<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP31378

Description Isoform 2 of Mediator of RNA polymerase II transcription subunit 12
SequenceMAAFGILSYEHRPLKRLRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLSDTGRRKSLMNQKDNFWLVTARSQSAINTWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAMSETKVKKKNTADPFTEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEMAIRQWDYNEKLALFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVIAAQSTSSLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSVASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPAKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGGGSSGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILKNLDQWTMRQSSLELQLMIKQTPNTEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLASLHSQVHQIVINWRENQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAQLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLQKDLGERQSDSLEKVHQLLPLPKQNRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWELFEGLKPSTAPLSWAWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQSSMGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVMGLEPSSYKTSVYRQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTSYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQQQQPHQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY
Length2169
PositionKinase
OrganismMus musculus (Mouse)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Glires> Rodentia> Myomorpha> Muroidea> Muridae> Murinae> Mus> Mus.
Aromaticity0.07
Grand average of hydropathy-0.519
Instability index57.91
Isoelectric point6.78
Molecular weight244558.35
Publications
PubMed=19468303
PubMed=15489334
PubMed=9702738
PubMed=21183079
PubMed=23806337
PubMed=24129315

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity). 
ECO:0000250
GO - Cellular Component
mediator complex	GO:0016592	IDA:MGI
nucleoplasm	GO:0005654	TAS:Reactome
nucleus	GO:0005634	ISO:MGI
ubiquitin ligase complex	GO:0000151	ISO:MGI
GO - Biological Function
beta-catenin binding	GO:0008013	IEA:InterPro
chromatin binding	GO:0003682	IDA:MGI
protein C-terminus binding	GO:0008022	ISO:MGI
protein domain specific binding	GO:0019904	ISO:MGI
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IDA:MGI
thyroid hormone receptor binding	GO:0046966	ISO:MGI
transcription coactivator activity	GO:0003713	IGI:MGI
transcription coregulator activity	GO:0003712	ISO:MGI
transcription factor binding	GO:0008134	IPI:MGI
ubiquitin protein ligase activity	GO:0061630	IEA:Ensembl
GO - Biological Process
axis elongation involved in somitogenesis	GO:0090245	IMMGI
canonical Wnt signaling pathway	GO:0060070	IMMGI
embryonic brain development	GO:1990403	IMMGI
embryonic neurocranium morphogenesis	GO:0048702	IMMGI
embryonic organ development	GO:0048568	IMMGI
endoderm development	GO:0007492	IMMGI
heart development	GO:0007507	IMMGI
neural tube closure	GO:0001843	IMMGI
neural tube development	GO:0021915	IMMGI
oligodendrocyte development	GO:0014003	IMMGI
positive regulation of transcription by RNA polymerase II	GO:0045944	ISO:MGI
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	ISO:MGI
positive regulation of transcription, DNA-templated	GO:0045893	ISO:MGI
post-anal tail morphogenesis	GO:0036342	IMMGI
protein ubiquitination	GO:0016567	ISO:MGI
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central
Schwann cell development	GO:0014044	IMMGI
somitogenesis	GO:0001756	IMMGI
spinal cord development	GO:0021510	IMMGI
stem cell population maintenance	GO:0019827	IMMGI
Wnt signaling pathway, planar cell polarity pathway	GO:0060071	IMMGI

Interaction

Binary Interactions
[A2AGH6<-->Q8R3L8: Cdk8]	NbExp=3	EBI-5744969,EBI-5745402
[A2AGH6<-->P37173: TGFBR2]	Xeno	NbExp=3,

Repeat regions

Repeats

>MDP31378
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     208.75|      16|      16|    2056|    2071|       1
---------------------------------------------------------------------------
 2056- 2071 (38.86/16.37)	QQQQQQQQQQQQQ...QQQ
 2073- 2088 (38.86/16.37)	QQQQQQQQQQQQQ...QQQ
 2094- 2109 (35.58/14.34)	QQQQQQMLRQQQQ...QQQ
 2110- 2125 (38.86/16.37)	QQQQQQQQQQQQQ...QQQ
 2126- 2143 (26.87/ 8.98)	QQQQPHQQQQQ.AappQPQ
 2144- 2159 (29.73/10.74)	PQSQPQFQRQGLQ...QTQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.98|      15|      15|     854|     868|       2
---------------------------------------------------------------------------
  854-  868 (26.85/20.12)	LEQITSFALG.MSYHL
  870-  885 (23.14/16.14)	LVQHVQFIFDlMEYSL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.23|      15|     612|      21|      35|       3
---------------------------------------------------------------------------
   21-   35 (31.21/13.79)	PPDVY..P.QDPKQKEDE
  634-  651 (22.02/ 7.67)	PPSPFddPtDDPERKEAE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.99|      16|      18|     344|     361|       4
---------------------------------------------------------------------------
  344-  361 (26.83/22.06)	STPFSDLLM.CPQhrPLVF
  364-  380 (25.16/12.83)	SCILQTILLcCPS..ALVW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.29|      13|      16|    1676|    1689|       5
---------------------------------------------------------------------------
 1676- 1689 (21.19/17.60)	EGLQVSTkQKIS.PW
 1693- 1706 (21.09/11.65)	EGLKPST.APLSwAW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     484.55|     155|     231|     169|     336|       6
---------------------------------------------------------------------------
  169-  336 (243.44/181.13)	AMSETKVKKKNTAdpFTEWTQI....ITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEM....AIRQWDYNEKLalfmfqDGMLDRHEFLTWVLECFEkIRPGEDELLKLLLPLLL..RYSGE...FVQSAYLSRRLAYFCTRRLAlQLDGVSSHSShvIAAQSTSSlPTTPAPQ
  401-  568 (241.11/145.14)	APSNLPMPEGNSA..FTQQVRAklreIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVldshSFERSDFSNSL......DSLCNRIFGLGPSKDGHE.ISSDDDAVVSLLCEWAVscKRSGRhraMVVAKLLEKRQAEIEAERCG.ESEAADEKGS..VASGSLSA.PSAPIFQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     557.89|     181|     201|    1075|    1275|       7
---------------------------------------------------------------------------
  739-  820 (70.95/31.75)	......................................................................................................................FPI.PQEESCSHEC...NQRLVvlfG.VGKQRDdaRHAI....KKITKD....ILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGED.GQKRRRNR..
 1075- 1267 (277.07/205.73)	IAILCAELTGYCKSlSAEWLGVLKALC.....CSSNNGTCG....FNDLLCNVDVSDLSfHDSLATFVAIL...IARQCLLLEDLIRcAAIPSLLNAAcsEQDSEPGArlTCRILLHLFKT.PQLNPCQSDG...NKPTV...G.IRSSCD..RHLLAASqNRIVDGavfaVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGG.GSSGRRQGGR
 1301- 1468 (209.87/116.70)	LCEDSNDLQDPVLS.SAQAQRLMQLICyphrlLDNEDGENPqrqrIKRILKNLDQWTMR.QSSLELQLMIKqtpNTEMNSLLENIAK.ATIEVFQQSA..ETGSSSGS..TASNMPSSSKTkPVLSSLERSGvwlVAPLI...AkLPTSVQ..GHVLKAA..................................GEEL..EKGQHlGSSSRKERDR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      95.64|      23|      45|    1858|    1880|       8
---------------------------------------------------------------------------
 1812- 1831 (31.25/11.65)	GPY.GVTVPPDLL.....HHANP....GSI
 1858- 1880 (42.03/18.45)	DPYRPVRLPMQKLPT...RPTYP....GVL
 1991- 2016 (22.36/ 6.05)	DPTRH....LQQRPSgyvHQQAPtyghGLT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.14|      13|      16|    1028|    1040|       9
---------------------------------------------------------------------------
 1028- 1040 (25.11/15.54)	ENPAA.HTFTYTGL
 1046- 1059 (21.03/11.76)	ENPANrYSFVCNAL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     138.02|      29|     103|    1921|    1951|      10
---------------------------------------------------------------------------
 1921- 1951 (48.73/26.12)	IQSQGMLGQSSvhQMTPSSSYGLQTSQGYTS
 1956- 1980 (44.86/18.83)	VGLQQHTGPAG..TMVP.PSYS...SQPYQS
 2026- 2051 (44.43/18.58)	LQQTPMMG.....TMTPLSAQGVQAGVRSTS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      72.58|      24|     608|    1553|    1577|      18
---------------------------------------------------------------------------
 1549- 1573 (36.76/29.31)	QRSTQQTTEWAQlLLEIIISGTV..DM
 1574- 1599 (35.83/23.35)	QSNNELFTTVLD.MLSVLINGTLaaDM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.12|      16|     101|    1731|    1747|      19
---------------------------------------------------------------------------
 1731- 1747 (28.16/22.09)	HLRPrPRAYYLEP...LPLP
 1750- 1768 (25.96/14.34)	DEEP.PAPALLEPekkAPEP
---------------------------------------------------------------------------


Explaination for Stockholm format The "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space.

#=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00)

#=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column. It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column. This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.

#=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.

Alignment of MDP31378 with Med12 domain of Kingdom Metazoa



     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AYYLEPLPLPPEDEEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQSSMGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVM
2) FSPTMPCEGKGSPSPEKPDVEKEVKPPAKEKIEGTLG
3) GAPGPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSV
4) LEPSSYKTSVYRQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQ
5) QQQPHQQQQQAAPPQPQPQSQPQFQRQGLQ
6) YVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQ
1738
688
628
1890
2127
1952
1888
724
675
1947
2156
2065










Molecular Recognition Features






MoRF SequenceStartStop




1) NIFGRY
2) QQQMLRQQ
3) QQQQYHIRQQQ
2185
2097
2085
2190
2104
2095