<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31428

Description Mediator of RNA polymerase II transcription subunit 15
SequenceMNRNVDKGWQAQIRPNERQSIALQIAQTLRIISPSISEVQLMNMALSFERQAFDGASSKNEYLTTCGKKTAQLRDQIRDTLQATQMKQMPSVYNNAGNVGALPTAGPNRLANNPRVMPRLQNQNVPMQAGMQQFARNMKLTPQQRQFLLQQSQIQQQRQQQQQQSQQPQQTQQPQASSPTAPNTEANQQRSGSVPGRIVPALTQQQLNNLCNQITALLARNGNPPIPMQKLQSMPPARLISIYQNQIQKFRSLQHMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKQAPQNAFFPNPQGNVGAQSLQSMSPQDQPSTQQQQPQRTAAPPNNPNVNATNNNRINIEMLNIPVPKQLFDQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYRKHLQIILQHRQQQLNKIQNARMNSQNAPNTNKLGNPQPDNTGNPQAFSQQAFAQQQQQQQQQLHRTSNPTSASVTSQNGQQPINTKLSANAAKTNYQSYLTNKARNATQPTQPPVSQVDYSNNLPPNLDTSSTFRSSASPPSAFTKAGNEALSVPLSGARNTAASRPTNLAAGNSSASIVQQLLECGGTMGQQKMTSRIRELTERVMHSLMRPVPLDLPHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQGVFTCAPQTLAKIKEKTSRYFAFVKAQLLRLHHEVNNNNMSIQNALAHISSLRTASINQQQQPQPQSQQQQQASQFPQAPSVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAFQSLDVSQPDLQAKQKIASQVMKHGLKPEDLKLPPSKKKKIENLSTVQKPKDSVVNTPDVIMSSVDEIPSSVSPGTIAKEEGMAKAREEAIANPLKYAIDAFVAVDHEEEVSAIKSSQTPSSILKTPQSFFIPPSTPDLSFTDNKNSLSPSNILSLDGKFSFNDDSELWADLGNEINSEIGFLKEPDTMNLALDADKDKTKMQNKLTQINFDESCFLDPAIDDKDPWNEMLHEKKVLLKQLQVGNEDEDNIAFPTSTNIWQVVI
Length1063
PositionTail
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Taphrinomycotina> Schizosaccharomycetes> Schizosaccharomycetales> Schizosaccharomycetaceae> Schizosaccharomyces.
Aromaticity0.04
Grand average of hydropathy-0.766
Instability index63.90
Isoelectric point9.51
Molecular weight118451.91
Publications
PubMed=11859360
PubMed=16823372
PubMed=18257517
PubMed=20622008
PubMed=24929437

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Component of a med15-hrp1 subcomplex, linking the Mediator complex to the chromatin-remodeling activity of hrp1 at a distinct subset of hrp1-bound gene promoters. 
ECO:0000269	PubMed:20622008
GO - Cellular Component
chromatin	GO:0000785	IDA:PomBase
mediator complex	GO:0016592	ISO:PomBase
nucleus	GO:0005634	HDA:PomBase
P:positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IPomBase
GO - Biological Function
mediator complex binding	GO:0036033	IPI:PomBase
transcription coactivator activity	GO:0003713	ISO:PomBase
GO - Biological Process
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IC:PomBase
regulation of transcription by RNA polymerase II	GO:0006357	IMPomBase

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31428
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.90|      15|      15|     257|     271|       1
---------------------------------------------------------------------------
  156-  171 (27.77/ 6.06)	QQrQQQQQQSQQPQQT
  270-  285 (29.13/ 6.73)	QQqQQQQQQQQQQQQQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     629.66|     146|     190|     179|     345|       2
---------------------------------------------------------------------------
   85-  266 (202.47/59.82)	..QMKQMP.SVY..NNA....GNVGAlptagpNRLAN.NPRVMPRLQNQNvPMQAGMQQFARNMKLTPQQRqfllqqsqiqqqrqqqqqqsqqpqqtqqpqassPTAPNTEANQQ.......RSGSVPGRIVPALTQ.QQLNNLCNQI..T...ALLA......................RNGNPPIPMQKLQSMPPA..RLIS.IYQNQIQKFrsLQHMQQQQQQQQQQ
  286-  418 (201.53/53.67)	QQQQKQAPQNAFFPNPQ....GNVGA......QSLQSMSPQDQPSTQQQQ.PQRTAAPPNNPNVNATNNNR...............................inIEMLNI...............PVPKQLF......DQIPNLPPNV..KiwrDVL..............................ELGQSQRLPPEqlKLIGmLYRKHLQII..LQHRQQQLNKIQNA
  419-  544 (130.35/32.67)	RMNSQNAPNTNKLGNPQpdntGNPQA......FSQQAFAQQQQ..QQQQQ.LHRT.SNPTSASVTSQNGQQ............................pintkLSANAAKTNYQsyltnkaRNATQPTQ..PPVSQvDYSNNLPPNLdtS...STFR......................SSASPPSAFTK.......................................
  727-  836 (95.31/21.85)	.................................RTASINQQQQPQPQSQQ.QQQASQFPQAPSV..SSNVR..............................pinGSIPNA.............QPSVPGQAQPAAKA.NSVGNTSFPV..D...SKLAfqsldvsqpdlqakqkiasqvmKHGLKPEDL....KLPPS..K.............................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     122.43|      25|      33|     914|     938|       3
---------------------------------------------------------------------------
  914-  938 (43.78/20.14)	KSS...QTPSSILKTPQSF.FIPPST..PDL
  944-  971 (30.13/11.89)	KNS...LSPSNILSLDGKFsFNDDSElwADL
  973-  990 (21.84/ 6.88)	..N...EINSEI.....GF.LKEPDT..MNL
  996- 1022 (26.69/ 9.81)	KDKtkmQNKLTQINFDESC.FLDPAI..DD.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      99.48|      32|      43|     564|     600|       4
---------------------------------------------------------------------------
  564-  585 (28.46/12.16)	...................RPTNLA.......AGNSSASIVQQLLECG
  591-  631 (44.00/33.43)	QKMTSRIRELtervmhslmRPVPLD.......LPHDQKVMIASLIKSA
  632-  660 (27.02/ 7.99)	YPMFSRTNQL...............iclfyclTGNEEATI..QLIQ..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.52|      15|      17|     844|     860|       6
---------------------------------------------------------------------------
  844-  860 (19.83/15.99)	STVQKPKDSVvnTPDVI
  862-  876 (23.69/12.71)	SSVDEIPSSV..SPGTI
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31428 with Med15 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) LQAKQKIASQVMKHGLKPEDLKLPPSKKKKIENLSTVQKPKDS
2) LRDQIRDTLQATQMKQMPSVYNNAGNVGALPTAGPNRLANNPRVMPRLQNQNVPMQAGMQQFARNMKLTPQQRQFLLQQSQIQQQRQQQQQQSQQPQQTQQPQASSPTAPNTEANQQRSGSVPGRIVPAL
3) NQITALLARNGNPPIPMQKLQSMPPARLISIYQNQIQKFRSLQHMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKQAPQNAFFPNPQGNVGAQSLQSMSPQDQPSTQQQQPQRTAAPPNNPNVNATNNNRINIEMLNIPVPKQLFDQIPNLP
4) QHRQQQLNKIQNARMNSQNAPNTNKLGNPQPDNTGNPQAFSQQAFAQQQQQQQQQLHRTSNPTSASVTSQNGQQPINTKLSANAAKTNYQSYLTNKARNATQPTQPPVSQVDYSNNLPPNLDTSSTFRSSASPPSAFTKAGNEALSVPLSGARNTAASRPTNLA
5) RTASINQQQQPQPQSQQQQQASQFPQAPSVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTS
810
73
212
406
727
852
202
367
569
791










Molecular Recognition Features






MoRF SequenceStartStop




1) LISIY
239
243