<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31517

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMSGSNAKSSGTGFGSHIPSIEEKNKQIQQETMMEKLRAKYRNKPKSYEEIKKSVRMYFIEKHYPLDPLCKATLQSALDKLQHYIKVTSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDAAGSLSDVKVHHECKIEQQSSELVACLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDSYSLMTSSSVGLVLPRRGGHPMRLTYFCPPLHLPEGDPKLASGDFTIDQVMRSSYGLSATINLEGSSANKLQTLPTVTLVRDAQTGLEVPTYAQLNQNNSLLMPATFVLRLNKPMPVCLESLKALGLPGLDSVATPPGPPTTVLNLIVQTASKQAIKNTQRGLYVNLPKETHCYFFTDNRKLQGTLVSSLPFTEPAQVPRIVAFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFNQITVELQHPYEESLATVDFLLEDGQPTCSVYCLTNEYELLSQKLTRTARKVVSIPMVIYKLLKCWDEEHEFKLHGAIGPGSGSGAIGGGGMSGGGPVSGVGNNFSQFSMDTPTPSDGSLPGGGFANINNLKMDAKSRSLADAFAASTSAAAAIAGLINLKRETDPQSGSSASGTTVSGSSSSSGSAKTSDHDIADKYKNIWKDKTPNLKHCVSITPIPGDGKSGSAGGVSGVEVQRTGGIEIIPLNAQAAIAGGGVTASSSATPTTITITPITGKDPSKDSTKKSTAASAGVGVAAKRPHESSTSSSSTSGCSGSGSSMSSSASSGSSDTQKEKKRKKKRDDSPMGPPEKIYSRQNSPAGGADASATGGVVRKFSSPSSSPKAGGGGQGLMAGVPTARPSPKHSPVYSSPKHNTASNSPKSPFGTHSPKHGSSGKPSMSTLKSAATAATILSPKGDKSSSAVGNTSSGPSASSGSSGATGLVRSFASVGAPPPPPPIPPLASSSGSISSSQSLKKEKTSSASGSSSTSSSATAGVASGGGISPASVAAAVAALKSSQQQMKSVASLSHLAAGGGLGSYAAPSGAGASGAAAVVVGAGAGAGAGASGLELSALRKGMAGGAVSLMTSTAALAPTIPAPTTTVAAGSAASLVSPVSAVVGQGQETAGAAAAATLATATILQQQPQPGAAPTSSCLTTSGGSSDSAGSINPAGASTEYMVKPSSQEGLKLTINKTGSSKSSGTGSGSSSSSGLQAKAKSSSSGATSFAGSTGSTKKQHTGLKPGVNSGPASKKATAAVSSATASSSKHFFQKANSSGNLSSKLSGSGSGGGIPLTKSNSTNSFQEHNAPRRRPSMGALASGSSGGGSGQRKLGSASGGGSGSSGSVSPALSGSMSQPPPRFDHHTDMMTILQYASPTMAASMEGFIKGLHNKFQIPKLSQRGSGGNTTSGRSTPSGSSEPALAGTSSSILGPIASSTGLTEPEAKPPVPPSQSGNEGLLNLSSTAGTPSADGIDEELLASLAGE
Length1475
PositionMiddle
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.04
Grand average of hydropathy-0.283
Instability index52.34
Isoelectric point9.39
Molecular weight149529.69
Publications
PubMed=10731132
PubMed=12537572
PubMed=16751183
PubMed=18327897

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for activated transcription of the MtnA, MtnB and MtnD genes. 
GO - Cellular Component
mediator complex	GO:0016592	IMP:FlyBase
nucleus	GO:0005634	IFlyBase

GO - Biological Function
transcription coregulator activity	GO:0003712	IMP:UniProtKB

GO - Biological Process
C:mediator complex	GO:0016592	IMFlyBase
F:transcription coregulator activity	GO:0003712	IMUniProtKB
positive regulation of transcription by RNA polymerase II	GO:0045944	IGI:FlyBase
regulation of transcription by RNA polymerase II	GO:0006357	IMUniProtKB









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31517
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     947.77|     139|     140|     519|     657|       1
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  519-  642 (201.76/59.56)	...................WDE.EHE....FK..L.....H...GAI....GPGSGS.GAIGG...GGMS...............G..GGP.VSG....V......GNNFSQFSMDTPTPSDG.............SLPGGG....F........ANINNLKMDAKSRSL.ADAFAAST.SAAAAIAGL...I.N....LKRETD.PQ.SGSSAS.....GTT.VSGSS.SSSG..SAKT
  643-  781 (132.79/36.30)	SDHDIADKYKNIWKD...........ktpnLK..H.....C...VSI....TP.......IPG...DGKS...............GsaGG..VSG....VevqrtgGIEI......IPLNAQA.............AIAGGG....VtasssatpTTITITPITGKDPS..KDSTKKST.AASAGV.G....V.A....AKRPHE.SStSSSSTS.....GCS.GSGSSmSSSA..SSGS
  782-  879 (104.07/26.61)	SDTQKEKKRKK.KRD................................dspmGPPEKI.YS.RQ...NSPA...............G..GAD.ASA....T......GGVVRKFSSPSSSPKAG................GGG.qglM........AGVPTARPSPKHSPV....YSSP......................KHNTA.SN.SPKSPF.....GT..................
  881-  987 (96.18/23.95)	...................................................SPKHGS.S..GK...PSMStlksaataatilspkG..DKS.SSA....V......GNTSS.....GPSASSG.............SSGATGlvrsF........ASVGAPPPPPPIPPL.A........SSSGSISSS...Q.S....LKKE......KTSSA...........SGSS.STSS..SATA
  996- 1123 (116.90/30.94)	SPASVAAAVAAL.KS.......sQQQ....MK..SvaslsH...LAA....GGGLGSyAAPSG...AGAS...............G..AAAvVVG....A......G.......A........................GAG.............AGASGLELSALRKGM.AGGAVSLM.TSTAALAPT...I.P....APTTT..VA.AGSAASlvspvSAV.VGQGQ.ETAG..AAAA
 1124- 1274 (117.59/31.18)	ATLATA....TILQQ.................qpQ.....P...GA.....APTSSC.LTTSG...GSSD...............S..AGS.INP....A......GA..STEYMVKPSSQEGlkltinktgssksSGTGSG....S........SSSSGLQAKAKSSSSgATSFAGSTgSTKKQHTGLkpgV.NsgpaSKKATA.AV.SSATAS.....SSKhFFQKA.NSSGnlSSKL
 1275- 1407 (126.85/34.30)	SGSGSGGGIPLTKSNstnsFQE.HNA....PR..R.....RpsmGAL....ASGSSG.GGSGQrklGSAS...............G..GG...SGssgsV......SPALSG.SMSQPPPR.........................F................DHHTDMM.TILQYASP.TMAASMEGF...IkG....LHNKFQiPKlSQRGSG.....GNT.TSGRS.TPSG......
 1408- 1474 (51.64/ 8.93)	......................................................SSE.PALAG...TSSS...............I..LGP.IAS...........STGLTEPEAKPPVPPSQ.............S....G....N........EGLLNLSSTAGTPS..ADGI..DE.ELLASLAG....................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     187.11|      58|      69|     177|     237|       2
---------------------------------------------------------------------------
  177-  237 (90.61/68.17)	NAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDsySLMTSSSVGLVLPRRGGHPMRlTY
  246-  303 (96.50/61.56)	EGDPKLASGDFTIDQVMRSSYGLSATINLEGSSAN..KLQTLPTVTLVRDAQTGLEVP.TY
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31517 with Med1 domain of Kingdom Metazoa
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