<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31422

Description Mediator of RNA polymerase II transcription subunit 15
SequenceMSSKETIPMHQRSQNVAELLTVLMDINKINGGDSTTAEKMKVHAKSFEAALFEKSSSKEEYQKTMKSKIDAMRSTRDKRKRESVGSASMMANLGQDGTNNNNNNNNNNNNNLNMAASFMGGDMFGRNQSPAQNSNANTNLNTNVGPGVNGPNGNDGTANPQMFMNQQAQARQQAAARQLKNRQMGGSSAQQQQLTQQQQQLLNQMRVAPIPKELLQRIPNLPPGVTTWEQVTALAQQNRLSAQDMSIAKDIYKIHQQYLIKAKLQQQQQRQQQQRQQGNPDVNNNMAGSNNNNNNNLPMAQQQMQQRQQQQQQSQQQQNRNPNQRHNVLSQINQMFTADEQRALLQEAMEACKNFQKTHFGGQMSDANKQAFIKKFINSKALKKLEAMRMAQGGNNNANLNKGQADMLQRQQANMQMNQQQQRAAQNQRRGPVMNDAVSQGYNNQMNSAADSTMNNSNQPMNIGNNGVNMIPNQSQQQQQTNRPKEQTPQQPQQRIQSNRSVPMLNPTPEDVEVVRRISAEAAKTQLRLTDLTNSLTPQERDEIKKRLQKNQQLFAQVSSYAPQVYLFTKSESFLKEVLQLRIFIKEILEKCSKGIYVVKLDTVDKLVIKYQKYWESMKIQLLRRQQLLQQQQQQQQQGMDPNRAQNSQQQQQQNQANMQQARNRKPTKNQTTPAIAASVAMNMNDKGASMSPALQKAGSAVPNFAQQMSPNMTPGTIPPTNVLSPHSQSHIPMVSPTMAKAASAAALKNDTASSSRRGSTKPRGKSTAPVTGKKTSNAPTPQVVPATVPSTTNLSAAGTPNIRNKSATPLTAGLSPKSTIRSNSNTALASAKTPSPMTVSIPQPGNSSVFKKEEEYLSKLQLRKEEIRFRQKQRLDILSSSPVDLFLTTVADCLGINDEEIELINKIPETTADNINNTGKKKLTKAAQKLRDKEILNVSIQVGEKDKLIMSSKAPDKVMDYSISAMSLAAVFKNLSSTGSLNNIALSGSNATTSKDIGNIYSHTGGVKRKFDEVEISPNSNGSPSASIMSESKKIKIDSPEDMFVTHSSEAAKGTNNSSLMDSGKEGSCKSMAGSATEVNDTSIWDWNFWTSIE
Length1095
PositionTail
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Nakaseomyces> Nakaseomyces/Candida clade.
Aromaticity0.04
Grand average of hydropathy-0.883
Instability index53.03
Isoelectric point9.90
Molecular weight121150.76
Publications
PubMed=15229592

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 
ECO:0000250
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
RNA polymerase II activating transcription factor binding	GO:0001102	IEA:EnsemblFungi
TFIIE-class transcription factor complex binding	GO:0001095	IEA:EnsemblFungi
TFIIH-class transcription factor complex binding	GO:0001097	IEA:EnsemblFungi
transcription coactivator activity	GO:0003713	IEA:EnsemblFungi
GO - Biological Process
cellular response to drug	GO:0035690	IMCGD
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of invasive growth in response to glucose limitation	GO:2000219	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi
regulation of establishment of protein localization to chromosome	GO:0070202	IEA:EnsemblFungi
response to drug	GO:0042493	IDA:CGD
RNA polymerase II preinitiation complex assembly	GO:0051123	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31422
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     336.50|      35|      36|      99|     133|       1
---------------------------------------------------------------------------
   99-  133 (62.76/25.71)	NNNNNNN.....NNNNNNL........................NMAASFMGGDMFGRNQ.SPAQ.N
  179-  199 (28.00/ 6.76)	LK.........................................NRQ...MGGSSAQQQQlTQQQ.Q
  284-  314 (54.92/21.44)	NNMAGSN.....NNNNNNL........................PMAQQ....QMQQRQQ.QQQQ.S
  316-  368 (29.12/ 7.37)	QQQNRNP.....NQRHNVLsqinqmftadeqrallqeameackNFQKTHFGGQM......SDA..N
  395-  427 (50.43/18.99)	NNNANLN.....KGQADML........................QRQQANM..QMNQQQQ.RAAQ.N
  443-  479 (42.91/14.89)	NNQMNSAadstmNNSNQPM........................NIGNN..GVNMIP.NQ.SQQQ.Q
  480-  509 (33.60/ 9.81)	QTNRPKE.....QTPQQPQ........................QRIQSNRSVPML..NP.TP....
  635-  660 (34.77/10.45)	QQQQGMD.....PNRAQN.............................S....Q.QQQQQ.NQANmQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     114.10|      23|      24|     691|     713|       2
---------------------------------------------------------------------------
  691-  713 (40.99/22.24)	MSPALQKAGSAVPNFAQQMSPNM
  724-  739 (28.66/13.28)	LSPHSQ...SHIP....MVSPTM
  743-  763 (19.85/ 6.87)	ASAAALKNDTASSSRRGSTKP..
  769-  788 (24.60/10.32)	.APVTGKKTSNAP..TPQVVPAT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.47|      20|      23|    1011|    1033|       3
---------------------------------------------------------------------------
 1011- 1030 (35.17/29.37)	KFDEVE......ISPNSNGSPSASIM
 1037- 1062 (27.30/11.95)	KIDSPEdmfvthSSEAAKGTNNSSLM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.78|      13|      35|     888|     902|       5
---------------------------------------------------------------------------
  888-  902 (19.16/17.50)	LTTVADCLgiNDEEI
  924-  936 (20.62/11.50)	LTKAAQKL..RDKEI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     104.80|      35|     347|     201|     276|       6
---------------------------------------------------------------------------
  143-  177 (64.84/39.00)	NVGPGV............NGPNGNDGTANP.......QMFMN...QQAQARQQAAAR
  220-  276 (39.96/39.80)	NLPPGVttweqvtalaqqNRLSAQDMSIAKdiykihqQYLIKaklQQQQQRQQQQRQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.55|      16|      38|     334|     349|       7
---------------------------------------------------------------------------
  334-  349 (28.10/15.44)	QMFTAD..EQRALLQ.EAM
  370-  388 (17.45/ 7.11)	QAFIKKfiNSKALKKlEAM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.55|      15|      28|     535|     549|       9
---------------------------------------------------------------------------
  535-  549 (26.15/17.62)	SLTPQ....ERDE..IKKRLQ
  560-  580 (15.40/ 7.13)	SYAPQvylfTKSEsfLKEVLQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      40.01|      12|      25|     806|     817|      10
---------------------------------------------------------------------------
  806-  817 (20.35/13.02)	KSATPLTAGLSP
  824-  835 (19.66/12.29)	NSNTALASAKTP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31422 with Med15 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) KLQQQQQRQQQQRQQGNPDVNNNMAGSNNNNNNNLPMAQQQMQQRQQQQQQSQQQQNRNPNQRHNVLSQINQMFT
2) NGGDSTTAEKMKVHAKSFEAALFEKSSSKEEYQKTMKSKIDAMRSTRDKRKRESVGSASMMANLGQDGTNNNNNNNNNNNNNLNMAASFMGGDMFGRNQSPAQNSNANTNLNTNVGPGVNGPNGNDGTANPQMFMNQQAQARQQAAARQLKNRQMGGSSAQQQQLTQQQQQLLNQMRVAPIPKELLQRIPNLPPGVTTWEQVTALAQQNRLSAQDMSI
3) QEAMEACKNFQKTHFGGQMSDANKQAFIKKFINSKALKKLEAMRMAQGGNNNANLNKGQADMLQRQQANMQMNQQQQRAAQNQRRGPVMNDAVSQGYNNQMNSAADSTMNNSNQPMNIGNNGVNMIPNQSQQQQQTNRPKEQTPQQPQQRIQSNRSVPMLNPTPEDVEVVRR
4) QQQQQQQQQGMDPNRAQNSQQQQQQNQANMQQARNRKPTKNQTTPAIAASVAMNMNDKGASMSPALQKAGSAVPNFAQQMSPNMTPGTIPPTNVLSPHSQSHIPMVSPTMAKAASAAALKNDTASSSRRGSTKPRGKSTAPVTGKKTSNAPTPQVVPATVPSTTNLSAAGTPNIRNKSATPLTAGLSPKSTIRSNSNTALASAKTPSPMTVSIPQPGNSS
263
30
346
630
337
247
517
849










Molecular Recognition Features






MoRF SequenceStartStop




NANANA