<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31429

Description Mediator of RNA polymerase II transcription subunit 15
SequenceMSAAPVQDKDTLSNAERAKNVNGLLQVLMDINTLNGGSSDTADKIRIHAKNFEAALFAKSSSKKEYMDSMNEKVAVMRNTYNTRKNAVTAAAANNNIKPVEQHHINNLKNSGNSANNMNVNMNLNPQMFLNQQAQARQQVAQQLRNQQQQQQQQQQQQRRQLTPQQQQLVNQMKVAPIPKQLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYKIHQQLLFKARLQQQQAQAQAQANNNNNGLPQNGNINNNINIPQQQQMQPPNSSANNNPLQQQSSQNTVPNVLNQINQIFSPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNNANNNGSNLQRAQNVPMNIIQQQQQQNTNNNDTIATSATPNAAAFSQQQNASSKLYQMQQQQQAQAQAQAQAQAQAQAQAQAQAAQAAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAHAQHQPSQQPQQAQQQPNPLHGLTPTAKDVEVIKQLSLDASKTNLRLTDVTNSLSNEEKEKIKMKLKQGQKLFVQVSNFAPQVYIITKNENFLKEVFQLRIFVKEILEKCAEGIFVVKLDTVDRLIIKYQKYWESMRIQILRRQAILRQQQQMANNNGNPGTTSTGNNNNIATQQNMQQSLQQMQHLQQLKMQQQQQQQQQQQQQQQQQQQQQQQHIYPSSTPGVANYSAMANAPGNNIPYMNHKNTSSMDFLNSMENTPKVPVSAAATPSLNKTINGKVNGRTKSNTIPVTSIPSTNKKLSISNAASQQPTPRSASNTAKSTPNTNPSPLKTQTKNGTPNPNNMKTVQSPMGAQPSYNSAIIENAFRKEELLLKDLEIRKLEISSRFKHRQEIFKDSPMDLFMSTLGDCLGIKDEEMLTSCTIPKAVVDHINGSGKRKPTKAAQRARDQDSIDISIKDNKLVMKSKFNKSNRSYSIALSNVAAIFKGIGGNFKDLSTLVHSSSPSTSSNMDVGNPRKRKASVLEISPQDSIASVLSPDSNIMSDSKKIKVDSPDDPFMTKSGATTSEKQEVTNEAPFLTSGTSSEQFNVWDWNNWTSAT
Length1081
PositionTail
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces.
Aromaticity0.04
Grand average of hydropathy-0.870
Instability index51.29
Isoelectric point9.81
Molecular weight120307.29
Publications
PubMed=3062377
PubMed=1406662
PubMed=9169874
PubMed=24374639
PubMed=8789261
PubMed=2005915
PubMed=8187178
PubMed=11478912
PubMed=14562095
PubMed=14562106
PubMed=15175151
PubMed=15477388
PubMed=16076843
PubMed=16109375
PubMed=15665377
PubMed=16263706
PubMed=16630888
PubMed=16429153
PubMed=17192271
PubMed=17330950
PubMed=17287358
PubMed=18407956
PubMed=19779198
PubMed=19940160
PubMed=22814378
PubMed=12191485

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C- terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription. 
ECO:0000269	PubMed:16076843
ECO:0000269	PubMed:16109375
ECO:0000269	PubMed:16263706
GO - Cellular Component
core mediator complex	GO:0070847	IDA:SGD
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
RNA polymerase II activating transcription factor binding	GO:0001102	IDA:SGD
TFIIE-class transcription factor complex binding	GO:0001095	IDA:SGD
TFIIH-class transcription factor complex binding	GO:0001097	IDA:SGD
transcription coactivator activity	GO:0003713	IDA:SGD
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IMSGD
positive regulation of invasive growth in response to glucose limitation	GO:2000219	IMSGD
positive regulation of transcription by RNA polymerase II	GO:0045944	IDA:SGD
regulation of establishment of protein localization to chromosome	GO:0070202	IMSGD
RNA polymerase II preinitiation complex assembly	GO:0051123	IDA:SGD

Interaction

Binary Interactions
[P19659<-->P12383: PDR1]	NbExp=5	EBI-7305,EBI-13019
[P19659<-->P36956-1: SREBF1]	Xeno	NbExp=3,

Repeat regions

Repeats

>MDP31429
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     155.56|      37|      37|     416|     452|       1
---------------------------------------------------------------------------
  367-  403 (42.78/ 7.17)	NLQRAQNV....PMNIIQQQQQQNTNNNDTIATSATPNAAA
  404-  444 (57.29/11.88)	FSQQQNASsklyQMQQQQQAQAQAQAQAQAQAQAQAQAQAA
  445-  482 (55.48/11.29)	QAAQAQAQ...aQAQAQAQAQAQAQAQAQAQAQAQAQAQAH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     363.90|      92|      97|     127|     221|       2
---------------------------------------------------------------------------
  127-  181 (81.61/22.84)	......................................................................................QMF......................................LNQQAQARQQVAQQLRNQQQQ.QQQQQQQQRRQLTPQQQQ..........LVN..QMKVAP...IPKQ
  182-  265 (113.39/39.92)	L..........LQR.....IPNIPPNINtwqQVTALAQ...........QKLLTPQD........MEAAKEVYK.........ihqQLL......................................FKA............RLQQQQaQAQAQANNNNNGLPQNGN..........INN..NIN......IPQQ
  280-  360 (58.67/14.68)	L..........QQQssqntVPNVLNQIN...QIFSPEE...........QRSLLQEA........IETCKNFEKtqlgstmtepvkQSF..................................irkyINQKALRKIQALRDVKNNNN................................................
  517-  652 (59.33/14.93)	LsldasktnlrLTD.....VTNSLSN..........EEkekikmklkqgQKLFVQVSnfapqvyiITKNENFLK............EVFqlrifvkeilekcaegifvvkldtvdrliikyqkywesMRIQILRRQAI...LR..QQQ...QMANNNGNPGTTSTGN...........NN..NI...........
  655-  721 (50.90/11.31)	......................................................................................QQN......................................M.QQSLQQMQHLQQLKMQQQQ.QQQQQQQQQQQQQQQQQQqhiypsstpgVANysAMANAPgnnIP..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.06|      16|      19|    1036|    1053|       4
---------------------------------------------------------------------------
 1036- 1053 (22.06/27.63)	DDPFMTkSGaTTSEKQEV
 1056- 1071 (30.00/23.34)	EAPFLT.SG.TSSEQFNV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.18|      14|      20|     844|     857|       5
---------------------------------------------------------------------------
  844-  857 (22.04/18.01)	IENAFRKEELLLKD
  865-  878 (24.14/20.51)	ISSRFKHRQEIFKD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      76.44|      24|     267|     729|     754|       6
---------------------------------------------------------------------------
  729-  754 (36.28/28.69)	SSMDFLNSMENTPKVPVSAaaTPSLN
  756-  779 (40.15/25.41)	TINGKVNGRTKSNTIPVTS..IPSTN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      58.26|      18|      21|      76|      95|       7
---------------------------------------------------------------------------
   76-   95 (26.06/17.65)	VMRNTYNTRKNAvtAAAANN
  100-  117 (32.20/16.30)	VEQHHINNLKNS..GNSANN
---------------------------------------------------------------------------


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31429 with Med15 domain of Kingdom Fungi



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MDSMNEKVAVMRNTYNTRKNAVTAAAANNNIKPVEQHHINNLKNSGNSANNMNVNMNLNPQMFLNQQAQARQQVAQQLRNQQQQQQQQQQQQRRQLTPQQQQLVNQMK
2) MSDSKKIKVDSPDDPFMTKSGATTSEKQEVTNEAPFL
3) QQMQHLQQLKMQQQQQQQQQQQQQQQQQQQQQQQHIYPSSTPGVANYSAMANAPGNNIPYMNHKNTSSMDFLNSMENTPKVPVSAAATPSLNKTINGKVNGRTKSNTIPVTSIPSTNKKLSISNAASQQPTPRSASNTAKSTPNTNPSPLKTQTKNGTPNPNNMKTVQSPMGAQPSYNSA
4) QQQQAQAQAQANNNNNGLPQNGNINNNINIPQQQQMQPPNSSANNNPLQQQSSQNTV
5) QQQQMANNNGNPGTTSTGNNNNIATQQNMQQS
6) RKIQALRDVKNNNNANNNGSNLQRAQNVPMNIIQQQQQQNTNNNDTIATSATPNAAAFSQQQNASSKLYQMQQQQQAQAQAQAQAQAQAQAQAQAQAAQAAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAHAQHQPSQQPQQAQQQPNPLHGLTPT
67
1024
663
233
630
347
174
1060
842
289
661
507










Molecular Recognition Features






MoRF SequenceStartStop




1) IRKYI
337
341