<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31825

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQQREEKQLEAFLESLVARVAHLKGSLQSFIYKLENEYDRLTWPSVLDNFALISGQLNTINKLLRNEKTPSYKSQVIIPLLLSPDRDEELAKLTEHRVPVFSHEIVPDHLRTKPDPEVEEQEKHLSAEAARIGPEVAQKQIQALNKLCSNLLEKLNNPREDRDSETSALRQNKPSFNPADTNALVAAVGFGKGLSKCRPPGPVAPGHPGQPMMQTGPTLQQVTIAGASGHQAGMSGPVAPQQPGQPGKIPSNIKTNIKSASMHPYNR
Length267
PositionHead
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Actinopterygii> Neopterygii> Teleostei> Ostariophysi> Cypriniformes> Danionidae> Danioninae> Danio.
Aromaticity0.04
Grand average of hydropathy-0.609
Instability index43.57
Isoelectric point8.46
Molecular weight29402.08
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. May play a role as a target
recruitment subunit in E3 ubiquitin-protein ligase complexes and thus
in ubiquitination and subsequent proteasomal degradation of target
proteins (By similarity). 


ECO:0000250	

GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IBA:GO_Central
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
protein ubiquitination	GO:0016567	IEA:UniProtKB-UniPathway
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31825
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     106.11|      28|      32|     201|     230|       1
---------------------------------------------------------------------------
  201-  230 (51.07/26.18)	GPVAPGHPGQPmmQTGPTLQQVTIAGASGH
  236-  263 (55.03/23.42)	GPVAPQQPGQP..GKIPSNIKTNIKSASMH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      72.45|      24|      27|      62|      87|       2
---------------------------------------------------------------------------
   62-   87 (33.34/30.19)	KLlRNEKTPSYkSQVIIP..LLLSPDRD
   92-  117 (39.12/25.19)	KL.TEHRVPVF.SHEIVPdhLRTKPDPE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31825 with Med8 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LSKCRPPGPVAPGHPGQPMMQTGPTLQQVTIAGASGHQAGMSGPVAPQQPGQPGKIPSNIKTNIKSASMHPYNR
2) NLLEKLNNPREDRDSETSALRQNKPSFNPADTNALV
194
150
267
185










Molecular Recognition Features






MoRF SequenceStartStop




1) KSASMHPY
258
265