<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31594

Description Mediator of RNA polymerase II transcription subunit 23
SequenceMTDKPGSAEDTIEMDDTCSPQFVQEVNRIKSQIKSLVEENHTRKFFRPLTSNIGDETAILRIQFNNMMSKMEEKEKQSLVKELIKMVHHVAEKNSPDRVFVGTNYERVVDQLLRYAHQKGAISTNLCAEGLIMTSDFRLCSRICQEKWKFINDCIPKIDYKGIRNILRYILESQLRRLPYSLSPEQVNEIRIVENVILHIVDRDSNLMPPLITLSEIMRGMPKQALMLPRLTEKLASLSVHFRPIADLSHVCGRGFVYPIPLHPSFYPLTSCWEEHGLNASNTIVQPHHTLPYRPEHTSTYLYTLYMILRQPLGKDSLHPPSKTKTKTNWEMLISVMICESMAEAESLPETEPIPRYQWDNIVNIVIYGITQHLLVPKTFFNVLKNLIKRCKYTRARDEVMWIVFQVVGSLSNLTRLDDAVTEIVELYNELFDGDVVWMGASDHPALFARFLAAAGTWMILEKDFADKMPPANETIKSHIKFIQDGVDNFDSSNTAMLAVLSNIYRTDTKMGKLIVPTLQQMLESIDDTKSLFELSYKRMAVNCFSAFPVEFMEALTFRSKKTLLIQCFQPLRSFSTVRLPSPAVFETVAKICESEDYEMAVKDMEHLAQRSLHVATAADRSSGEHQNVQAKDQCYFLFDFLVYRLPHLHAYSKYSSTVNALSYFTQHIPNNPQNHQIYRLMEQFLMRRWCWAGFHGCITAHTQMFGTSYKDNTMMRHMAYPKTFSVPDQYPFAINPEIFKMAIYSFLRAVKITAQDIAIEKTMFPTIINGFGWPEKSTSYFPKWALDAIKASDTSNAVNTEEILSDVRNTARMHTSLTPNQFVIRYGEDRDPATSHCMLAVLFHFAYNSVDSTYNITSEFYEVMEKKTPKEIVVMGNYLVDYIIADAKTQDCNEKTFKNIAKAAALLVFQFQVLRADRLLLSLIMHPATDEDALICIQIANEFILTPEFQERIRWYHQNVPKKDHFPTEYIKAIVKYHDAFPEFEACELVRSYDSSSNVHMPTYYGCLIERLLPIMDQYLHVALEQQGYKLNPQILQSVSMLYKFHAMPIHFMYSVLFTSHGLMSGPDAKSFVLAFATQIEECHLTEAFEKFNHQKSSREQLIMELIDRMSASLDFILTPPPFVAKDWKIAELSPGAQTLYLACIELMASPHSPETLVAAMINVMQMKPHARPFNIVNLTALLLTALPAAYSNALHDEFVAVFVNGETANLKFEEIVFDNYEESLLLNLPNRARTINVISQQYWLHCSLSLLNFFSHEYVPRILEHVKTEKDLWYTLRLVMPYLRRYYENWDTAKQMRSQRENFGPLHIVKLVFQKLGSMAEEGVEIVYEQHLCDLFYNCKYFFAGDFLRNTAITEFAKLPEKMRDRLKFYVSQSEPTAEQETPPEKEKSPEKEKEQEQEQHVKAHQPLESTPSVSSLPQMQHHLQQAPLLPSHQMMPPPQQHSSSLQHHLQHHTSTHQMMDTSQHQTIQQQSNHPTQQQLQHQIPNMSMHQQMGPQYPGAVFHHPSGPVGHVPMQYGMGHHMQQHPHLPHHQQMPAPMHTMNPMMQNMTPQQQYLYMQQLQQHQQHQQYMQQQQQHHHQHQQQPH
Length1587
PositionTail
OrganismCaenorhabditis elegans
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Nematoda> Chromadorea> Rhabditida> Rhabditina> Rhabditomorpha> Rhabditoidea> Rhabditidae> Peloderinae> Caenorhabditis.
Aromaticity0.10
Grand average of hydropathy-0.347
Instability index49.94
Isoelectric point6.38
Molecular weight183905.17
Publications
PubMed=7557379
PubMed=9851916
PubMed=12130541
PubMed=15268855
PubMed=15790964
PubMed=15901674

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Functions downstream of receptor let-23 and let-60/Ras during vulval induction likely by down-regulating the expression of phosphatase dep-1 and lin- 12/Notch in vulva precursor cell descendants with a primary cell fate (PubMed:7557379, PubMed:15901674). Acts to repress beta-catenin target genes (PubMed:12130541). Required for asymmetric division of T-cells (PubMed:15790964). Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation (PubMed:15268855). 
GO - Cellular Component
mediator complex	GO:0016592	IDA:WormBase
transcription regulator complex	GO:0005667	IBA:GO_Central

GO - Biological Function
GO - Biological Process
defense response to Gram-positive bacterium	GO:0050830	IMUniProtKB
multicellular organism development	GO:0007275	IEA:UniProtKB-KW
positive regulation of gene expression	GO:0010628	IBA:GO_Central
regulation of protein catabolic process	GO:0042176	IMWormBase
regulation of transcription by RNA polymerase II	GO:0006357	IMWormBase









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31594
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     216.18|      23|      23|    1422|    1444|       1
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 1422- 1444 (52.92/24.27)	MQHHLQQAPLLPSHQ.MMPPPQQH
 1448- 1467 (38.02/15.40)	LQHHLQHHT..STHQ.MMDTSQ.H
 1479- 1499 (37.59/15.14)	QQQLQHQIPNMSMHQ.QMGP..QY
 1502- 1518 (25.65/ 8.04)	AVFHH......PSGP.VGHVPMQY
 1520- 1537 (34.45/13.27)	MGHHMQQHPHLPHHQ.QMP.....
 1562- 1578 (27.55/ 9.17)	LQQHQQ.......HQqYMQQQQQH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     474.77|     180|     200|     465|     663|       2
---------------------------------------------------------------------------
  465-  538 (55.32/31.63)	......................................................................................................................................FADKMP..PANETIKSHI.KFIQD..GVDNFDSSNTAMLAVLSNIYRTDTKMGKLIVPTlqqmLESIDDtKSLFELSYK
  539-  738 (277.89/179.18)	RMAVNCF.SAFPVEFMEALTFRSKKTLLIQCF.QPLRsfSTVRLPSPAVFETVAK.ICESEDYEMAVKDMEHLAQrsLHVATAADR...SSGEHQNvQAKDQCYF..LFDFlvyrlphlhAYSKYSSTVNALSyFTQHIPnnPQNHQIYRLMeQFLMRrwCWAGFHGCITAHTQMFGTSYKDNTMMRHMAYPK....TFSVPD.QYPFAINPE
  741-  865 (141.56/72.50)	KMAIYSFlRAVKITAQDIAIEKTMFPTIINGFgWPEK..STSYFPKWALDAIKASdTSNAVNTEEILSDVRNTAR..MHTSLTPNQfviRYGEDRD.PATSHCMLavLFHF.........AYNSVDSTYNITSeFYEVM..........................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     918.46|     305|     805|      92|     463|       3
---------------------------------------------------------------------------
   92-  437 (473.11/384.64)	EKNSPDRVFVGTNYerVVDQLLRYAH.........................................QKGAISTNLCAEGLIMTSDFrlcsricQEKWKFINDCIPKIDY...KGIRNILRYilesqLRRLPYSLSPEQVNEIRIVENVilHIVDRDSNLMPPLITLSE..IMRGMPKQALML.PRLTEKLASL.SVHFRPIADLSHV..CGRGFVYPiPLHPSF.YPLTSCWEEHGLnaSNTIVQPHHtlpyrpEHTSTYLYTLYMILRQPLGKDSLHPPSKTKTKtNWEML.IS......VMIC.ESMAEAESlPET...EPIPRYQW.DNIVNIVIYGITQHLL..VPKTFFNVLknlikrckytraRDEVMwIVFqVVGSLSNLTRLD...DAVTE..IVELYNELFDGDVV
  866- 1240 (445.35/272.96)	EKKTPKEIVVMGNY..LVDYIIADAKtqdcnektfkniakaaallvfqfqvlradrlllslimhpatDEDALICIQIANEFILTPEF.......QERIRWYHQNVPKKDHfptEYIKAIVKY.....HDAFPEFEACELVRSYDSSSNV..HMPTYYGCLIERLLPIMDqyLHVALEQQGYKLnPQILQSVSMLyKFHAMPIHFMYSVlfTSHGLMSG.PDAKSFvLAFATQIEECHL..TEAFEKFNH......QKSSREQLIMELIDRMSASLDFILTPPPFVAK.DWKIAeLSpgaqtlYLACiELMASPHS.PETlvaAMINVMQMkPHARPFNIVNLTALLLtaLPAAYSNAL............HDEFV.AVF.VNGETANLKFEEivfDNYEEslLLNLPNRARTINVI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      70.23|      23|      27|    1360|    1386|       4
---------------------------------------------------------------------------
 1360- 1386 (35.03/31.03)	KLPEKMRDRLKfyvsQSEPTAEQ..ETPP
 1390- 1414 (35.20/20.22)	KSPEKEKEQEQ....EQHVKAHQplESTP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      79.71|      23|      27|    1246|    1272|       5
---------------------------------------------------------------------------
 1246- 1272 (35.06/31.16)	LHCSLSLLnffsHEYVPRILEHVKTEK
 1274- 1296 (44.65/27.75)	LWYTLRLV....MPYLRRYYENWDTAK
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31594 with Med23 domain of Kingdom Metazoa
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