<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31596

Description Mediator of RNA polymerase II transcription subunit 23
SequenceMETQVIDTVNEFLKVDSLDEAFVSVIVFKPNTEQERATRFANDLVTAFGNVAQENREQVLRLYLLRAAGASGYHIKVLMAALVKLVDAHVITARMLCDKVLMCEKLDFEHRTFWIESFRLIKRVIVQVDYKGVREIMKVCRDKAQWFPLNVNVTYMPQLLAVEDILRFIFDRNNCLLPAYFIANEIMRPFPYHWKLNRLMTDFVEEFRTTAQMVSIIGHASMLPIVEHFGYADHMMNSWRLDHNTLKFNFKGSLPYEPELLEEQKPLLRYVLEQPYSREMVSQMLNLQKHQKQRYNALEEQLVNLIVQAMEMTEANDATAGSGFNSSDEQITPYEWMWLHLSSQLIYFVLFQFVSFMHIVLALHEKLSKLELRKGRDQLMWILLQFISGSIQKNPITNFLPVFRLFDLLYPELEPLKLPDINKSSMVRHMAPICVWIHLMKKARVENMNITRPLPIALKNHYDFLQHLVTANTMMNMTLGNDFRIILICNAYSTNQEYFGRPMGLLLDALNGTSKSPNGGQIPAVTFSVTVLDSLTVHSKMSLIHSFVTQMLKQAQSKGQVPAAALLETYARLLVYTEIESLGIKGFLSQLMPTVFKNHAWAMLHTLMEMFSYRLHHVPTHYRVQLLSLLHSLSSVPQTNKMQLNLCFESTALRLITSIGSAEFQPQFSRYFNDKSPGAVASNESEELNRVLILTLARSMHVHGGGDEMQGWCKDFLSNIIQHTPHSWPMHSLACFPPALNEYFTQNNQPPENKQQLKKAVEEEYRTWTSMTNENDIIAHFLRPTTNPLFLCLLFKIIWETENISPVAYKILEGISARALSTHLRKFCDYLVAEVASSSDGRDFIHKCVDTINNMIWKFNVVTIDRVVLCLALRTHEGNEAQVCFLIIQLLLLKASELRNRVQEFCKDNNPDHWKQSNWQDKHLSFHQKYPEKFALDESASQIPLPVYFSNVCLRFLPVLDVVVHRFIELTITNVHQILGFILDHLSILYKFHDRPITYLYNTLHYYERILRDRPALKKKLVGAITSAFSEIRPPNWSVSEPYKVYLQSQDSLWTPELSYYMSLIRRLADTISGKNVFYSTDWRFNEFPNAPTHALYVTCVELLGLPVAPPLVASNLIDVIVSGYAVIPQKDIHSYINAVGIVLAALPEPYWSGIYDRLQDMLNTPNMLNWTYRFNAFELFNFKTVREAMLEKTYAVVLAVAHSVFHHMGAFKLAAMTRYLKEKLKPCVRTEQQLLYLCHVFGPFLQRIELEKPNAVAGIAVLLYEILEIVDKHHGPKPLQYMDQICDFLYHIKYIHVGNIIKNESEAIIKRLRPLLQMRLRFITHLNLEDIHTEKINDNTSNNAITSQTQSPMQTQHQQQPQQPHQQQQQQQQQQQQQQQQQQQQQQMQQQQINAVQTTSVPLGSGGNLQQQQQINQQQQMYMQHMQQHQHMQNMRHN
Length1439
PositionTail
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.10
Grand average of hydropathy-0.179
Instability index43.26
Isoelectric point7.51
Molecular weight167063.34
Publications
PubMed=10731132
PubMed=12537572
PubMed=15297616

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for transcriptional activation in response to heat shock. 
GO - Cellular Component
mediator complex	GO:0016592	ISS:UniProtKB
transcription regulator complex	GO:0005667	ISS:FlyBase

GO - Biological Function
transcription coregulator activity	GO:0003712	ISS:UniProtKB

GO - Biological Process
positive regulation of gene expression	GO:0010628	IBA:GO_Central
regulation of transcription by RNA polymerase II	GO:0006357	ISS:UniProtKB
regulation of transcription, DNA-templated	GO:0006355	ISS:FlyBase









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31596
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     102.03|      16|      16|    1359|    1374|       1
---------------------------------------------------------------------------
 1359- 1374 (35.43/16.22)	QQQPQQPHQQQQQQQQ
 1376- 1391 (33.33/14.85)	QQQQQQQQQQQQQMQQ
 1419- 1434 (33.27/14.81)	QQQMYMQHMQQHQHMQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      93.89|      31|      54|    1004|    1043|       2
---------------------------------------------------------------------------
 1004- 1043 (45.02/48.50)	LHYYERILRdrpalkkKLVGAITSA.........FSEIrpPNWSVSEPY
 1058- 1097 (48.87/29.60)	LSYYMSLIR.......RLADTISGKnvfystdwrFNEF..PNAPTHALY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      95.63|      30|      35|    1278|    1312|       3
---------------------------------------------------------------------------
 1278- 1309 (51.57/42.10)	KPLQYMDqiCDFLYHIKY..IHVGNIIKNESE.AI
 1314- 1346 (44.06/22.18)	RPLLQMR..LRFITHLNLedIHTEKINDNTSNnAI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      35.69|      11|      36|     344|     355|       4
---------------------------------------------------------------------------
  344-  355 (15.49/12.24)	QLIYfVLFQFVS
  378-  388 (20.19/10.22)	QLMW.ILLQFIS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      36.65|      11|      21|     255|     265|       6
---------------------------------------------------------------------------
  255-  265 (21.03/10.88)	PYEPEL....LEEQK
  275-  289 (15.62/ 6.32)	PYSREMvsqmLNLQK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      96.32|      32|      37|     520|     552|       8
---------------------------------------------------------------------------
  520-  552 (51.30/40.98)	GQIPAVTFSVTvLDSLTVHSKM.SL.IHSFVTQML
  559-  592 (45.03/30.60)	GQVPAAALLET.YARLLVYTEIeSLgIKGFLSQLM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.03|      13|      16|     610|     625|      10
---------------------------------------------------------------------------
  613-  625 (25.51/18.25)	YRLHHVPTHYRVQ
  631-  643 (22.52/ 7.21)	HSLSSVPQTNKMQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.68|      14|      20|     200|     213|      11
---------------------------------------------------------------------------
  200-  213 (25.26/15.68)	MTDFVEEFRTTAQM
  222-  235 (27.42/17.63)	MLPIVEHFGYADHM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.68|      21|      26|     943|     967|      13
---------------------------------------------------------------------------
  943-  967 (29.06/28.19)	IPLPVyfSNV..CLRFlpVLD..VVVHRF
  968-  992 (25.62/11.60)	IELTI..TNVhqILGF..ILDhlSILYKF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.37|      16|      75|     662|     677|      18
---------------------------------------------------------------------------
  648-  663 (21.68/12.53)	FESTALRLITSIGSAE
  664-  679 (27.69/18.05)	FQPQFSRYFNDKSPGA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.48|      13|      24|     741|     753|      20
---------------------------------------------------------------------------
  741-  753 (25.52/15.44)	NEYFTQNNQPPEN
  763-  775 (24.96/14.93)	EEYRTWTSMTNEN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.00|      13|      15|    1220|    1232|      21
---------------------------------------------------------------------------
 1220- 1232 (23.63/15.70)	YLKEKLKPCV.RTE
 1237- 1250 (21.36/13.39)	YLCHVFGPFLqRIE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.64|      12|      18|     894|     911|      22
---------------------------------------------------------------------------
  894-  905 (19.24/24.34)	KASELRNRVQEF
  915-  926 (22.40/ 7.65)	KQSNWQDKHLSF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      64.45|      20|      24|     432|     451|      24
---------------------------------------------------------------------------
  432-  451 (38.29/22.97)	PICVWIH..LMKKARVEN..MNIT
  455-  478 (26.16/13.50)	PIALKNHydFLQHLVTANtmMNMT
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31596 with Med23 domain of Kingdom Metazoa
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