<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31407

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTDVAPGTMENETSKVMSLKHKAAYVPPPIPHVEMNQLPLSLLLRNLTVFTAKEISQFFKLNVHTGGKTPFEKKLELLNMILFLRNQFLKLYVLVKWAKTLKQNNFHSLIDLLNWFRNANMQVNNCTMALKQILGTMAGAKLPNPDLATSLEVLMLGRPNLPNHGFNLNGSQSDNLTIPNKLILKRLRDLNTCLSIKISLMTIPSQLAKYEIKDGRIVFTVKDEFELQLSTIDQNSPLFFVNINLLFNERLPLNLARLEKHINEILFKSTNPLCLVYQFLHKYILTLQMYMIHVELNALGLNGKYSGGHLVHNYDSKKQIITIKYWLQSKIANTCKCVVGMDKETESIVLEWQNPDVNKETVTTRYHGLLNNIESIIDEITFNHAQMIRADLLRTDTFQEDNEDTTSTSLLFQIPTTCATVSQIQLKIDQVSGIFYFHNPSNLLLSYARQINQSSNTQDLITVLGRLKLDKVDSILRHMLDKTGWICSDVVRLKSSIVPSTDSTFSKDIFVKLKDWPSNWFLVLTIISSTNTCIVEKRIGKILSVKGVWKLKYMDKKNVITSKLDTMTYPKMLTLQISILNKIVNHMIIDSLNELKIKNKICTFTTEDSNILPPYIIRKDGKENTDNITVIALGLESFLEGSKALNTILESSMLLKIDYQKMDIELYGKFKTDNEMIRCQCDELSIKFLEEDSLSFFMSENFGNLNDIMLYLSKFRKKLTQLIALTDVMDTLHSNFQSMDFRVVELRPNEIRFKYLPMKDSPDNEDCVIRIVTNQEKVEKLDIKLSEHNPQAMIQRFLDEKEGFAHNFIFHYLQFTLPLFRASKEADYTSSSPSTVKMHLFMHSLQEFHFLYRNHKVGGELSFIIQLRSVVRTRHQENTQYFVRFAQDYSQPQLQQQHPLSKAIAEIQKNAFSLALLKEQNSNTTSSTTTAGTATAGATMISTASSNKLLDLQDSSPTNAAVDWSSIKCVRLGSALACSHDQILPLLLQFHKSISDC
Length998
PositionTail
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Kluyveromyces.
Aromaticity0.08
Grand average of hydropathy-0.164
Instability index38.45
Isoelectric point8.59
Molecular weight114178.02
Publications
PubMed=15229592

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP31407
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      98.89|      32|      40|      13|      47|       1
---------------------------------------------------------------------------
   13-   47 (47.50/34.23)	ETSKVMSLK.HKAAYVPPPiPHVEMnqLPLSLLLRN
   55-   87 (51.39/27.23)	EISQFFKLNvHTGGKTPFE.KKLEL..LNMILFLRN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.06|      11|      40|     426|     436|       2
---------------------------------------------------------------------------
  426-  436 (19.19/13.25)	QLKIDQVSGIF
  467-  477 (17.87/11.80)	RLKLDKVDSIL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.20|      14|      41|     313|     326|       3
---------------------------------------------------------------------------
  313-  326 (25.18/17.57)	HNYDSKKQIITIKY
  354-  367 (25.02/17.42)	QNPDVNKETVTTRY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     100.31|      30|      41|     732|     761|       4
---------------------------------------------------------------------------
  732-  761 (51.67/33.05)	TLHSNFQSMDFRVVELRPNEIRFKYLPMKD
  774-  803 (48.64/30.69)	TNQEKVEKLDIKLSEHNPQAMIQRFLDEKE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     366.15|     123|     403|     101|     302|       5
---------------------------------------------------------------------------
  168-  302 (190.48/234.33)	NLNGSQSDNLTIPNKLILKrLRDLNtclsiKI.SLMTIPSqLAKYEIKDGRIVFTVKDEFELQLSTIDQNSPLFFVNINLlfnerLPLNLAR.LE..KHINEIL....................FKSTNPL..C....LVYQFLHKYILTLQMYMIHVELNALGL
  559-  711 (175.67/88.87)	NVITSKLDTMTYPKMLTLQ.ISILN.....KIvNHMIIDS.LNELKIKNKICTFTTEDSNILPPYIIRKDGKENTDNITV.....IALGLESfLEgsKALNTILessmllkidyqkmdielygkFKTDNEMirCqcdeLSIKFLEEDSLSFFMSENFGNLNDIML
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.27|      18|     279|     105|     122|       6
---------------------------------------------------------------------------
   88-  105 (29.72/17.42)	QFLKLYVLVKWAKTLKQN
  106-  123 (32.55/19.75)	NFHSLIDLLNWFRNANMQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.34|      18|     481|     486|     503|       7
---------------------------------------------------------------------------
  486-  503 (33.05/22.09)	WICSDVVRLKSSIVPSTD
  965-  982 (34.29/23.18)	WSSIKCVRLGSALACSHD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31407 with Med14 domain of Kingdom Fungi
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