<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31518

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1581
PositionMiddle
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.04
Grand average of hydropathy-0.677
Instability index53.55
Isoelectric point8.88
Molecular weight168476.15
Publications
PubMed=9444950
PubMed=9653119
PubMed=15489334
PubMed=10235267
PubMed=10733574
PubMed=10235266
PubMed=7776974
PubMed=10024883
PubMed=10406464
PubMed=10478845
PubMed=10770935
PubMed=11303023
PubMed=12218053
PubMed=12037571
PubMed=11867769
PubMed=12034878
PubMed=12556447
PubMed=14636573
PubMed=15471764
PubMed=15175163
PubMed=15340084
PubMed=15989967
PubMed=16314496
PubMed=17081983
PubMed=16574658
PubMed=17000779
PubMed=16964243
PubMed=17525332
PubMed=18691976
PubMed=18669648
PubMed=19413330
PubMed=19369195
PubMed=19786558
PubMed=19690332
PubMed=19608861
PubMed=20068231
PubMed=21269460
PubMed=21406692
PubMed=23186163
PubMed=24245781
PubMed=24275569
PubMed=29997176

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1- mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). 
GO - Cellular Component
chromatin	GO:0000785	IEA:Ensembl
mediator complex	GO:0016592	IDA:UniProtKB
membrane	GO:0016020	HDA:UniProtKB
nucleolus	GO:0005730	IDA:UniProtKB
nucleoplasm	GO:0005654	IDA:HPA
nucleus	GO:0005634	IDA:UniProtKB
protein-DNA complex	GO:0032993	IEA:Ensembl
ubiquitin ligase complex	GO:0000151	IEA:Ensembl

GO - Biological Function
chromatin binding	GO:0003682	IMP:UniProtKB
chromatin DNA binding	GO:0031490	IEA:Ensembl
estrogen receptor binding	GO:0030331	IPI:UniProtKB
LBD domain binding	GO:0050693	IPI:UniProtKB
nuclear hormone receptor binding	GO:0035257	IPI:UniProtKB
nuclear receptor binding	GO:0016922	IDA:UniProtKB
nuclear receptor coactivator activity	GO:0030374	IMP:UniProtKB
peroxisome proliferator activated receptor binding	GO:0042975	IPI:UniProtKB
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IPI:UniProtKB
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IDA:UniProtKB
transcription coactivator activity	GO:0003713	IDA:UniProtKB
transcription coregulator activity	GO:0003712	IDA:UniProtKB
transcription corepressor activity	GO:0003714	ISS:UniProtKB
transcription factor binding	GO:0008134	IPI:UniProtKB
ubiquitin protein ligase activity	GO:0061630	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IPI:UniProtKB

GO - Biological Process
F:chromatin binding	GO:0003682	IMUniProtKB
F:nuclear receptor coactivator activity	GO:0030374	IMUniProtKB
androgen biosynthetic process	GO:0006702	IMUniProtKB
angiogenesis	GO:0001525	ISS:UniProtKB
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	IMUniProtKB
cellular response to epidermal growth factor stimulus	GO:0071364	IDA:UniProtKB
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to steroid hormone stimulus	GO:0071383	IDA:UniProtKB
cellular response to thyroid hormone stimulus	GO:0097067	IDA:UniProtKB
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
erythrocyte development	GO:0048821	ISS:UniProtKB
fat cell differentiation	GO:0045444	IDA:MGI
intracellular steroid hormone receptor signaling pathway	GO:0030518	IDA:UniProtKB
keratinocyte differentiation	GO:0030216	IMUniProtKB
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	ISS:UniProtKB
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	ISS:UniProtKB
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	ISS:UniProtKB
negative regulation of apoptotic process	GO:0043066	ISS:UniProtKB
negative regulation of keratinocyte proliferation	GO:0010839	IMUniProtKB
negative regulation of neuron differentiation	GO:0045665	ISS:UniProtKB
negative regulation of transcription by RNA polymerase II	GO:0000122	ISS:UniProtKB
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IMUniProtKB
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IDA:UniProtKB
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IMUniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:UniProtKB
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IDA:UniProtKB
positive regulation of transcription, DNA-templated	GO:0045893	IDA:UniProtKB
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of lipid metabolic process	GO:0019216	TAS:Reactome
regulation of RNA biosynthetic process	GO:2001141	IMUniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IMUniProtKB
transcription initiation from RNA polymerase II promoter	GO:0006367	TAS:Reactome
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31518
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|    1091.36|     155|     155|    1034|    1188|       1
---------------------------------------------------------------------------
  542-  710 (161.34/37.98)	P....................A.S.....SPGYGMTTGNN....PmsGTTTPtntfPGGPITtlfnmsMSIKDRHE..SVGHGEDFSkvsqnpiltsllQI.......T..GNGgsTIGSSPTPP........HHTPPPVSSM...AGNTKNHpmlmnlLKDNP....AQD.......F...STLY......GSS......PLERQN....S..................SSG....SP...RMEICSGSNKTKKKkssrlPPEK.PK.HQTEDDFQR
  711-  875 (162.42/38.29)	ElfsmdvdsqnpifdvnmtadT.LDTPHITP.APSQCSTP....P..TTYPQ....PVPH...........PQPSIqrMVRLSSSDS............IG.......P..DVT..DILSDIAEE........ASKLPSTSDD...CPAIGTP......LRDSS....SSGHSQSTLfD...SDVF..QTNNNEN......PYTDPAdliaD..................AAGSPSSDSPT.NHFFHDG............VDFN.P..DLLN.SQSQ
  881- 1033 (149.32/34.58)	E....................Y.FDESSQS....GDNDDF....K..GFASQ....ALN..T......LGVP......MLGGDNGET............KFkgnnqadT..VDF..SIISVAGKAlapadlmeHHSGSQGPLL...TTGDLGK......EKTQKrvkeGNGTSNSTL.S...GPGLDSKPGKRSR......TPSNDG....K..................SKDKP...P.K.RK.........KAD.....TEGKsPShSSSNRPFTP
 1034- 1188 (261.49/66.32)	P....................T.STGGSKSPGSAGRSQTP....P..GVATP....PIPKIT......IQIPKGTV..MVGKPSSHS............QY.......T..SSG..SVSSSGSKS........HHSHSSSSSS...SASTSGK......MKSSK....SEGSSSSKL.S...SSMYSSQGSSGSS......QSKNSS....Q..................SGGKPGSSPIT.KHGLSSGSSSTKMK.....PQGK.PS.SLMNPSLSK
 1189- 1348 (175.41/41.96)	P....................NiSPSHSRPPGGSDKLASPmkpvP..G..TP....PSSK...........AKSPI..SSGSGGSH.........................MSG..TSSSSGMKS........SSGLGSSGSL...SQKTPP.........SSN....SCTASSSSF.SssgSSMSSSQNQHGSS......KGKSPS....RnkkpsltavidklkhgvvTSGPGGEDPLDgQMGVSTNSSSHPM............S.SKHN..MSG
 1364- 1523 (181.38/43.65)	K....................V.STSGS.SVDSSKKTSES....K..NVGST....GVAKII......ISKHDG......GSPSIKA............KV.......TlqKPG..ESSGEGLRP........QMASSKNYGSpliSGSTPKH......ERGSP....SH.SKSPAY.T...PQNLDSESESGSSiaeksyQNSPSS....D..................DGIRPLPEYST.EKHKKHKKEKKKVK.....DKDR.DR.DRDKDRDKK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     207.12|      48|      48|     146|     193|       2
---------------------------------------------------------------------------
  146-  193 (81.51/51.02)	NFD.EFSKHLKGLVNLYNL..........P..GDNKLKTKMYLALQSLEQ.DLSKMAIMYWK
  196-  244 (70.55/43.02)	NAG.PLDKILHGSVG.YLT..........PrsGGHLMNLKYYVSPSDLLD.DKTASPIILHE
  250-  306 (55.06/31.73)	SLGmNASVTIEGTSAVYKLpiaplimgshP..VDNKW.TPSFSSITSANSvDLP..ACFFLK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     241.33|      49|      55|     376|     424|       3
---------------------------------------------------------------------------
  328-  373 (49.69/28.66)	.....P..LF.ETQPTYAPLYELITqFelsKDPDPIPLNHNM.RF...YAALPGQqhC
  376-  424 (84.03/54.05)	LNKDAP..LP.DGRSLQGTLVSKIT.F...QHPGRVPLILNLIRHQVAYNTLIGS..C
  430-  477 (63.57/38.92)	LKEDSPglLQfEVCPLSESRFS.VS.F...QHPVNDSLV..CVVMDVQDSTHV.S..C
  479-  514 (44.03/24.47)	LYKG....LS.DALICTDDFIAKVV.....QRCMSIPVTMRAIRRK............
---------------------------------------------------------------------------











Associated diseases
Disease
Med1    osteosarcoma    PMID:24969180
Med1    breast cancer   PMID:22345290
Med1    lung cancer PMID:22342682
Med1    cancer  PMID:16574658
Med1    cancer cell lines       PMID:19521987
Med1    cancer treatment        PMID:27340176
Med1    breast cancer   PMID:17344214
metabolic diseases	PMID:24801167 PMID:20479251
neurodegenerative diseases	PMID:25564654
muscle diseases	PMID:20479251
fatty liver disease	PMID:33734012



























Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31518 with Med1 domain of Kingdom Metazoa
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