywlrerpmqvqrqdstsikrpqapatlpasrqTMVGGIIHITLAEKTTPRSElDNLLsDIACGGLVPservLRLELNVKWVVD
  515-  593 (92.37/63.29)	EAGVGGGlKSGDVMDNSMLHLD.PDALSVEDLL......HVCTRTHASHLT......RSHGTSLLTSPRF........LNASSVQPSLqestdpsARP.LT..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................
 1089- 1133 (47.11/ 9.47)	.....................................................................................................lrvplpsryavshlligvssfsgllevedegakgndaraeraamsmksvnegktklvdditrlttaivvenieaqmrqlgwkptrqialrlkdlikadlhpvttvfvplasspnhyfvtkavqngivfellkvakvpnenglgmkymvgdrtmmdlgklkarrksgddklqrsafevqnkdlkdlfilsnalvaqtiveqqlkdrsipyiqqfpptagpaspkstsplagmvpticvdvrdllragrgggsaamevampkvwlqiegwweggkcevvtivvlrhqpstvapgtvsqegedekdrgesakaegisfdpkssivrfrakdisrcvpafleqwerlskvivvagevsrlnktesfkdvkilsfdlrtatlqyapghnasitysptddsyritfflsspssdtdrnphelvapllsarlneltstgiagenkpkgqvgrefmgllkntlpvlkigkiletkmgwgvvvlgvakyrlikdhngkrfsvdvTLLPDLEHYLIQDGATPRGP.DHVV.DVYTGPLTS....LEMDKVVHAVFD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     113.76|      36|     559|     271|     321|       4
---------------------------------------------------------------------------
  271-  321 (44.49/69.36)	DIKDGRMYARGDGwRAELTVvgfeDTSRWWLTgVEwGWKakekgvddPGGR
  831-  866 (69.27/44.50)	DVRDLLRAGRGGG.SAAMEV....AMPKVWLQ.IE.GWW........EGGK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     136.12|      44|     618|     386|     431|       5
---------------------------------------------------------------------------
  386-  431 (69.29/45.18)	YNLLQHLsLSYRLEVLFTQAIAlSQGKWRGQLIPE.MDREDKTLR.LK
 1007- 1052 (66.83/36.02)	HELVAPL.LSARLNELTSTGIA.GENKPKGQVGREfMGLLKNTLPvLK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.21|      13|      15|      15|      27|       7
---------------------------------------------------------------------------
   15-   27 (27.63/18.98)	TSTNPQLGPE..PYF
   31-   45 (22.58/13.87)	TPTPDQLEEElpPYF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.20|      12|     682|     125|     145|       8
---------------------------------------------------------------------------
  125-  145 (18.24/26.01)	TAGPASfptphsnndPNDNSP
  809-  820 (24.97/11.20)	TAGPAS.........PKSTSP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP32086 with Med14 domain of Kingdom Fungi
Unable to open file!