<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31403

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPTPLPLEQMPGVGGGGGGYLPAAQEGGPRINTMSMSVLIDFIIQRTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVEDKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDDNNITVEEYVPGVKLTVSYWRDLKSELGYRLTVQSDPSEIGRPLAVVHVPSLGAKESAEVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDCEFNLQGTPAILTVPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKTLQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFVAYQKDELDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGTEVDAEVSPHKRKSNGDLLAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQTNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYDFSKTVEDLLNDWSKIVYLYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTFVGGMSAVNGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGIPRPQVPVLSFCVLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERNVVEEFTPIQGLKAFLSKYVDESAVYRGRASHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHMVHSPGPNTLYMQSHQDSPFTAMSPANNNWPGSPSMPRPSPRPGQSPDHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPDMSPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTLHLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQIMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITRIPYGAVIGVGKDWKDSPSLVLPIVYDIQTNVTQLAERTGQVISPTMTAASTLLRRFAEFNAQQNQCTLFPAITDLLTNLQLAAEMPQPPPNQSIGPPVGVGVGVGSSPNPMMPMQQLPQQVGPQGPVGPGGYPQMGPNPGGPQ
Length1553
PositionTail
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.07
Grand average of hydropathy-0.253
Instability index53.26
Isoelectric point8.17
Molecular weight172571.44
Publications
PubMed=10731132
PubMed=12537572
PubMed=12537569
PubMed=11259581
PubMed=16751183
PubMed=18327897

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA, MtnB and MtnD genes. 
ECO:0000269	PubMed:11259581
ECO:0000269	PubMed:16751183
GO - Cellular Component
core mediator complex	GO:0070847	IPI:FlyBase
mediator complex	GO:0016592	IMP:UniProtKB
GO - Biological Function
transcription coregulator activity	GO:0003712	IMP:UniProtKB
GO - Biological Process
C:mediator complex	GO:0016592	IMUniProtKB
F:transcription coregulator activity	GO:0003712	IMUniProtKB
regulation of transcription by RNA polymerase II	GO:0006357	IMUniProtKB
regulation of transcription initiation from RNA polymerase II promoter	GO:0060260	ISS:FlyBase

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP31403
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.93|      16|      23|     784|     804|       1
---------------------------------------------------------------------------
  784-  804 (17.75/27.70)	VYLtYEQAnYDFSktvEDLLN
  810-  825 (31.18/19.90)	VYL.YTLV.YDFA...EQLLN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     478.68|     191|     505|     829|    1051|       2
---------------------------------------------------------------------------
  316-  431 (74.59/19.50)	YVPGVKLTVS.YWrDLKSElGYRLTVQSDPSEI.GRPLAvvhvpslgakesaevadRAVRSEHLSMERLIVHT..VYIRSVSRLSDL.KLEFQAFLkdvdcefnlqGTPAIlTVPVLS.......PCL.....................................................................................................................
  848- 1050 (302.15/158.05)	YGPKKEVSCNiYW.SVQSH.GFRLTFVGGMSAVnGHSMM.................RDQLAQHLNQQHSITQIaqILHETYNPLSSIaKLPVLPFL..........GIPRP.QVPVLSfcvlaQSPCLI.RLTYQ.AVYCLELRFRANRLVSIRDGASSRFERNVvEEFTPIQGLKAFLSKYVDESAVYRGRASHEDD...NPLSPMGMEDNFGGPSSVAGVSAG.GSSPfLGTG......MRGP
 1059- 1104 ( 2.41/18.75)	.............................................................................................................................................................................................RFPAPHTPPsssNPHTPASP...H..PSAGAG...G.GSGP.QGHGnfnltsPPAP
 1427- 1520 (99.53/30.24)	...........................................................................................................................DSPSLVlPIVYDiQTNVTQLAERTGQVISPTMTAASTLLRRF.AEFNAQQNQCTLFPAITDLLTNLQLAAE..........MPQPPPNQSIGP..PVGVGVGvGSSP...............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     205.24|      47|      54|    1106|    1152|       4
---------------------------------------------------------------------------
 1106- 1132 (44.97/15.76)	..............................MPHPSPSGLMPSSPLN.PQPSP........HMVHSP
 1133- 1174 (71.62/29.51)	GPNTLYMQSHQDSPFTAMSP......annnWP.GSPS..MP.......RPSP........RPGQSP
 1188- 1250 (59.22/23.11)	GTDRGGSRGTLNRPWAGAVPtllthealetLCRPSP...YPNKDINvPDMSPlerflgcvYMRRQL
 1530- 1551 (29.43/ 7.74)	...................................PQQVGPQGPVG.PGGYP........QMGPNP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     144.47|      46|      70|     118|     167|       5
---------------------------------------------------------------------------
  118-  167 (66.05/61.80)	TADMLARMsRETLVRARLPNFHIPAAVEV.LTTGTYNrlPTcIRERIVPAD
  191-  237 (78.42/54.25)	TGKLLPQM.REFRIRNGRVTFEVKHEFSVsLTVMGDN..PT.VPWRLLDID
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.40|      15|     762|     491|     513|       6
---------------------------------------------------------------------------
  494-  508 (26.78/30.58)	LQHLPATATETLPFL
  647-  661 (28.62/ 7.66)	LAHVVAMCDEKIPFM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.47|      13|     505|     827|     839|       7
---------------------------------------------------------------------------
  827-  839 (23.55/16.07)	RLSLCDMLVVKSY
 1311- 1323 (21.92/14.39)	QLSQDDLLVIEQY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.80|      19|      22|    1346|    1365|      12
---------------------------------------------------------------------------
 1346- 1365 (29.31/29.26)	NLPAQVLKDFVQIMrLDLKP
 1366- 1384 (37.50/31.35)	ELGGDQLKWTVQIC.LRMPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      37.14|      11|      22|    1390|    1401|      13
---------------------------------------------------------------------------
 1390- 1401 (16.88/11.74)	VPSGnACVVMGR
 1413- 1423 (20.26/ 9.39)	IPYG.AVIGVGK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP31403 with Med14 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) QLAAEMPQPPPNQSIGPPVGVGVGVGSSPNPMMPMQQLPQQVGPQGPVGPGGYPQMGPNPGGPQ
2) YRGRASHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHMVHSPGPNTLYMQSHQDSPFTAMSPANNNWPGSPSMPRPSPRPGQSPDHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVP
1490
1002
1553
1205










Molecular Recognition Features






MoRF SequenceStartStop




NANANA