<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31520

Description Isoform 4 of Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEDSERLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFEEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAAPLDKILHGSVGYLTPRSGGHLMNMKYYASPSDLLDDKTASPIILHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYAALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKAKKKKSSRVPPDKPKHQTEDDFQRELFSMDVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPATYPQPVSHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPNSDSPTNHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGEPKFKGSSQADTVDFSIISVAGKALGAADLMEHHSGSQSPLLTTGELGKEKTQKRVKEGNGTGASSGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSLASASTSGKVKSSKSEGSSSSKLSGSMYASQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSSGSHVSGTSSSSGMKSSSGSASSGSVSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNTSGGEFQSKREKSDKDKSKVSASGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESGGDGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSEDGIRPLPEYSTEKHKKHKKEKKKVRDKDRDKKKSHSMKPENWSKSPISSDPTASVTNNPILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1560
PositionMiddle
OrganismMus musculus (Mouse)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Muridae> Murinae> Mus> Mus.
Aromaticity0.04
Grand average of hydropathy-0.660
Instability index53.49
Isoelectric point8.88
Molecular weight167138.71
Publications
PubMed=9325263
PubMed=11471062
PubMed=14500757
PubMed=16141072
PubMed=19468303
PubMed=15489334
PubMed=10882104
PubMed=11266503
PubMed=12037571
PubMed=11867769
PubMed=12709428
PubMed=14636573
PubMed=15150259
PubMed=15340084
PubMed=16137621
PubMed=17132730
PubMed=17525332
PubMed=21183079
PubMed=24043798
PubMed=15528208

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity). 
GO - Cellular Component
chromatin	GO:0000785	ISO:MGI
mediator complex	GO:0016592	IDA:MGI
nucleolus	GO:0005730	ISS:UniProtKB
nucleoplasm	GO:0005654	ISO:MGI
nucleus	GO:0005634	IDA:MGI
protein-DNA complex	GO:0032993	ISO:MGI
ubiquitin ligase complex	GO:0000151	ISO:MGI

GO - Biological Function
chromatin binding	GO:0003682	IDA:MGI
chromatin DNA binding	GO:0031490	ISO:MGI
DNA binding	GO:0003677	IDA:MGI
estrogen receptor binding	GO:0030331	ISO:MGI
LBD domain binding	GO:0050693	ISO:MGI
nuclear hormone receptor binding	GO:0035257	IPI:UniProtKB
nuclear receptor binding	GO:0016922	ISO:MGI
nuclear receptor coactivator activity	GO:0030374	ISS:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	ISO:MGI
promoter-specific chromatin binding	GO:1990841	IDA:MGI
protein-containing complex binding	GO:0044877	IDA:MGI
retinoic acid receptor binding	GO:0042974	ISO:MGI
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	ISO:MGI
thyroid hormone receptor binding	GO:0046966	ISS:UniProtKB
transcription coactivator activity	GO:0003713	IDA:MGI
transcription coregulator activity	GO:0003712	ISS:UniProtKB
transcription corepressor activity	GO:0003714	IMP:UniProtKB
transcription factor binding	GO:0008134	IPI:BHF-UCL
ubiquitin protein ligase activity	GO:0061630	IEA:Ensembl
vitamin D receptor binding	GO:0042809	ISO:MGI

GO - Biological Process
F:transcription corepressor activity	GO:0003714	IMUniProtKB
androgen biosynthetic process	GO:0006702	ISS:UniProtKB
angiogenesis	GO:0001525	IMBHF-UCL
animal organ morphogenesis	GO:0009887	IMMGI
animal organ regeneration	GO:0031100	IMMGI
brain development	GO:0007420	IMMGI
camera-type eye development	GO:0043010	IMMGI
cell morphogenesis	GO:0000902	ISS:UniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	ISS:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	IMMGI
cellular response to steroid hormone stimulus	GO:0071383	ISO:MGI
cellular response to thyroid hormone stimulus	GO:0097067	ISO:MGI
embryonic heart tube development	GO:0035050	IMMGI
embryonic hemopoiesis	GO:0035162	IMMGI
embryonic hindlimb morphogenesis	GO:0035116	IMMGI
embryonic placenta development	GO:0001892	IMMGI
enucleate erythrocyte development	GO:0048822	IMMGI
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IMMGI
erythrocyte development	GO:0048821	IMBHF-UCL
fat cell differentiation	GO:0045444	IMMGI
heart development	GO:0007507	IMMGI
in utero embryonic development	GO:0001701	IMMGI
intracellular steroid hormone receptor signaling pathway	GO:0030518	ISS:UniProtKB
keratinocyte differentiation	GO:0030216	ISS:UniProtKB
lactation	GO:0007595	IMMGI
lens development in camera-type eye	GO:0002088	IMBHF-UCL
liver development	GO:0001889	IMMGI
mammary gland branching involved in pregnancy	GO:0060745	IMMGI
mammary gland branching involved in thelarche	GO:0060744	IMMGI
mammary gland epithelial cell proliferation	GO:0033598	IMMGI
megakaryocyte development	GO:0035855	IMBHF-UCL
monocyte differentiation	GO:0030224	IMMGI
mRNA transcription by RNA polymerase II	GO:0042789	IMUniProtKB
multicellular organism development	GO:0007275	IMMGI
negative regulation of apoptotic process	GO:0043066	IMUniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	ISS:UniProtKB
negative regulation of neuron differentiation	GO:0045665	IMUniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	IMUniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IMMGI
positive regulation of cell population proliferation	GO:0008284	IMMGI
positive regulation of erythrocyte differentiation	GO:0045648	ISS:UniProtKB
positive regulation of G0 to G1 transition	GO:0070318	IMMGI
positive regulation of gene expression	GO:0010628	ISS:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	IMMGI
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IMMGI
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IMMGI
positive regulation of keratinocyte differentiation	GO:0045618	ISS:UniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:MGI
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	ISS:UniProtKB
positive regulation of transcription, DNA-templated	GO:0045893	ISO:MGI
protein import into nucleus	GO:0006606	IMMGI
protein ubiquitination	GO:0016567	ISO:MGI
regulation of RNA biosynthetic process	GO:2001141	ISS:UniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of transcription, DNA-templated	GO:0006355	IDA:MGI
regulation of vitamin D receptor signaling pathway	GO:0070562	IMMGI
retinal pigment epithelium development	GO:0003406	IMBHF-UCL
thyroid hormone generation	GO:0006590	IMMGI
thyroid hormone mediated signaling pathway	GO:0002154	ISS:UniProtKB
transcription by RNA polymerase II	GO:0006366	TAS:MGI
ventricular trabecula myocardium morphogenesis	GO:0003222	IMBHF-UCL









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31520
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     325.95|      50|      50|    1079|    1128|       1
---------------------------------------------------------------------------
  970- 1015 (43.41/ 9.15)	KrvKEGNGTG..............ASS...GSGPGSD.SKPGKRSRTPSNDGKSK..DKP..........PKRKKA
 1016- 1075 (39.09/ 7.30)	D..TEGKSPSH.....ssS.NRPFTPP...T..STGG.SKSPGSSGRSQTPPGVA..TPPipkitiqipkGTVMVG
 1076- 1119 (65.71/18.72)	K..P.............sS.HSQYTSS...GSVSSSG.SKSHHSHSSSSSSLASA..STS..........GKVKSS
 1120- 1158 (52.53/13.07)	K..SEGSSSSK......lS.GSMYASQ...GSSGSSQ.SK.......NSS.......QTG..........GKPGSS
 1165- 1221 (41.46/ 8.31)	L..SSGSSSTK...mkpqG.KPSSLMN...PSISKPNiSPSHSRPPGGSDKLASPmkPVP..........GTPPSS
 1249- 1299 (43.44/ 9.16)	S..GSASSGSVsqktppaS.NSCTPSS...SSFSSSG.S......SMSSSQNQHG..SSK..........GKSPSR
 1419- 1466 (40.31/ 7.82)	G..GDG.............lRPQIASSknyGSPLISG.STPKHERGSPSHSKSPA..YTP..........QNVDSE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     348.28|      51|      52|     699|     749|       2
---------------------------------------------------------------------------
  548-  584 (36.14/12.82)	............YGM.T....TGN...NP........MSG..TT.TPTNTFPG...GPITTLFNM.SMSI.......KD
  585-  633 (61.15/28.24)	RH..ESVGHGEDFS.KV.....SQ...NP........ILTslLQ.ITGNGGST...IGSSPTPPHHTPPP.......VS
  650-  700 (41.48/16.12)	KDNPAQDFSTLYGSSPL....ERQ...NSssgsprmeMCS..GS.NKAKKKKS...SRVPP...............dKP
  701-  755 (85.13/43.03)	KHQTEDDFQRELFSMDV....DSQ...NP........MFD..VS.MTADALDT...PHITPAPSQCSTPP...atypQP
  756-  806 (52.09/22.65)	VSHPQPSIQRMVRLSSS....DSI...GP..........D..VTdILSDIAEE...ASKLPSTSDDC.PP.....igTP
  814-  870 (44.85/18.20)	GHS.....QSALFDSDVfqtnNNE...NP........YTD.pAD.LIADAAGS...PN.SDSPTNHFFPDgvdfnpdLL
  871-  911 (27.44/ 7.46)	NSQSQSGFGEEYF..DE....SSQsgdND........DFK...G.FASQALNTlgmPML....................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     163.25|      38|      48|     146|     183|       3
---------------------------------------------------------------------------
  146-  182 (57.14/37.24)	................N.....FE.EFSKHL...KGLV.NLYNL..........P..GDNKLKT.KMYLALQSLEQ
  183-  233 (43.31/26.10)	D...lskmaimywkatN.....AA.PLDKIL...HGSV.G.YLT..........PrsGGHLMNM.KYYASPSDLLD
  234-  296 (31.51/16.60)	DktaspiilheknvprS.....LGmNASVTI...EGTS.AMYKLpiaplimgshP..ADNKW.T.PSFSAVTSANS
  298-  342 (31.29/16.41)	D................lpacfFL.KFPQPIpvsKAFVqKLQNC..........T..GIPLFETpPTYLPLYEL..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     203.41|      43|      98|    1368|    1410|       4
---------------------------------------------------------------------------
 1332- 1370 (60.25/26.66)	GAST.NSSNHPMSSKHNTSGGEFQ...SKRE.KSDKDKSKVSAS
 1371- 1410 (58.07/25.38)	GGSV.DSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKV...
 1470- 1512 (55.77/24.02)	GSSIaERSYQNSPSSEDGIRPLPEYSTEKHKKHKKE.KKKVRDK
 1513- 1539 (29.31/ 8.41)	.....DRDKKKSHS..MKPENWSKSPISSDPTAS..........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     117.73|      38|     340|     101|     144|       5
---------------------------------------------------------------------------
  101-  144 (56.53/51.35)	CYITSDMFYVEVQlDPAGQ..LC...DVKVAHHgenpVSCpELVQQLRE
  443-  485 (61.20/35.64)	CPLSESRFSVSFQ.HPVNDslVCvvmDVQDSTH....VSC.KLYKGLSD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31520 with Med1 domain of Kingdom Metazoa
Unable to open file!