<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31383

Description Mediator of RNA polymerase II transcription subunit 13
SequenceMTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADPILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLAAAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNSYKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGAAPSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERLAFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAAAAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPDSFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTASGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTNTIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPSNSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPPQQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYTGDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLNGPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGRNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAVFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIANVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTAFHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDWLRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGIISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLFWDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDILNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA
Length2618
PositionMiddle
OrganismDrosophila melanogaster (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.06
Grand average of hydropathy-0.380
Instability index50.32
Isoelectric point6.09
Molecular weight280020.50
Publications
PubMed=11090137
PubMed=11171343
PubMed=10731132
PubMed=12537572
PubMed=12537569
PubMed=12835386
PubMed=16751183
PubMed=17290221
PubMed=18327897

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for leg and eye development and macrochaete specification or differentiation. Negatively regulates sex comb development. Required for activated transcription of the MtnB and MtnD genes. 
ECO:0000250	
ECO:0000269	PubMed:11090137
ECO:0000269	PubMed:11171343
ECO:0000269	PubMed:12835386
ECO:0000269	PubMed:16751183
ECO:0000269	PubMed:17290221
GO - Cellular Component
core mediator complex	GO:0070847	IPI:FlyBase
mediator complex	GO:0016592	IDA:UniProtKB
nucleoplasm	GO:0005654	HDA:FlyBase
nucleus	GO:0005634	IDA:FlyBase
GO - Biological Function
transcription coregulator activity	GO:0003712	IDA:UniProtKB
GO - Biological Process
cell fate commitment	GO:0045165	IGI:FlyBase
chaeta development	GO:0022416	IMFlyBase
compound eye development	GO:0048749	IMFlyBase
imaginal disc-derived leg segmentation	GO:0036011	IMFlyBase
larval somatic muscle development	GO:0007526	IMFlyBase
positive regulation of canonical Wnt signaling pathway	GO:0090263	IMFlyBase
regulation of transcription by RNA polymerase II	GO:0006357	IDA:UniProtKB
sex comb development	GO:0045498	IMFlyBase
transcription initiation from RNA polymerase II promoter	GO:0006367	ISS:FlyBase
wing disc dorsal/ventral pattern formation	GO:0048190	IGI:FlyBase

Interaction

Binary Interactions
[Q7KTX8<-->P25008: CycC]	NbExp=2	EBI-110730,EBI-195485
[Q7KTX8<-->Q9V9W8: pygo]	NbExp=2	EBI-110730,EBI-152653

Repeat regions

Repeats

>MDP31383
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.29|      16|      20|    1315|    1331|       1
---------------------------------------------------------------------------
 1315- 1331 (29.78/16.92)	PISPGPGMgPY.AARSSP
 1337- 1353 (29.51/12.27)	PTHPPPPY.PYdLAVASP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|    1329.07|     218|     219|     570|     787|       2
---------------------------------------------------------------------------
  176-  341 (150.65/52.58)	..........................................................QPCT..SSDRLFGrS..SQH......LSFSF..TFFVHG.DTVCASIDLREHP........................................A.VRPlTK.......EH....L...TEAA...AAFAAA..SSPPGSNGSAASA.GGavPNPGQdpngasmdG................................LDGGEGAAK.......AAPP..PHArKVMLAPFGIA...GILTGNSY..KASD.PIAEKILE..D...WAS...F....FPLCNKDN.T..........DVPPVVEV.VSGGHK
  356-  567 (232.39/85.25)	DMEHMEFVEMQKMQS..SVA...GAAaeSA....VLASLSCPPGAA.SAPSS.MGAAPSATAVPLGPASLN.A..PAL......AGAGV..GATAS...SAAKEISRKAAP........................................Q.AVS.AL.......ER....L...AFQP...YYDQRP..TSGFTFNTNNTHI.PA..SAAVE.........................................MPERTWQDC.......VMNTLHVDA.AAAAAAVASS...TP.....A..SGTG.SLSADGDENEQ...NKPpqdS....KQLVQQQI.QQQQQRQklWNFVDPMQKApCICTKH
  570-  787 (362.31/137.18)	NTPHGTPHGGASTYSRNSLG...GDS..SM....PVASVESPATPA.PSPHP.NSAHSQPTSVPPAEQLLN.M..SPH......APTSV..SNLQQP.PTPIDHLLDKNTP........................................A.PTP.TD.......QHDSKSI...TASP...YVHQTP..SVEPPSYTDHAAG.GG..PAGGQ........G................................LGTGPGSVP.......AQQPATPTA.ATSAGGAGSG...GPSNAIGA..NAVG.TISVKKLEMQQ...QTP...S....AAMAIKQE.PGAQGRG..VGGVTSTTEA.LNNFKR
  790- 1007 (222.17/81.16)	NPPKLTLKDPDSFYDEEWLKeviYDF..QYqeywDYSTVKRPKMEK.QR.RP.RYAK..........NLYE.G..QNHvkpvmpSPGSV..YGSQ.........LLSLDES........................................A.SQA.GG.......RGGGGQA...AGSSgggLVGIAN..STASGSDVE.ADG.SS..FFQGL........N................................IKTEPG..........LHSPSCKET.SKSSGGNSSG...GGSGSGGNlfTAEGlNPSLNDLEQLF...ETS...SndecSSVQIHTP.PDSNNPS..NGGCSAVTNT.IEDLKR
 1018- 1263 (184.76/66.21)	AAA.AAAASGAGNIQAEDL......T..KM..........FPTPPShEQQHP.NSSPCQTDVV.....MTD.L..SVD......TTTTIitSSITTTcNTTITSSINTTTT........................................AcSNP.SNsimlaaaQAPVTVVaiqTVSK...MVKQEY..NLELGSPMEE........PINDW........DyvyrppqqekfvgstryapltnlpsqtqppltLPTGCFYQP.......TWSSHKSRA.ATLAKAAAAQ...QQQHQKHQ..ALQQ.RIQLHQQKLQQlqlQNQ...Q....QQQAAAAA.AAAASGG..VGHQKHQHQH.LHDL..
 1265- 1443 (138.86/47.86)	.........................S..AA....P....RTPLTPS.TVPQPlSSGGSQ........YLLN.QlnCPQ......APPGA..S.MQQ........LMHRAGMspispgpgmgpyaarsspmsratpthppppypydlavaspA..TS.T....................SS...YLNR.PlhSQEHP.HM.HGLG.GG..ATGVV........G................................HGTGGGGHM.......GM.....VA.YTGDAGIVSG...GTAMAAG............................S....SSL..LQElPEVNSVL..VNILLYDT.A.LNVF..
 1445- 1541 (37.93/ 7.52)	......................................................................................................DHNFDSSSV........................................C.VCN.AD.......TQKIGNI...RGADsgvYV.PLP..GV...SFNPFPSGaGG..ALAGQ........R................................MLNGPSSAGfggmrmiSAFGGSP.A.SASMPGAGSGhghGPNG..GS..N................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     221.10|      67|     748|    1686|    1782|       3
---------------------------------------------------------------------------
 1686- 1766 (103.12/73.83)	FGNNGAPTASLASIANVLEF.MDAHDVIS.LALEqsrlafenqrMDNMMDfHGN.GSSSSHQQQQLTA...FHAPPPalrHKLAGIG
 2433- 2493 (95.32/43.21)	FEEEGNEDFSDADIGDL..F.WDTH...................MDRVSN.HGSpGRMDDNRSWQSAGgnnFKCTPP...QEVEEVG
 2559- 2590 (22.67/ 8.25)	SGNDH.PLDS.NLTADVLRFvLEGYNALSwLALD.....................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.28|      14|     748|    1768|    1782|       4
---------------------------------------------------------------------------
 1768- 1782 (25.66/17.15)	GRLTVHKW...PYL....PVgF
 2512- 2532 (22.62/ 9.60)	GRMPAWFWsacPHLedvcPV.F
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      70.55|      20|     865|    1542|    1561|       5
---------------------------------------------------------------------------
 1542- 1561 (38.48/24.78)	SSSCTPPSSN......PHITGYVDDD
 1562- 1587 (32.07/19.03)	PVECTCGFSAvvnrrlSHRAGLFYED
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     151.94|      44|     219|    1837|    1883|       6
---------------------------------------------------------------------------
 1837- 1883 (76.58/55.81)	GRASGQCEPQPIPSVVVGYEKDWISVAPHSIHYwdkFLLEPYSYARD
 2053- 2096 (75.36/45.88)	GTAAGETKPTLILGDPLGMGETLEDINPSAIVL...YVVNPFTFASD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.50|      19|    2027|     147|     166|       7
---------------------------------------------------------------------------
  147-  166 (30.06/25.35)	RSLL.FKALHNlMERFVLTKD
 2175- 2194 (29.45/19.29)	KSLTgFGTAAN.MEAFLKTKD
---------------------------------------------------------------------------


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31383 with Med13 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNF
2) ASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGY
3) CICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQGLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEMQQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNN
4) EADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTN
5) IQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVM
6) MEKQRRPRYAKNLYEGQNHVKPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTAS
7) NGSAASAGGAVPNPGQDPNGASMDGLDGGEGAAKAAPPP
8) RAATLAKAAAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGVGHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPPGASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAVA
9) RNKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGG
10) RVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVE
11) VPNDKTLLNPPDGSGNSHSKGGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVGKGGTAAG
501
1521
562
906
1030
831
242
1193
1600
2457
1981
553
1557
784
1000
1061
901
280
1351
1637
2490
2057










Molecular Recognition Features






MoRF SequenceStartStop




NANANA