<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31866

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPGRLIMDQQGAIGSQDLKKDRSQAQIASLPKKPDFAQHAPAPQANGLDTHAVNGTAPNGMPTHNAPPPTTNGSAAPTDTAMADTQPPPALDHSWQNDHNVWKSMGKMIERTSQACYSDLLDNISKLSETPLAVPESQPNGVVPQGIDTSEQSVNKKRTFMEFAKNQRDRFMKAAVLADWAKTTGDDMTTLIDVRYAQGQEQRAQDTAIWSIGNTKKATESIKQRSPNIQGALDLLATGKAAHLPNFNFVQPKRMTAKQLLKTLQGMNVTLATRLNLHEDLPAHFNNFTIANGRATFRVPGEFEVDLSVADEEPTSQFYMIDIRFLFNPAPSGLHENLRAVLEARTNTELSTKGLQGCYNFLHNFVLTHKINVLNNQVVKISREKWFDCLIVQSHRRVFTLQYWTALPGRKSWIEIGVSTGKDSKRRSRRPSNPQLSVRWFRRGEEMRDAKLNIDWETLNIEQILSEVIAKHISWSLTAIRDRLQMLAVPNSRLDAQLDTSESAPEDCSLRLSLSGLQDTTVVRLEPVTGKLFVSPANQRATRYESVINGDEKVDPATVLASGLCDMLKDRIDVAANLAAWIQLREDIPKDNLKQVFGNVAFRAYVCSKGWGKQWSLFTTFSLAGNKWWVVRFADRPNTDRPGPVRIIAETRAIPVDDAPSGQATVSRASLLRIEKAAAAEVSCAVLAQQLAAVNIRHKHDVLAPLFLHDASEAFQGHERETSLFLGDSDYLKDMFGKGSTITSLRVTHNGLIVKQSGDDDDLSMAKHDLRLTVKQGQLKHLQQYLASKSRDTDVTMNASGALALRLCTPFGAPYLDDICARLKACQRLNSYIASINACKFRPTIVNLSEVAFVYSEAPELTATIVFADDRGSLATLALEPKAYNPHFRVRIIFEKLLNMNVSKGFAALCHTLRISLPLLSALGKLESAHLFPKDMFVHVRHPTWYTINYKAPLPELTIMMELKDKQMSSGLLRAWSITTNLSKEDLAAHSAGKALLELSKAQNDSWHGLGDGSFVALLNGAAEAIERIDQTVRSAAAGTAIAAKQDEASDAQTTAQTPAQSKPATTSGPSGAKPATSAKGSVKGNVSAIGRPAAKPNVAKIKKEIIELD
Length1110
PositionTail
OrganismDothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight fungus) (Mycosphaerella pini)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Dothideomycetidae> Mycosphaerellales> Mycosphaerellaceae> Dothistroma.
Aromaticity0.06
Grand average of hydropathy-0.327
Instability index41.62
Isoelectric point8.93
Molecular weight122079.56
Publications
PubMed=23209441
PubMed=23236275

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31866
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.98|      14|      17|     656|     671|       1
---------------------------------------------------------------------------
  656-  671 (18.42/17.55)	VDDAPSgqATVSRASL
  674-  687 (22.56/13.16)	IEKAAA..AEVSCAVL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.77|      14|      17|     524|     537|       2
---------------------------------------------------------------------------
  524-  537 (25.33/16.27)	RLEPV.TGKLFVSPA
  543-  557 (21.44/12.68)	RYESViNGDEKVDPA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.14|      15|      16|     426|     440|       3
---------------------------------------------------------------------------
  426-  440 (27.94/17.15)	RRSRRPSNPQLSVRW
  442-  456 (27.19/16.49)	RRGEEMRDAKLNIDW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.08|      11|      16|     700|     710|       4
---------------------------------------------------------------------------
  700-  710 (20.96/13.03)	HDVLAPLFLHD
  718-  728 (20.12/12.22)	HERETSLFLGD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.03|      15|      16|     282|     296|       5
---------------------------------------------------------------------------
  282-  296 (27.18/14.89)	PAHFN.NFTIANGRAT
  300-  315 (21.85/10.71)	PGEFEvDLSVADEEPT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.15|      13|      16|     981|     993|       6
---------------------------------------------------------------------------
  981-  993 (21.69/14.81)	NLSK.EDLAAHSAG
  998- 1011 (20.46/13.49)	ELSKaQNDSWHGLG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.96|      16|      17|     831|     846|       8
---------------------------------------------------------------------------
  831-  846 (28.89/22.53)	SYIASI..NACKFRPTIV
  849-  866 (21.07/14.16)	SEVAFVysEAPELTATIV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      29.71|      10|      17|     749|     759|       9
---------------------------------------------------------------------------
  749-  759 (14.89/13.08)	HN.GLIVKQsGD
  768-  778 (14.83/ 7.34)	HDlRLTVKQ.GQ
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31866 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LSETPLAVPESQPNGVVPQGIDTSEQSVNKKRTFME
2) MPGRLIMDQQGAIGSQDLKKDRSQAQIASLPKKPDFAQHAPAPQANGLDTHAVNGTAPNGMPTHNAPPPTTNGSAAPTDTAMADTQPPPALDHSWQNDHNVWKSMGKM
3) TAIAAKQDEASDAQTTAQTPAQSKPATTSGPSGAKPATSAKGSVKGNVSAIGRPAA
127
1
1040
162
108
1095










Molecular Recognition Features






MoRF SequenceStartStop




1) NVSAIGRPAAKPNVAKIKKEIIELD
1086
1110