<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31829

Description Isoform 3 of Mediator of RNA polymerase II transcription subunit 8
SequenceMQREEKQLEASLDALLSQVADLKNSLGSFICKLENEYGRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
Length179
PositionHead
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.04
Grand average of hydropathy-0.547
Instability index44.70
Isoelectric point6.92
Molecular weight29079.60
Publications
PubMed=12149480
PubMed=16710414
PubMed=15489334
PubMed=10235267
PubMed=12584197
PubMed=15175163
PubMed=15989967
PubMed=19369195
PubMed=21269460
PubMed=24275569

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. 
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IDA:MGI
nucleoplasm	GO:0005654	TAS:Reactome

GO - Biological Function
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IBA:GO_Central
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
protein ubiquitination	GO:0016567	IEA:UniProtKB-UniPathway
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome









Interaction





Binary Interactions
[Q96G25<-->Q15370: ELOB]	NbExp=5	EBI-394405,EBI-301238
[Q96G25<-->Q15369: ELOC]	NbExp=3	EBI-394405,EBI-301231
[Q96G25-2<-->Q6A162: KRT40]	NbExp=3	EBI-10286267,EBI-10171697
[Q96G25-2<-->Q8ND90: PNMA1]	NbExp=3	EBI-10286267,EBI-302345
[Q96G25-2<-->P31321: PRKAR1B]	NbExp=3	EBI-10286267,EBI-2805516












Repeat regions





Repeats

>MDP31829
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.18|      25|      28|      67|      92|       1
---------------------------------------------------------------------------
   67-   92 (40.28/31.81)	KTPLFRNQViIP..LVLSPDRD.EDLMRQ
   96-  123 (35.90/23.21)	RVPVFSHEV.VPdhLRTKPDPEvEEQEKQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      58.11|      17|      37|     192|     209|       2
---------------------------------------------------------------------------
  192-  209 (31.60/21.97)	SNWRP..SGSSgPGQAGQPG
  230-  248 (26.51/12.83)	SQQQPmlSGVQ.MAQAGQPG
---------------------------------------------------------------------------











Associated diseases
Disease
renal cell carcinoma	PMID:27050271



























Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31829 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADA
2) LEKISKEERESESGGLRPNKQTFNPTDTNALV
3) LSNWRPSGSSGPGQAGQPGAGTILAGTSGLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
100
151
191
135
182
268










Molecular Recognition Features






MoRF SequenceStartStop




1) IKTNIKSASMHPYQR
254
268