<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31659

Description Mediator of RNA polymerase II transcription subunit 2
SequenceMVPKTGVSDRQENKLTQYFDDILRLSADLLTQQQLKTIKLDPKVTTGFSKAQQKGLGDRITQFYSLLDTLDVSLQTTADYVGAVKDSALQLKKQREEEQIKKQEQEKLERQLLEQQKLEQQQLEQKQLKQKQQQQQQQQQQQQQQQPQEQQHEQQQRYSAKNTPIDMLTNFDTDLPSAGITQAQSFNSEFGDLNGMDLSMFDSMDNQVGFGGLQNTSGGNEKKNDPQINFNDTNAPPSAVNVPENGNPSSYLTLNDFNDLGIDWNAANDNNELNLEDFNI
Length280
PositionTail
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kluyveromyces.
Aromaticity0.06
Grand average of hydropathy-1.084
Instability index49.45
Isoelectric point4.52
Molecular weight31889.59
Publications
PubMed=15229592

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 
ECO:0000250
GO - Cellular Component
nucleus	GO:0005634	IEA:UniProtKB-SubCell
GO - Biological Function
GO - Biological Process








Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31659
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.78|      15|      16|     125|     140|       1
---------------------------------------------------------------------------
  125-  139 (27.16/ 9.88)	QKQLKQKQQQQQQQQ
  143-  157 (27.62/ 6.51)	QQQQPQEQQHEQQQR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.82|      17|      19|     242|     258|       2
---------------------------------------------------------------------------
  242-  258 (32.62/22.04)	VPEN.GNPSSYLTLNDFN
  262-  279 (27.20/17.15)	IDWNaANDNNELNLEDFN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.63|      15|      17|     180|     194|       3
---------------------------------------------------------------------------
  180-  194 (25.73/15.22)	ITQAQSFNSE..FGDLN
  198-  214 (21.90/11.87)	LSMFDSMDNQvgFGGLQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.31|      15|      16|      85|      99|       4
---------------------------------------------------------------------------
   85-   99 (23.47/11.62)	KDSALQLKKQREEEQ
  102-  116 (23.84/11.90)	KQEQEKLERQLLEQQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      35.67|      10|      15|      40|      49|       5
---------------------------------------------------------------------------
   40-   49 (17.62/11.47)	LDPKVTTGFS
   56-   65 (18.04/11.89)	LGDRITQFYS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31659 with Med2 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LEQKQLKQKQQQQQQQQQQQQQQQPQEQQHEQQQRYSAKNTPIDMLTNFDTDLPSAGITQAQSFNSEFGDLNGMDLSMFDSMDNQVGFGGLQNTSGGNEKKNDPQINFNDTNAPPSAVNVPENGNPSSYLT
123
253










Molecular Recognition Features






MoRF SequenceStartStop




1) LNLEDFNI
2) LTLNDFNDLGIDWNAAND
3) YFDDILRL
273
252
18
280
269
25