<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31425

Description Isoform 3 of Mediator of RNA polymerase II transcription subunit 15
SequenceMDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQMITEALAQGGMHIRARFPPTTAVSAIPSSSIPLGRQPMAQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDERQSIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA
Length677
PositionTail
OrganismHomo sapiens (Human)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Hominidae> Homo.
Aromaticity0.03
Grand average of hydropathy-0.695
Instability index88.72
Isoelectric point9.49
Molecular weight86752.51
Publications
PubMed=11024300
PubMed=11414760
PubMed=14702039
PubMed=15461802
PubMed=15489334
PubMed=9225980
PubMed=10235267
PubMed=12167862
PubMed=15175163
PubMed=15989967
PubMed=16904669
PubMed=16630888
PubMed=18568018
PubMed=18669648
PubMed=19413330
PubMed=21269460
PubMed=23186163
PubMed=24275569
PubMed=16799563

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol- dependent gene regulation. Positively regulates the Nodal signaling pathway. 
ECO:0000269	PubMed:12167862
ECO:0000269	PubMed:16630888
ECO:0000269	PubMed:16799563
GO - Cellular Component
cytoplasm	GO:0005737	IEA:UniProtKB-SubCell
mediator complex	GO:0016592	IEA:InterPro
membrane	GO:0016020	HDA:UniProtKB
nucleoplasm	GO:0005654	IDA:HPA
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	TAS:Reactome

Interaction

Binary Interactions
[Q96RN5<-->P42858: HTT]	NbExp=3	EBI-394506,EBI-466029
[Q96RN5<-->O60244: MED14]	NbExp=2	EBI-394506,EBI-394489
[Q96RN5<-->Q71SY5: MED25]	NbExp=2	EBI-394506,EBI-394558
[Q96RN5<-->P36956-1: SREBF1]	NbExp=7	EBI-394506,EBI-948328
[Q96RN5<-->P36956-3: SREBF1]	NbExp=2	EBI-394506,EBI-948338
[Q96RN5<-->Q12772: SREBF2]	NbExp=2	EBI-394506,EBI-465059
[Q96RN5<-->Q96F44: TRIM11]	NbExp=5	EBI-394506,EBI-851809
[Q96RN5-2<-->P54253: ATXN1]	NbExp=6	EBI-11030807,EBI-930964
[Q96RN5-2<-->P42858: HTT]	NbExp=6	EBI-11030807,EBI-466029

Repeat regions

Repeats

>MDP31425
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     325.47|      43|      43|     177|     219|       2
---------------------------------------------------------------------------
  188-  233 (72.01/13.34)	QFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQH.likLHHQ...N..QQQIQQ
  234-  276 (65.34/11.32)	QQQQLQRIAQLQLQQ..QQQQQQQQQQQQQQA....LQAQ...PpiQQPPMQ
  277-  308 (66.04/11.53)	QPQPPPS...QALP......QQLQQMHHTQHH......QP...P..PQPQQP
  312-  361 (62.47/10.45)	QNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPplkfVRAPmvvQ..QPPVQP
  420-  454 (59.61/ 9.59)	QPMAQVS..QSSLPMLSSPS.P.GQQVQTPQS....M.........PPPPQP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.44|      17|      34|      82|     104|       4
---------------------------------------------------------------------------
   82-  104 (24.17/24.30)	MnalqSLTGGPAAGAAgiGMPPR
  123-  139 (35.27/17.12)	M....SLSGQPPPGTS..GMAPH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     152.65|      43|      45|     631|     675|       5
---------------------------------------------------------------------------
  600-  640 (47.60/23.79)	...........AVPTPP.PPPVPPTKQqylcQPLLDAVLANIRSpVFNHSLYR
  641-  684 (70.58/36.29)	TFV....PAMTAIHGPPiTAPVVCTRK....RRLEDDERQSIPS.VLQGEVAR
  688-  719 (34.47/11.93)	KFLvnldPSHCSNNG...TVHLIC........KLDDKDLPSVP..........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      79.13|      16|      45|     456|     476|       6
---------------------------------------------------------------------------
  456-  476 (23.36/18.59)	PQPGQPSSQPnsnVSsgPAPS
  495-  509 (27.60/ 9.16)	ARTPQNFSVP....S..PGPL
  510-  524 (28.17/ 9.52)	NTPVNPSSV....MS..PAGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.30|      16|      19|     533|     549|       7
---------------------------------------------------------------------------
  533-  549 (23.61/20.14)	LDKLKQLSKYIePLRRM
  570-  585 (28.69/19.25)	LDILTDPSKRC.PLKTL
---------------------------------------------------------------------------

Associated diseases

Disease
neurodegenerative diseases	PMID:25564654


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31425 with Med15 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) HNKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTAT
2) PLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQMITEALAQGGMHIRARFPPTTAVSAIPSSSIPLGRQPMAQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQ
3) QQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPG
70
344
257
148
538
336










Molecular Recognition Features






MoRF SequenceStartStop




NANANA