<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31426

Description Mediator of RNA polymerase II transcription subunit 15
SequenceMDDWRSTISTQERSQFVTELAQILADMSRINGGEKANFNLEKLKKSAEQFESSLYASCNSKDLYLDAMRKRIAAMDSAKRKSIEAQRQKLAQAQAQAQAVQRQQQQQQQQHHMQQQGSGQQQANGIPANINAQMFLNQQAQARQQAQRQIRSTLTGQQQQQPMPQQPQPQQQQPNMMRPQLTLQQQQQLANELKVTEIPRELLQKIPNLPPGITTWPQITFWAQKNRPSQNDLIITKKVYQLHQQLLNKSKLQQSNVNRMNTSVQQPGMGVQQSPNQRMNMNQVPQQQQQVRQQQQQQQQQQQQQHKQQPQHQEPPAVLGRLNQVFTQAEQKMLYEAGKKMIENLQRSGKLPPNLDSQQQLLYIKKYMNQMVLKKMQQLGIPIPMPGSAQQQSQLLQQQQQQQQQQQQQQRQQQIPQVQQAPLQQPQVQPPQSQQAQSRRQAMGMKPTPVIPNAGVMKPQPQQTPQNIQQPMMQQQSSPPPQQQQQQQQQSKISVPRPTEQDMLALKKLNAEVTKNPVKLNDITNRLTNDQKQQIRSKLHANQQLFSSVESFIPTLYMLTRNEEHIRQLLQIRMLTREIMEKAVRGVFLVEPNVVDKVIFRYQKYYEYTKEQLLRRQQQLMTMRQMQNNNPNLTANDLLNQQQLLQNRKLNLAMQSQTQEPPQIAAQQQQPQQPISGVNNNSNMGIVLDSSLNATSRSGTNTMEFLNSPEFSAISPAVPSPNKDKKNPATRAVKGKKNSQSGIPTSNPQSNSNASVVNSRTATPTVIPGSSPMFNNKSLASGQQNSPSPKTMINSPPQQDNPYKNDELALKKMAIRTAELMSRYKHRKEVFVMSSIDLFLSTFADCLDIKDDAVDLVHKTPQPILDQINGTGKKKLSKAAQKARDQDPVEISVRNNKLLMPSKSEKTLRSFKIPIADITACFKPFADPKQLILNSTPKHDEKKRKFDDLEISPTDSSSELMSESKKVKFDSPDDMFLNDPSSVQETKPLVPSELGMYSINSKPSIPSSAGNMPAPNESTDSGMNIWDWNYWESL
Length1034
PositionTail
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kluyveromyces.
Aromaticity0.04
Grand average of hydropathy-0.970
Instability index73.04
Isoelectric point9.85
Molecular weight117853.31
Publications
PubMed=1923818
PubMed=15229592

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for transcription of genes encoding galactose-metabolizing enzymes. Essential for normal growth on nonfermentable carbon sources, for sporulation and mating. 
ECO:0000250	
ECO:0000269	PubMed:1923818
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
RNA polymerase II activating transcription factor binding	GO:0001102	IEA:EnsemblFungi
TFIIE-class transcription factor complex binding	GO:0001095	IEA:EnsemblFungi
TFIIH-class transcription factor complex binding	GO:0001097	IEA:EnsemblFungi
transcription coactivator activity	GO:0003713	IEA:EnsemblFungi
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of invasive growth in response to glucose limitation	GO:2000219	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi
regulation of establishment of protein localization to chromosome	GO:0070202	IEA:EnsemblFungi
RNA polymerase II preinitiation complex assembly	GO:0051123	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31426
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     783.27|     102|     103|     281|     382|       1
---------------------------------------------------------------------------
   90-  162 (91.36/22.12)	LAQAQA.QAQAVQR...QQQ.....QQQQQHhmQQQGS...G.Q..QQANG.I..........PANI.NAqmfLNQQ.AQARQQ....AQRQI.....R......S......TLTGQQQ....................QQ...P
  163-  258 (100.92/25.19)	MPQQPQpQQQQPNM...MRP.....QLTLQQ..QQQLA...N.E..LKVTEiP..........RELL.QKipnLPPGITTWPQITFWA.....................Q.KNRPSQNDLIITKKvYqLHQQLLNKskLQQSNVN
  281-  382 (186.83/52.82)	MNQVPQ.QQQQVRQ...QQQ.....QQQQQQ..QQQHK...Q.Q..PQHQE.P..........PAVL.GR...LNQVFTQAEQKMLYEAGKKMIENLQR......SGKLP.PNLDSQQQLLYIKK.Y.MNQMVLKK..MQQLGIP
  385-  459 (109.87/28.07)	MPGSAQ.QQSQLLQ...QQQ.....QQQQQQ..QQQQR...Q.QqiPQVQQ.A..........P..L.......QQPQVQPPQ......SQQAQSRRQA......MGMKPtPVIP..............NAGVM.K........P
  461-  554 (116.39/30.17)	PQQTPQ.NIQQPMM...QQQsspppQQQQQQ..QQQSKisvP.R..PTEQD.M..........LA.L.KK...LNAEVTKNPVK.LNDITN................RLT.N..DQKQQIRS.KL.H.ANQQLFSS..VESF.IP
  556-  631 (86.91/20.69)	LYMLTR.NEEHIRQ...LLQ.....IRMLTR..EIMEK...AvR..GVFLV.E..........PNVV.DK...V..IFRY..QKY.YEYTK......EQ.........L....LRRQQQL...........MTMRQ..MQN.NNP
  639-  744 (91.00/22.00)	LNQ..Q.QLLQNRKlnlAMQ.....SQTQEP..PQIAA...Q.Q..QQPQQ.PisgvnnnsnmGIVLdSS...LNAT.........SRSGTNTMEFLNSpefsaiSPAVPsPNKDKKNPATRAVK.G.......KK..NSQSGIP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.30|      12|      22|     940|     955|       2
---------------------------------------------------------------------------
  940-  955 (18.08/20.44)	DEKKRKFDdleiSPTD
  963-  974 (23.22/13.96)	ESKKVKFD....SPDD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.98|      19|      22|     751|     772|       3
---------------------------------------------------------------------------
  751-  772 (24.74/21.99)	NsNASVVNSRTATPTviPGS...SP
  775-  796 (29.24/14.75)	N.NKSLASGQQNSPS..PKTminSP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31426 with Med15 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AMRKRIAAMDSAKRKSIEAQRQKLAQAQAQAQAVQRQQQQQQQQHHMQQQGSGQQQANGIPANINAQMFLNQQAQARQQAQRQIRSTLTGQQQQQPMPQQPQPQQQQPNMMRPQLTLQQQQQLA
2) KKMQQLGIPIPMPGSAQQQSQLLQQQQQQQQQQQQQQRQQQIPQVQQAPLQQPQVQPPQSQQAQSRRQAMGMKPTPVIPNAGVMKPQPQQTPQNIQQPMMQQQSSPPPQQQQQQQQQSKISVPRPTEQDMLALKKLNA
3) KLNLAMQSQTQEPPQIAAQQQQPQQPISGVNNNSNMGIVLDSSLNATSRSGTNTMEFLNSPEFSAISPAVPSPNKDKKNPATRAVKGKKNSQSGIPTSNPQSNSNASVVNSRTATPTVIPGSSPMFNNKSLASGQQNSPSPKTMINSPPQQDNPYKNDELALKK
4) SKLQQSNVNRMNTSVQQPGMGVQQSPNQRMNMNQVPQQQQQVRQQQQQQQQQQQQQHKQQPQHQEPPAVLGRLN
5) TPQPILDQINGTGKKKLSKAAQKARDQDPVEISVRNN
67
374
649
250
860
190
511
812
323
896










Molecular Recognition Features






MoRF SequenceStartStop




1) LLYIKKY
2) MNIWDWNYWESL
361
1023
367
1034