<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31416

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTTIGSPQMLANEERLSNEMHALKNRSEQNGQEQQGPVKNTQLHGPSATDPETTATQKESLEMVPKDTSAATMTSAPPPALPHVEINQVSLALVIRNLTVFTMKELAQYMKTNVHTQANEPNSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTIKQNNFHVLIDLLNWFRTTNMNVNNCIWALKSSLNSMTNAKLPNVDLVTALEVLSLGRPNLPTHNFKLSGVSNSMDMVDGMAKVPIGLILQRLKDLNLTVSIKIALMNIPKPLNSYHIKNGRIYFTVPNEFEIQLSTVNRQSPLFFVDLKLLFNTEAEQTVSAVTEATSTNGDSENNEENSSSNGNNLPLNKPRLEKLINEILLKSNDPLLSLYNFLHKYVLTLQLYMVHREFLKLANGGKFSKSNLIHNYDSKKSTITVRYWLNGKMDSKGKITIGIQRTTESLILKWDNQSASRAKNMPVIYNNIVSNIEGILDEIMFNHARIIRSELLARDIFQEDEENSDVLLFQLPTTCVSMAPIQLKIDLLSGQFYFRNPTPLLSNYASKINRAEGPEELARILQQLKLDKIIHVLTTMFENTGWSCSRIIKIDKPIRTQVNTGGESVVKKEDNKYAIAGNSTTNSDVSLLLQRDLFIRLPHWPLNWYLILSIISSKTSCVVEKRIGKIVSQRGKWNLKYLDNSNVMTVKLESITYQKIMILQRTILNRIINHMLIDSLNQLEIRNKICSSEMINEQKLPQYIIQGSNTNDNISIITLELESFLEGSKALNSILESSMFLRIDYSNSQIRLYAKFKRNTMMIQCQIDKLYIHFVQEEPLAFYLEESFTNLGIIVQYLTKFRQKLMQLVVLTDVVERLHKNFESENFKIIALQPNEISFKYLSNNDEDDKDCTIKISTNDDSIKNLTVQLSPSNPQHIIQPFLDNSKMDYHFIFSYLQFTSSLFKALKVILNERGGKFHESGSQYSTMVNIGLHNLNEYQIVYYNPQAGTKITICIELKTVLHNGRDKIQFHIHFADVAHITTKSPAYPMMHQVRNQVFMLDTKRLGTPESVKPANASHAIRLGNGVACDPSEIEPILMEIHNILKVDSNSSSS
Length1082
PositionTail
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces.
Aromaticity0.07
Grand average of hydropathy-0.251
Instability index39.18
Isoelectric point8.87
Molecular weight123356.73
Publications
PubMed=2196447
PubMed=9169871
PubMed=24374639
PubMed=17322287
PubMed=7479899
PubMed=11383511
PubMed=7635307
PubMed=14562095
PubMed=14562106
PubMed=14623974
PubMed=15175151
PubMed=14749387
PubMed=15477388
PubMed=16002404
PubMed=16076843
PubMed=15665377
PubMed=16263706
PubMed=16630888
PubMed=17192271
PubMed=17330950
PubMed=18407956
PubMed=12191485

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. 
GO - Cellular Component
core mediator complex	GO:0070847	IDA:SGD
mediator complex	GO:0016592	IBA:GO_Central

GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IPI:SGD
TFIIB-class transcription factor binding	GO:0001093	IDA:SGD
transcription coregulator activity	GO:0003712	IBA:GO_Central

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IMSGD
positive regulation of transcription by RNA polymerase II	GO:0045944	IMSGD
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31416
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     577.63|     156|     160|     752|     911|       1
---------------------------------------------------------------------------
  614-  758 (144.71/79.05)	....................NSDVSLL...............LQRD.LFIRL.PHWPLNWYLILSIissktscvveKRIGKIVSQ...RGKWNLKYLDnSNVMTVKLESItYQ.......KIMILQrtilnriINHMLIDSLNQLEIRNKICSSEM.INEQKLP.QYIIQGSNTNDNiSIITLELesfleGSKA
  759-  916 (250.03/138.50)	LNSILE..SSMF..LRIDYSNSQIRLYAKFKRN....TMMIQCQIDkLYIHFVQEEPLAFYLEESF..........TNLGIIVQY...LTKFRQKLMQ.LVVLTDVVERL.HKNFESENFKIIALQ.......PNEISFKYLSNNDEDDKDCTIKISTNDDSIK.NLTVQLSPSNPQ.HIIQPFL....dNSKM
  921- 1066 (182.89/96.26)	IFSYLQftSSLFkaLKVILNERG....GKFHESgsqySTMVNIG.....LHNLNEYQIVYYNPQAG..........TKITICIELktvLHNGRDKIQF.HIHFADVA....HITTKSPAYPMMHQV.......RNQV...FMLDTKRLGTPESVKPANASHAIRlGNGVACDPSE.....IEPIL.........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.93|      18|     225|     127|     147|       3
---------------------------------------------------------------------------
   96-  116 (21.87/ 6.93)	IR..NLTVFtMKelAQYMKTNVH
  128-  147 (25.06/20.94)	IRflQLIIF.LR..TQFLKLYVL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     149.40|      40|     166|     328|     367|       4
---------------------------------------------------------------------------
  328-  367 (64.84/42.78)	ENNEENSSSNGNNLPLN.......KPRLEKLINEILLKSNDPLLSLY
  490-  536 (60.01/38.99)	QEDEENSDVLLFQLPTTcvsmapiQLKIDLLSGQFYFRNPTPLLSNY
  539-  562 (24.55/11.15)	KINRAEGPEELARI.LQ.......QLKLDKII...............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.48|      16|      28|     251|     266|       5
---------------------------------------------------------------------------
  251-  266 (26.94/16.43)	LTV....SIKIALMNIPKPL
  278-  297 (21.55/11.71)	FTVpnefEIQLSTVNRQSPL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      74.43|      23|      28|      42|      65|       6
---------------------------------------------------------------------------
   42-   65 (34.98/25.55)	TQLHGPSATDPETTATQkESLEMV
   73-   95 (39.45/24.35)	TMTSAPPPALPHVEINQ.VSLALV
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31416 with Med14 domain of Kingdom Fungi
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