<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31518

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1581
PositionMiddle
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.04
Grand average of hydropathy-0.677
Instability index53.55
Isoelectric point8.88
Molecular weight168476.15
Publications
PubMed=9444950
PubMed=9653119
PubMed=15489334
PubMed=10235267
PubMed=10733574
PubMed=10235266
PubMed=7776974
PubMed=10024883
PubMed=10406464
PubMed=10478845
PubMed=10770935
PubMed=11303023
PubMed=12218053
PubMed=12037571
PubMed=11867769
PubMed=12034878
PubMed=12556447
PubMed=14636573
PubMed=15471764
PubMed=15175163
PubMed=15340084
PubMed=15989967
PubMed=16314496
PubMed=17081983
PubMed=16574658
PubMed=17000779
PubMed=16964243
PubMed=17525332
PubMed=18691976
PubMed=18669648
PubMed=19413330
PubMed=19369195
PubMed=19786558
PubMed=19690332
PubMed=19608861
PubMed=20068231
PubMed=21269460
PubMed=21406692
PubMed=23186163
PubMed=24245781
PubMed=24275569
PubMed=29997176

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1- mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). 
ECO:0000269	PubMed:10406464
ECO:0000269	PubMed:11867769
ECO:0000269	PubMed:12037571
ECO:0000269	PubMed:12218053
ECO:0000269	PubMed:12556447
ECO:0000269	PubMed:14636573
ECO:0000269	PubMed:15340084
ECO:0000269	PubMed:15471764
ECO:0000269	PubMed:15989967
ECO:0000269	PubMed:16574658
ECO:0000269	PubMed:24245781
ECO:0000269	PubMed:9653119
GO - Cellular Component
chromatin	GO:0000785	IEA:Ensembl
mediator complex	GO:0016592	IDA:UniProtKB
membrane	GO:0016020	HDA:UniProtKB
nucleolus	GO:0005730	IDA:UniProtKB
nucleoplasm	GO:0005654	IDA:HPA
nucleus	GO:0005634	IDA:UniProtKB
protein-DNA complex	GO:0032993	IEA:Ensembl
ubiquitin ligase complex	GO:0000151	IEA:Ensembl
GO - Biological Function
chromatin binding	GO:0003682	IMP:UniProtKB
chromatin DNA binding	GO:0031490	IEA:Ensembl
estrogen receptor binding	GO:0030331	IPI:UniProtKB
LBD domain binding	GO:0050693	IPI:UniProtKB
nuclear hormone receptor binding	GO:0035257	IPI:UniProtKB
nuclear receptor binding	GO:0016922	IDA:UniProtKB
nuclear receptor coactivator activity	GO:0030374	IMP:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	IPI:UniProtKB
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IPI:UniProtKB
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IDA:UniProtKB
transcription coactivator activity	GO:0003713	IDA:UniProtKB
transcription coregulator activity	GO:0003712	IDA:UniProtKB
transcription corepressor activity	GO:0003714	ISS:UniProtKB
transcription factor binding	GO:0008134	IPI:UniProtKB
ubiquitin protein ligase activity	GO:0061630	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IPI:UniProtKB
GO - Biological Process
F:chromatin binding	GO:0003682	IMUniProtKB
F:nuclear receptor coactivator activity	GO:0030374	IMUniProtKB
androgen biosynthetic process	GO:0006702	IMUniProtKB
angiogenesis	GO:0001525	ISS:UniProtKB
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	IMUniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	IDA:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to steroid hormone stimulus	GO:0071383	IDA:UniProtKB
cellular response to thyroid hormone stimulus	GO:0097067	IDA:UniProtKB
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
erythrocyte development	GO:0048821	ISS:UniProtKB
fat cell differentiation	GO:0045444	IDA:MGI
intracellular steroid hormone receptor signaling pathway	GO:0030518	IDA:UniProtKB
keratinocyte differentiation	GO:0030216	IMUniProtKB
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	ISS:UniProtKB
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	ISS:UniProtKB
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	ISS:UniProtKB
negative regulation of apoptotic process	GO:0043066	ISS:UniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	IMUniProtKB
negative regulation of neuron differentiation	GO:0045665	ISS:UniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	ISS:UniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IMUniProtKB
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IDA:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IMUniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:UniProtKB
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IDA:UniProtKB
positive regulation of transcription, DNA-templated	GO:0045893	IDA:UniProtKB
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of lipid metabolic process	GO:0019216	TAS:Reactome
regulation of RNA biosynthetic process	GO:2001141	IMUniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IMUniProtKB
transcription initiation from RNA polymerase II promoter	GO:0006367	TAS:Reactome
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl

Interaction

Binary Interactions
[Q15648<-->P03372: ESR1]	NbExp=3	EBI-394459,EBI-78473
[Q15648<-->P15976: GATA1]	NbExp=6	EBI-394459,EBI-3909284
[Q15648<-->Q13503: MED21]	NbExp=3	EBI-394459,EBI-394678
[Q15648<-->O43513: MED7]	NbExp=6	EBI-394459,EBI-394632
[Q15648<-->P10276: RARA]	NbExp=6	EBI-394459,EBI-413374
[Q15648<-->P19793: RXRA]	NbExp=6	EBI-394459,EBI-78598
[Q15648<-->P10827: THRA]	NbExp=4	EBI-394459,EBI-286285
[Q15648<-->P11473: VDR]	NbExp=4	EBI-394459,EBI-286357

Repeat regions

Repeats

>MDP31518
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|    1091.36|     155|     155|    1034|    1188|       1
---------------------------------------------------------------------------
  542-  710 (161.34/37.98)	P....................A.S.....SPGYGMTTGNN....PmsGTTTPtntfPGGPITtlfnmsMSIKDRHE..SVGHGEDFSkvsqnpiltsllQI.......T..GNGgsTIGSSPTPP........HHTPPPVSSM...AGNTKNHpmlmnlLKDNP....AQD.......F...STLY......GSS......PLERQN....S..................SSG....SP...RMEICSGSNKTKKKkssrlPPEK.PK.HQTEDDFQR
  711-  875 (162.42/38.29)	ElfsmdvdsqnpifdvnmtadT.LDTPHITP.APSQCSTP....P..TTYPQ....PVPH...........PQPSIqrMVRLSSSDS............IG.......P..DVT..DILSDIAEE........ASKLPSTSDD...CPAIGTP......LRDSS....SSGHSQSTLfD...SDVF..QTNNNEN......PYTDPAdliaD..................AAGSPSSDSPT.NHFFHDG............VDFN.P..DLLN.SQSQ
  881- 1033 (149.32/34.58)	E....................Y.FDESSQS....GDNDDF....K..GFASQ....ALN..T......LGVP......MLGGDNGET............KFkgnnqadT..VDF..SIISVAGKAlapadlmeHHSGSQGPLL...TTGDLGK......EKTQKrvkeGNGTSNSTL.S...GPGLDSKPGKRSR......TPSNDG....K..................SKDKP...P.K.RK.........KAD.....TEGKsPShSSSNRPFTP
 1034- 1188 (261.49/66.32)	P....................T.STGGSKSPGSAGRSQTP....P..GVATP....PIPKIT......IQIPKGTV..MVGKPSSHS............QY.......T..SSG..SVSSSGSKS........HHSHSSSSSS...SASTSGK......MKSSK....SEGSSSSKL.S...SSMYSSQGSSGSS......QSKNSS....Q..................SGGKPGSSPIT.KHGLSSGSSSTKMK.....PQGK.PS.SLMNPSLSK
 1189- 1348 (175.41/41.96)	P....................NiSPSHSRPPGGSDKLASPmkpvP..G..TP....PSSK...........AKSPI..SSGSGGSH.........................MSG..TSSSSGMKS........SSGLGSSGSL...SQKTPP.........SSN....SCTASSSSF.SssgSSMSSSQNQHGSS......KGKSPS....RnkkpsltavidklkhgvvTSGPGGEDPLDgQMGVSTNSSSHPM............S.SKHN..MSG
 1364- 1523 (181.38/43.65)	K....................V.STSGS.SVDSSKKTSES....K..NVGST....GVAKII......ISKHDG......GSPSIKA............KV.......TlqKPG..ESSGEGLRP........QMASSKNYGSpliSGSTPKH......ERGSP....SH.SKSPAY.T...PQNLDSESESGSSiaeksyQNSPSS....D..................DGIRPLPEYST.EKHKKHKKEKKKVK.....DKDR.DR.DRDKDRDKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     207.12|      48|      48|     146|     193|       2
---------------------------------------------------------------------------
  146-  193 (81.51/51.02)	NFD.EFSKHLKGLVNLYNL..........P..GDNKLKTKMYLALQSLEQ.DLSKMAIMYWK
  196-  244 (70.55/43.02)	NAG.PLDKILHGSVG.YLT..........PrsGGHLMNLKYYVSPSDLLD.DKTASPIILHE
  250-  306 (55.06/31.73)	SLGmNASVTIEGTSAVYKLpiaplimgshP..VDNKW.TPSFSSITSANSvDLP..ACFFLK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     241.33|      49|      55|     376|     424|       3
---------------------------------------------------------------------------
  328-  373 (49.69/28.66)	.....P..LF.ETQPTYAPLYELITqFelsKDPDPIPLNHNM.RF...YAALPGQqhC
  376-  424 (84.03/54.05)	LNKDAP..LP.DGRSLQGTLVSKIT.F...QHPGRVPLILNLIRHQVAYNTLIGS..C
  430-  477 (63.57/38.92)	LKEDSPglLQfEVCPLSESRFS.VS.F...QHPVNDSLV..CVVMDVQDSTHV.S..C
  479-  514 (44.03/24.47)	LYKG....LS.DALICTDDFIAKVV.....QRCMSIPVTMRAIRRK............
---------------------------------------------------------------------------

Associated diseases

Disease
Med1    osteosarcoma    PMID:24969180
Med1    breast cancer   PMID:22345290
Med1    lung cancer PMID:22342682
Med1    cancer  PMID:16574658
Med1    cancer cell lines       PMID:19521987
Med1    cancer treatment        PMID:27340176
Med1    breast cancer   PMID:17344214
metabolic diseases	PMID:24801167 PMID:20479251
neurodegenerative diseases	PMID:25564654
muscle diseases	PMID:20479251
fatty liver disease	PMID:33734012


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31518 with Med1 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
2) FNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFN
3) KNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGP
4) QSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADT
937
576
538
873
1581
866
571
928










Molecular Recognition Features






MoRF SequenceStartStop




1) FGEEYFD
2) IRPLPEYSTEKHKKHKKEKKKVKDKDRDR
3) KITIQIPK
4) KKPSLTAVIDKLKHGV
5) RLSPDFMIGEEDDDLMDV
6) SIKAKVTLQK
7) VAKIIISKH
878
1486
1062
1299
1559
1403
1389
884
1514
1069
1314
1576
1412
1397