<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31726

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNEISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEPTETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF
Length239
PositionMiddle
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Taphrinomycotina> Schizosaccharomycetes> Schizosaccharomycetales> Schizosaccharomycetaceae> Schizosaccharomyces.
Aromaticity0.08
Grand average of hydropathy-0.820
Instability index62.88
Isoelectric point4.80
Molecular weight27340.17
Publications
PubMed=11859360
PubMed=10625684
PubMed=11572939
PubMed=18257517

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
cytosol	GO:0005829	HDA:PomBase
mediator complex	GO:0016592	IDA:PomBase
nucleus	GO:0005634	HDA:PomBase
F:transcription coactivator activity	GO:0003713	IPomBase
P:positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IPomBase

GO - Biological Function
transcription coactivator activity	GO:0003713	IC:PomBase
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IC:PomBase
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31726
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.43|      15|      51|     117|     131|       2
---------------------------------------------------------------------------
  117-  131 (31.60/18.37)	FSSAPPGYN.P...ETGQD
  170-  188 (20.83/ 9.96)	FSEQPPKTNePtetETEID
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31726 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) SATASQLFSEQPPKTNEPTETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDL
163
230










Molecular Recognition Features






MoRF SequenceStartStop




1) ANKDVFAGFDLFDPEMEEDF
2) KTKMNY
220
196
239
201