<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31831

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQREEKQLEASLDALLNQVADLKNSLGSFIYKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPGDTNALVAAVAFGKGLSNWRPSGSSGPGQPGQPGAGTILAGASGLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
Length268
PositionHead
OrganismMus musculus (Mouse)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Muridae> Murinae> Mus> Mus.
Aromaticity0.04
Grand average of hydropathy-0.601
Instability index44.30
Isoelectric point6.45
Molecular weight29198.70
Publications
PubMed=16141072
PubMed=15489334
PubMed=21183079

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). 
ECO:0000250
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IDA:MGI
nucleoplasm	GO:0005654	TAS:Reactome
GO - Biological Function
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IBA:GO_Central
transcription coregulator activity	GO:0003712	IBA:GO_Central
GO - Biological Process
protein ubiquitination	GO:0016567	IEA:UniProtKB-UniPathway
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central

Interaction

Binary Interactions
[Q9D7W5<-->Q9R0X0: Med20]	NbExp=2	EBI-7990252,EBI-398698
[Q9D7W5<-->Q9CQI9: Med30]	NbExp=2	EBI-7990252,EBI-309220
[Q9D7W5<-->Q9NVC6: MED17]	Xeno	NbExp=2,
[Q9D7W5<-->Q15528: MED22]	Xeno	NbExp=2,
[Q9D7W5<-->Q6P2C8: MED27]	Xeno	NbExp=2,
[Q9D7W5<-->Q9H204: MED28]	Xeno	NbExp=2,
[Q9D7W5<-->Q9NX70: MED29]	Xeno	NbExp=2,
[Q9D7W5<-->O75586: MED6]	Xeno	NbExp=2,

Repeat regions

Repeats

>MDP31831
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.15|      23|      27|     165|     191|       1
---------------------------------------------------------------------------
  165-  191 (35.13/25.35)	GLRPNKQTFNPGDTNALVAAvafgKGL
  198-  220 (42.03/21.25)	GSSGPGQPGQPGAGTILAGA....SGL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.18|      25|      28|      67|      92|       2
---------------------------------------------------------------------------
   67-   92 (40.28/34.38)	KTPLFRNQViIP..LVLSPDRD.EDLMRQ
   96-  123 (35.90/25.06)	RVPVFSHEV.VPdhLRTKPDPEvEEQEKQ
---------------------------------------------------------------------------


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31831 with Med8 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) CSNLLEKISKEERESESGGLRPNKQTFNPGDTNALV
2) FSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQ
3) LSNWRPSGSSGPGQPGQPGAGTILAGASGLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
147
100
191
182
137
268










Molecular Recognition Features






MoRF SequenceStartStop




1) GIKTNIKSASMHPYQR
253
268