<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31612

Description Isoform 6 of Mediator of RNA polymerase II transcription subunit 25
SequenceMVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI
Length534
PositionUnknown
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.05
Grand average of hydropathy-0.249
Instability index60.51
Isoelectric point8.61
Molecular weight78169.79
Publications
PubMed=12163014
PubMed=14657022
PubMed=14983011
PubMed=11230166
PubMed=14702039
PubMed=15057824
PubMed=15489334
PubMed=15175163
PubMed=15989967
PubMed=17641689
PubMed=22814378
PubMed=20974256
PubMed=19290556
PubMed=25792360
PubMed=25527630

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription. 
ECO:0000269	PubMed:14657022
ECO:0000269	PubMed:14983011
ECO:0000269	PubMed:17641689
GO - Cellular Component
mediator complex	GO:0016592	IBA:GO_Central
nucleoplasm	GO:0005654	IDA:HPA
transcription regulator complex	GO:0005667	IBA:GO_Central
GO - Biological Function
retinoic acid receptor binding	GO:0042974	IPI:UniProtKB
retinoid X receptor binding	GO:0046965	IPI:UniProtKB
transcription coactivator binding	GO:0001223	IPI:UniProtKB
transcription factor binding	GO:0008134	IPI:UniProtKB
GO - Biological Process
negative regulation of fibroblast proliferation	GO:0048147	IMUniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	IMUniProtKB
positive regulation of cell cycle arrest	GO:0071158	IMUniProtKB
positive regulation of chromatin binding	GO:0035563	IMUniProtKB
positive regulation of mediator complex assembly	GO:2001178	IMUniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:UniProtKB
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome

Interaction

Binary Interactions
[Q71SY5<-->A8MQ03: CYSRT1]	NbExp=3	EBI-394558,EBI-3867333
[Q71SY5<-->Q6NXG1: ESRP1]	NbExp=3	EBI-394558,EBI-10213520
[Q71SY5<-->O00167-2: EYA2]	NbExp=3	EBI-394558,EBI-12807776
[Q71SY5<-->A1KXE4-2: FAM168B]	NbExp=3	EBI-394558,EBI-12193763
[Q71SY5<-->O14964: HGS]	NbExp=3	EBI-394558,EBI-740220
[Q71SY5<-->Q53G59: KLHL12]	NbExp=3	EBI-394558,EBI-740929
[Q71SY5<-->Q3LI64: KRTAP6-1]	NbExp=3	EBI-394558,EBI-12111050
[Q71SY5<-->Q14847-2: LASP1]	NbExp=3	EBI-394558,EBI-9088686
[Q71SY5<-->O60244: MED14]	NbExp=2	EBI-394558,EBI-394489
[Q71SY5<-->Q96RN5: MED15]	NbExp=2	EBI-394558,EBI-394506
[Q71SY5<-->Q9Y2X0: MED16]	NbExp=3	EBI-394558,EBI-394541
[Q71SY5<-->Q9ULK4: MED23]	NbExp=4	EBI-394558,EBI-311161
[Q71SY5<-->O75448: MED24]	NbExp=2	EBI-394558,EBI-394523
[Q71SY5<-->Q9NZQ3-3: NCKIPSD]	NbExp=3	EBI-394558,EBI-10963850
[Q71SY5<-->A1E959: ODAM]	NbExp=3	EBI-394558,EBI-5774125
[Q71SY5<-->Q8TDS5: OXER1]	NbExp=3	EBI-394558,EBI-12813389
[Q71SY5<-->Q9UBV8: PEF1]	NbExp=3	EBI-394558,EBI-724639
[Q71SY5<-->Q96CS7: PLEKHB2]	NbExp=3	EBI-394558,EBI-373552
[Q71SY5<-->P10276: RARA]	NbExp=10	EBI-394558,EBI-413374
[Q71SY5<-->Q92753-1: RORB]	NbExp=3	EBI-394558,EBI-18560266
[Q71SY5<-->Q9BVN2: RUSC1]	NbExp=3	EBI-394558,EBI-6257312
[Q71SY5<-->P19793: RXRA]	NbExp=4	EBI-394558,EBI-78598
[Q71SY5<-->Q9UJW9: SERTAD3]	NbExp=3	EBI-394558,EBI-748621
[Q71SY5<-->P51692: STAT5B]	NbExp=3	EBI-394558,EBI-1186119
[Q71SY5<-->Q92734: TFG]	NbExp=3	EBI-394558,EBI-357061
[Q71SY5<-->Q08117-2: TLE5]	NbExp=3	EBI-394558,EBI-11741437
[Q71SY5<-->Q70EL1-9: USP54]	NbExp=3	EBI-394558,EBI-11975223
[Q71SY5<-->Q9NZC7-5: WWOX]	NbExp=3	EBI-394558,EBI-12040603
[Q71SY5<-->O00308: WWP2]	NbExp=3	EBI-394558,EBI-743923
[Q71SY5<-->P28700: Rxra]	Xeno	NbExp=3,
[Q71SY5<-->G8HBG2: UL48]	Xeno	NbExp=5,

Repeat regions

Repeats

>MDP31612
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     518.68|      60|      60|     227|     286|       3
---------------------------------------------------------------------------
  227-  286 (108.05/26.51)	VLPVGGG.................S..AP...GPLQSK....QPV.PLPPAAP.S.G.A.............TLSAA...........PQQPLPPVPPQYQVPGNLSAAQVAAQ
  289-  343 (69.71/14.21)	VEAAKNQ.................K..AG..lGP..RF....SPItPLQQAAP.GvG.P.............PFSQA...........PAPQLPPGPPG..APKPPPAS....Q
  347-  392 (62.06/11.76)	VSTVAPG.................S..GL...AP..TA....Q......PGAP.S.M.A.............G.TVA...........PGGVSGPSPAQ......LGAPALGGQ
  405-  476 (54.01/ 9.18)	VLEWQEK.................P..KP...ASVDANtkltRSL.PCQVYVN.H.G.E.............NLKTEqwpqklimqliPQQLLTTLGPLFR...NSRMVQFHFT
  487-  550 (61.42/11.55)	YRIMGNG.................F..A....G..........CV.HFPHTAP.C.E.VrvlmllysskkkiFMGLI...........PYDQSGFVNGIRQVITNHK..QVQQQ
  559-  604 (59.27/10.86)	...MGGQ.................Q..APpglGPILED....Q.......ARP.S.Q.N.............LLQLR...........PPQP....QPQ....GTVGASGATGQ
  605-  670 (58.85/10.73)	PQPQGTAqpppgapqgppgaasgpP..PP...GPILRP....QN....PGANP.Q.L.R.............SLLLN...........PPPPQTGVPPPQASLHHL........
  689-  734 (45.32/ 6.39)	..PQLGP.................PllHP...PPAQS.....WPA.QLPPRAPlP.GqM.............LLSGG...........PRGPVP..QPGLQ.............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     108.82|      31|     111|      39|      69|       4
---------------------------------------------------------------------------
   39-   69 (58.34/33.84)	YLLPAIEYFNGGPPAETDFGGDYG..GTQYSLV
  151-  183 (50.47/28.25)	YLLPAVESTTYSGCTTENLVQQIGerGIHFSIV
---------------------------------------------------------------------------

Associated diseases

Disease
Charcot-Marie-Tooth (CMT) disease	PMID:19290556


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31612 with Med25 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALG
2) HKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMD
233
544
390
745










Molecular Recognition Features






MoRF SequenceStartStop




1) LMDLI
743
747