<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31618

Description Mediator of RNA polymerase II transcription subunit 26
SequenceMNQIEIQELTTHLSQALDQNYDVVNMDAVLCVICALEGTTITKEQLEATRLAKYINQLRRRTKNEHLARRAKSLLKKWREMVGIQQTATENLAHPSQISSSQPALDLVKSPITSFITEPIAPSQQIVSDLHSNIDSAEPPLSGEHTLLHPNFSNLVNSIKDSDRHENIAITTLHTHKDRRHSHSIRSCHVASPQPIVIDHSVNSVINLTDDSTVKINEASVVIDIASDSDENDNNGSLNPKKLNSIVPAPLPIPSTPSSRQRKLKKEKRSKEREGQVATNTRFGAKASDGFQQAALTTDSEIFSLSNSSMSSIVSGDATSSYSQNKSRLNSNELTFTGRFKAVDHLFRSTESGKHKIDEYSAYDSNASCSRLSPSTVDEVKKAEHLFDAQLTNTTANQIPMSAVSIGYESNTRHEYLESDSPSQIPKRRGRKKGSKGVDALIAKESSSLSQQIFFGGSTVKKVKTTKELFNEIQSRKLSVSMQSSASNLSNSSTNRDLPSHTTFPRQTSSCSDTSMNSPHILETLSGSAIFASKIDDLGNTDSDTVTSDPSHDSNKSQEIKECTSLDSNSNSIQSLPLANNRENLIPTNVNDITTQLMHLIHSLNSPLSEIEIERVYQEKIIPCTCIVIEGVQGTLGESNNSTLDEQSYNAKISLNSKDNLSCHNQDNYSTSQRSVNGEVIDTQPKLVKSIFDLDFDDNDDPLYFIMDEIQKPIARADELKNNKSDTKNINFGASAQNASLNIIHFNDDAQQDNSNQERGKNETQNAIPAFTVHEDPDCLARQRFYIQTNKVTSFHINALHNYYIPNINGNWDSVESFTSFQSEHTIMDTMESYTVTNGADVVPKYGLLTSDRIRKDLSSLKSIKPYRVKNFTSLISPFLGVAKCLPTCRRARRRFKNFVNSSTANDKISNNFESLENMKTSFKNCNPLNVKIDGTTSAIYSHNENHVPMQVNTNVEQLPPSQSFSSNAISYNLLKLADDKVCHDGISDKSSNTGCQGNSPYSSSSSSSYSGRKEDQHIITKNLQNKNIQLNSRKSDMKRRKRIYLENDSKKKKHYRKRIKTAVNRTFSNLHRNLYSIDSENGMKNDYKNEYGNSNNEEYAIVQRPAGDGENCSNHIVLTIKKTPSKINSPANSMTAFSPPSTSDGANLQKSILDLRLEDTNISQSITEIPQKKDLSKTDRIVHQSRSSAYRRIFRKPDKSQTKFIDLDLKHLFHLKANSPCISANAKLHNKLFFPHELSRDNTSGIRERLINYSSSSSSSYDDDSEVENTSFLKKANVNKFSASNNRNLKTETETCAATAISEFKFESDEDSILTSLGDSDIDIQDEIQVINDKVDYCNDFQNNKMLESFNSIFVDSLRTNTKNASDNMGSSAHITSLESLKPENADAITRYCNNNNLGVNLSNELSNISAKPLKSFDSTMKLPSLGIEHCTGIKTSSDMASTNIAEKKLTRIQQFKEWHQVLQLRSYNNEPLIVLPYVLLE
Length1483
PositionUnknown
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.06
Grand average of hydropathy-0.704
Instability index47.52
Isoelectric point6.77
Molecular weight165513.86
Publications
PubMed=10731132
PubMed=12537572
PubMed=12537569
PubMed=16751183
PubMed=18327897

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for activated transcription of the MtnA gene. 
ECO:0000250	
ECO:0000269	PubMed:16751183
GO - Cellular Component
chromatin	GO:0000785	IDA:FlyBase
core mediator complex	GO:0070847	IPI:FlyBase
mediator complex	GO:0016592	ISS:UniProtKB
nucleolus	GO:0005730	IDA:FlyBase
pericentric heterochromatin	GO:0005721	IPI:FlyBase
polytene chromosome chromocenter	GO:0005701	IDA:FlyBase
GO - Biological Function
transcription coregulator activity	GO:0003712	ISS:UniProtKB
GO - Biological Process
positive regulation of gene expression	GO:0010628	IMFlyBase
regulation of transcription by RNA polymerase II	GO:0006357	IMUniProtKB

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31618
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      71.65|      23|      24|      89|     112|       1
---------------------------------------------------------------------------
   89-  112 (35.01/29.57)	TENLAhPSQ..ISSSQPALDLVKSPI
  117-  141 (36.64/24.63)	TEPIA.PSQqiVSDLHSNIDSAEPPL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     277.69|      68|     249|     962|    1029|       2
---------------------------------------------------------------------------
  962- 1029 (115.49/64.56)	SQSFSSNAISYNLLKLADDKV........................CHDGIS.DK...........SSNTGCQGNSPYSSSS...SSSYSGRKEDQHIITKNLQNKNI
 1220- 1279 (82.73/43.79)	.....SPCISAN.AKLHNKLF........................FPHELSrDN...........TSGIR.ERLINYSSSS...SSSYDDDSEVEN..TSFLKKANV
 1294- 1399 (79.47/41.72)	TETCAATAISEFKFESDEDSIltslgdsdidiqdeiqvindkvdyCNDFQN.NKmlesfnsifvdSLRTNTKNASDNMGSSahiTSLESLKPENADAITRYCNNNNL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     100.87|      33|     163|     893|     927|       4
---------------------------------------------------------------------------
  893-  927 (55.77/40.07)	RRRFKNFVNSSTANdkISNNFESL..EN.MKTSFK....NCN
 1057- 1096 (45.10/25.66)	RKRIKTAVNRTFSN..LHRNLYSIdsENgMKNDYKneygNSN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     236.84|      77|     330|     378|     455|       5
---------------------------------------------------------------------------
  254-  301 (32.57/10.60)	...................................PS.TPS.SRQRKlkkeKRSKEREGQVATNTR.......FgakasDGFQQAALTTDSE
  305-  390 (99.60/46.65)	LSNSSMSSIVSGDATSSYSQNkSRlnsNELTFTGRFKAVDHlFRSTE....SGKHKIDEYSAYDSNASCSRL..spstvDEVKKAEHLFDAQ
  391-  471 (104.68/53.02)	LTNTTANQIPMSAVSIGYESN.TR...HEYLESDSPSQIPK.RRGRK....KGSKGVDALIAKESSSLSQQIfFggstvKKVKTTKELFN..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.28|      22|      26|     722|     746|       6
---------------------------------------------------------------------------
  722-  746 (33.40/23.26)	NNKSDTKNINFGAS.AQNAslnIIHF
  749-  771 (34.87/17.09)	DAQQDNSNQERGKNeTQNA...IPAF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.89|      16|      26|    1164|    1179|       7
---------------------------------------------------------------------------
 1164- 1179 (26.69/15.01)	SQSITEIPQKKDLSKT
 1188- 1203 (28.20/16.28)	SSAYRRIFRKPDKSQT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      32.38|      11|      26|     191|     204|       8
---------------------------------------------------------------------------
  191-  204 (14.81/18.60)	ASpqpIVIDHSVNS
  219-  229 (17.57/ 9.16)	AS...VVIDIASDS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.23|      17|     158|     510|     542|      10
---------------------------------------------------------------------------
  510-  526 (30.45/40.88)	SCSDTSMNSPHILETLS
  549-  565 (30.79/ 8.27)	DPSHDSNKSQEIKECTS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.49|      24|      27|     642|     667|      11
---------------------------------------------------------------------------
  642-  667 (33.21/29.14)	STlDEQSYNAKIsLNSKDNL..SCHNQD
  670-  695 (35.28/20.94)	ST.SQRSVNGEV.IDTQPKLvkSIFDLD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.33|      16|      33|    1400|    1415|      12
---------------------------------------------------------------------------
 1400- 1415 (26.63/17.34)	GVNLSNELS..NISAKPL
 1434- 1451 (21.70/12.51)	GIKTSSDMAstNIAEKKL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      68.82|      24|      28|     794|     820|      13
---------------------------------------------------------------------------
  794-  820 (34.87/32.97)	SFH..INALHNYYIpnINGNwDSVESF...TS
  823-  851 (33.95/18.84)	SEHtiMDTMESYTV..TNGA.DVVPKYgllTS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.81|      12|      33|    1106|    1117|      15
---------------------------------------------------------------------------
 1106- 1117 (24.32/12.80)	PAG..DGENCSNHI
 1140- 1153 (17.48/ 7.19)	PPStsDGANLQKSI
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31618 with Med26 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DRHENIAITTLHTHKDRRHSHSIRSCHVASP
2) SDKSSNTGCQGNSPYSSSSSSSYSGRKEDQHIITKNLQNKNIQLNSRKSDMKRRKRIYLENDSKKKKHYRKRIKTAV
3) SNTRHEYLESDSPSQIPKRRGRKKGSKGVDAL
4) SVVIDIASDSDENDNNGSLNPKKLNSIVPAPLPIPSTPSSRQRKLKKEKRSKEREGQVATNTRFG
5) VSMQSSASNLSNSSTNRDLPSHTTFPRQTSSCSDTSMNS
163
988
410
220
480
193
1064
441
284
518










Molecular Recognition Features






MoRF SequenceStartStop




1) AYRRIFRK
1190
1197