<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31613

Description Isoform 4 of Mediator of RNA polymerase II transcription subunit 25
SequenceMVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI
Length102
PositionUnknown
OrganismMus musculus (Mouse)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Muridae> Murinae> Mus> Mus.
Aromaticity0.05
Grand average of hydropathy-0.197
Instability index58.75
Isoelectric point8.60
Molecular weight78111.79
Publications
PubMed=16141072
PubMed=15489334
PubMed=17641689
PubMed=24129315

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity). 
ECO:0000250
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central
nucleoplasm	GO:0005654	ISO:MGI
transcription regulator complex	GO:0005667	IPI:MGI
GO - Biological Function
retinoic acid receptor binding	GO:0042974	IPI:UniProtKB
retinoid X receptor binding	GO:0046965	IPI:UniProtKB
transcription coactivator binding	GO:0001223	ISO:MGI
transcription factor binding	GO:0008134	ISO:MGI
GO - Biological Process
negative regulation of fibroblast proliferation	GO:0048147	ISO:MGI
negative regulation of transcription by RNA polymerase II	GO:0000122	ISO:MGI
positive regulation of cell cycle arrest	GO:0071158	ISO:MGI
positive regulation of chromatin binding	GO:0035563	ISO:MGI
positive regulation of mediator complex assembly	GO:2001178	ISO:MGI
positive regulation of transcription by RNA polymerase II	GO:0045944	IGI:MGI

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31613
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     510.92|      68|      68|     565|     632|       1
---------------------------------------------------------------------------
  193-  270 (57.07/10.71)	.....LLFEKAAPPALLEPLQQ....PADVSQDprhmvlvrglvlPVGGSSTSGSLQTKQAVP..LPP.APA..SaatlsaaP.PQALPPVPP...
  271-  319 (55.00/10.05)	.........QYQVPGNL...SA....AQVAAQN............AV..EAAKSQKAGLG......PRFSPI..N.......PlQQAAPGVGP..P
  326-  382 (69.50/14.65)	PPLAPVPPG............A....PKPPPAS............Q.PSLVSTVAPGPVLAAP..AQPGAPSlaG.....tvT.PGGVNG..PSAA
  459-  520 (72.71/15.66)	TTLGPLFRN.SR....MVQFHF....TNKDLES............LKGLYRIMGNGFA.GCVH..FPHTAPC..E.......V.RVLMLLYSSKKK
  565-  631 (121.48/31.12)	PVLGPILEEQARPPQNLLQLRA....PQPQPQG............AVGASAATGQPQPQGATQ..APTGAPQ..G.......P.PGAAPGPPPSG.
  632-  691 (71.02/15.13)	....PILRPQ.NPGAN.PQLRSlllnPAP.PQ....................TGVPPPQASLHhlQPPGAPT.lL.......P.PHQSMGQPQLGP
  692-  734 (64.15/12.95)	QLLHPP.PAQSWPTQ..LPQRA....PLP........................GQM.........LLSGGPR..G.......PvP..QPGLQPS..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      97.92|      30|     111|      39|      69|       2
---------------------------------------------------------------------------
   39-   69 (53.43/33.23)	YLLPAIEYFN.GGPPAETdFGGDYG..GTQYSLV
  151-  183 (44.49/22.88)	YLLPAVESTTySGCTTES.LVQKIGerGIHFSIV
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31613 with Med25 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSA
2) HKQVQQQKLEQQRGMGAQQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMD
3) VAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALG
237
544
283
280
743
390










Molecular Recognition Features






MoRF SequenceStartStop




NANANA